Identification and quantitative determination of steroidal compounds in the plant material of cabbage
Keywords:cabbage, leaves, seeds, heads, steroidal compounds, gas chromatography, mass-spectrometry
Vegetable crops are inexhaustible source of biologically active compounds. Cabbage (Brassica oleracea L.) – is a plant that has been extensively used in folk medicine of different countries of the world for the treatment of various disorders, and has anti-inflammatory, expectorant, broncholytic, diuretic, and genera tonic effect. The plant material of cabbage in not officinal in Ukraine, thus it requires a complex pharmacognostic research to be carried out.
The aim of the research. Identification and quantitative determination of steroidal compounds in the leaves, seeds and heads of cabbage.
Materials and methods. Identification of steroidal compounds and determination of their quantity in the plant material of cabbage was carried out using gas chromatography/mass-spectrometry (GC/MS) method.
The results of the study and their discussion. As a result of the carried out experiment 3 compounds of steroidal nature were identified in the leaves of cabbage of “Snow-white” variety, and 4 – of “Ukrainian autumn” and “Yaroslavna” varieties, 4 compounds in the seeds of cabbage of “Snow-white” variety, 3 – of “Ukrainian autumn” and “Yaroslavna” varieties, and 3 steroidal compounds in the heads of cabbage of all the varieties. β-Sitosterol was the dominating compound by the content in all studied samples. Its highest content was found in the leaves (2499 mg/kg) and seeds (1728 mg/kg) of cabbage of “Yaroslavna” variety, as well as in the heads (1148 mg/kg) of cabbage of “Ukrainian autumn” variety.
Conclusion. Results of the experiment can be used at the quality control methods for the cabbage plant material development as well as obtaining prospective biologically active substances from the studied plant material
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