Comparative cytotoxic analysis of extracts obtained from leaves and roots of sweet flag (Acorus Calamus L.) on rat bone marrow cells in vitro

Authors

DOI:

https://doi.org/10.15587/2519-4852.2020.196405

Keywords:

cytotoxicity, red bone marrow, rats, leaf extract of Acorus Calamus, root extract of Acorus Calamus

Abstract

The aim of this study was to identify cytotoxic activity and to compare severity of cytotoxic activity of de-alcoholized alcohol-water extracts obtained from the leaves and roots of Sweet Flag (Acorus Calamus L.) on rat bone marrow cells in vitro.

Rat bone marrow cells were isolated from the femoral diaphysis of the animals under cold temperatures.

To obtain various concentrations of extract from the leaves (LEA) and roots REA, the initial extracts (concentration 1.0 g/mL) were immunologically titrated by rolling method. A quantitative assessment of cytotoxicity was made by testing plasma membrane damage in the Trypan Blue dye exclusion test.

It was found that the cytotoxicity of a substance obtained from the roots of Sweet Flag (REA) depends on the dose and time of contact with rat bone marrow. REA has a cytotoxic effect at concentrations of 0.03-1.65 g/mL. When in contact with rat bone marrow cells for 15 minutes it causes an increase in the number of dead cells by 0.48–1.56×106 per milliliter (p<0.05). For REA in all studied concentrations, an increase in the effect of cytotoxicity is shown as the time of contact of cells with the active substance increases from 15 to 90 minutes (p<0.05).

The substance obtained from the leaves of Sweet Flag (LEA) does not cause cell membrane destruction and does not have a significant effect on the viability of rat bone marrow cells in all studied concentrations

Author Biographies

Lyudmyla Derymedvid, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

MD, Professor

Department of Pharmacology

Lyudmyla Korang, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Postgraduate Student

Department of Pharmacology

Lyubov Shakina, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

PhD, Assistant

Department of Human Anatomy and Physiology

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Published

2020-02-28

How to Cite

Derymedvid, L., Korang, L., & Shakina, L. (2020). Comparative cytotoxic analysis of extracts obtained from leaves and roots of sweet flag (Acorus Calamus L.) on rat bone marrow cells in vitro. ScienceRise: Pharmaceutical Science, (1 (23), 17–22. https://doi.org/10.15587/2519-4852.2020.196405

Issue

No. 1 (23) (2020)

Section

Pharmaceutical Science

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Copyright (c) 2020 Lyudmyla Derