Development of HPLC method for quantitative determination of new perspective APhI with anti-ulcer activity of trіаzоprazol

Authors

DOI:

https://doi.org/10.15587/2519-4852.2018.153387

Keywords:

1, 2, 4-triazole, triazoprazole, anti-ulcer activity, analysis, quantitative determination, HPLC method, diode-array detector

Abstract

Aim. Development of optimal, high-precision, reproducible method of quantitative determination of triazoprazole in substance using the method of high performance liquid chromatography.

Materials and methods. Physico-chemical (high-performance liquid chromatography) and mathematical (statistical processing of results) methods of research were used to achieve this goal. Chromatography was performed on a liquid chromatograph Agilent 1290 Infinity II with diode-array (LC 1290) detector and quadrupole-time-of-flight (QTOF 6530) mass analyzer. Fixed phase: chromatographic column 100×2.1 mm, filled with silica gel octadecylsilyl for chromatography Р Zorbax Eclipse Plus C18, with particle size 3,5 mkm. Mobile phase А: 0.1 % formic acid solution S in water S. Mobile phase В: 0,1 % formic acid solution S in acetonitrile S. Flow rate of the mobile phase: 0,6 ml/min. Temperature of the column: 30 ºС. Volume of the injection: 5 mkl. Detector – diode-array (DAD). Detecting: by wavelength 248 nm.

Detector settings (Q-TOF): type of ionization: positive, electrospray (+ ESI); metering mode: scanning ion with a mass from 100 to 1000 u.; voltage on the fragmentator 100 V; nitrogen temperature 350 С; nitrogen consumption 10 ml/min; nebulizer pressure 35 PSI; voltage on the capillary 4 Kv. The chromatographic separation was carried out with gradient elution on column filled with silica gel octadecylsilyl.

Results. Content of active ingredient in the substance of triazoprazole meets the requirements of regulated limits of quantitative content. Therefore, the proposed method can be used in the process of pharmaceutical development and standardization of the dosage form. Solvent and mobile phase do not interfere in conditions of the proposed method for determining the active substance. It testifies about specificity of the proposed method.

Conclusions. High-precision and reproducible method of quantitative determination of triazoprazole in substance using the method of high performance liquid chromatography was developed

Author Biographies

Narzullo Saidov, Tajik National University Rudaki str., 17, Dushanbe, Tajikistan, 734025

PhD, Associate Professor

Department of Pharmaceutical Chemistry

Natalia Smelova, National University of Pharmacy Pushkinska str., 53, Kharkіv, Ukraine, 61002

Director

Education and Research Training Center of Chromatographic Studies

Natalya Garna, National University of Pharmacy Pushkinska str., 53, Kharkіv, Ukraine, 61002

PhD, Associate Professor

Department of Pharmaceutical Chemistry

Oksana Koretnik, National University of Pharmacy Pushkinska str., 53, Kharkіv, Ukraine, 61002

PhD, Assistant

Department of Pharmaceutical Chemistry

Nataliia Khanina, National University of Pharmacy Pushkinska str., 53, Kharkіv, Ukraine, 61002

Technician

Educational and Scientific Training Center for Chromatographic Studies

Svetlana Gubar, National University of Pharmacy Pushkinska str., 53, Kharkіv, Ukraine, 61002

PhD, Head of Laboratory

State Research Laboratory for Quality Control of Medicines

References

  1. Kaur, P., Chawla, A. (2017). 1,2,4-Triazole: a review of pharmacological activities. International Research Journal of Pharmacy, 8 (7), 10–29. doi: http://doi.org/10.7897/2230-8407.087112
  2. Namratha, B., Gaonkar, S. (2014). 1,2,4-triazoles: Synthetic strategies and pharmacological. International Journal of Pharmacy and Pharmaceutical Sciences, 6 (8), 73–80.
  3. Felton, L. A. (Ed.) (2013). Remington – Essentials of Pharmaceutics. Pharmaceutical Press, 772.
  4. Georgiyants, V., Perekhoda, L., Saidov, N., Kadamov, I. (2014). Synthesis, docking studies, and biological evaluation of anti-ulcer activity of 4-allyl-5-(4-R1)-phеnylthiomethyl-1,2,4-trіаzоle-3-ylmercaptoаcetic acid derivatives. European Chemical Bulletin, 3 (5), 466–471.
  5. Georgiyants, V., Severina, G., Drogovoz, S., Timofeev, М., Saidov, N., Kadamov, І., Saaod, Khaidar (2016). Pat. No. 112867 UA. Zastosuvannia alkilovanykh pokhidnykh 1,2,4-tryazol-3-tiolu ta 1-fenil-1n-tetrazol-5-tiolu yak zasobu protyvyrazkovoi dii. MPK: С07D 249/08 (2006.01), С07D 257/04, A61K 31/41, A61K 31/4196. No. u 2013 13290; declareted: 15.11.2013; published: 10.11.2016, Bul. No. 10.
  6. Kenesov, B., Saylauhanulyi, E., Musrepov, B., Kaldarov, A. (2008). Opredelenie 1-methyl-1H-1,2,4-triazole v vodnyih obraztsah metodom vyisokoeffektivnoy zhidkostnoy hromatografii s diodno-matrichnyim detektirovaniem. Habarshyi vestnik. Seriya himicheskaya, 1 (49), 184–189.
  7. Yegemova, S., Bakaikina, N. V., Kenessov, B., Koziel, J. A., Nauryzbayev, M. (2015). Determination of 1-methyl- 1H -1,2,4-triazole in soils contaminated by rocket fuel using solid-phase microextraction, isotope dilution and gas chromatography–mass spectrometry. Talanta, 143, 226–233. doi: http://doi.org/10.1016/j.talanta.2015.05.045
  8. Tatarczak-Michalewska, M., Flieger, J., Wujec, M., Swatko-Ossor, M. (2014). Isolation and Quantitative Determination of New Tuberculostatic 1,2,4-Triazole Derivative in Urine and Plasma Samples. Journal of Analytical & Bioanalytical Techniques, 5 (4). doi: http://doi.org/10.4172/2155-9872.1000206
  9. Acanski, M., Perisic-Janjic, N., Dimova, V. (2003). Normal and reversed phase high performance liquid chromatography of some new 1, 2, 4-triazole derivatives. Acta Periodica Technologica, 34, 83–92. doi: http://doi.org/10.2298/apt0334083a
  10. Hawryl, A., Kusmierz, E., Hawryl, M., Swieboda, R., Wujec, M. (2014). Determination of Lipophilicity of New Thiosemicarbazide and 1,2,4-triazole-3-thione Derivatives Using Reversed-Phase HPLC Method and Theoretical Calculations. Journal of Liquid Chromatography & Related Technologies, 38 (4), 430–437. doi: http://doi.org/10.1080/10826076.2014.913519
  11. Varynskyi, B., Kaplaushenko, A. (2017). The development and validation of HPLC-DMD method for intermediate products impurities determination of morpholinium2-((4-(2-methoxyphenyl)-5-(pyridine-4-yl)-4H-1,2,4-triazole-3-yl)thio)acetate in bulk drug. Zaporozhye Medical Journal, 9 (3), 373–380. doi: http://doi.org/10.14739/2310-1210.2017.3.100947
  12. Blondel, A., Krings, B., Ducat, N., Pigeon, O. (2018). Validation of an analytical method for 1,2,4-triazole in soil using liquid chromatography coupled to electrospray tandem mass spectrometry and monitoring of propiconazole degradation in a batch study. Journal of Chromatography A, 1562, 123–127. doi: http://doi.org/10.1016/j.chroma.2018.05.056
  13. Sanagi, M., Heng, S., Ibrahim, W., Naim, A. (2004). High temperature high performance liquid chromatography of triazole fungicides on carbon-clad zirconia stationary phase. Malaysian Journal of Chemistry, 6 (1), 55–66.
  14. Derzhavna Pharmakopeya Ukrayini. Vol. 1 (2015). DP «Naukovo-ekspertniy farmakopeyniy tsentr». Kharkiv: DP «Ukrayinskiy naukoviy farmakopeyniy tsentr yakosti likarskih zasobiv», 1128.
  15. Saidov, N., Kadamov, I., Perehoda, L., Georgiyants, V. (2017). Razrabotka metodiki kolichestvennogo opredeleniya potentsialnogo AFI s protivoyazvennyim deystviem. Nauka i innovatsii, 4, 35–41.

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Published

2018-12-04

How to Cite

Saidov, N., Smelova, N., Garna, N., Koretnik, O., Khanina, N., & Gubar, S. (2018). Development of HPLC method for quantitative determination of new perspective APhI with anti-ulcer activity of trіаzоprazol. ScienceRise: Pharmaceutical Science, (6 (16), 41–46. https://doi.org/10.15587/2519-4852.2018.153387

Issue

No. 6 (16) (2018)

Section

Pharmaceutical Science

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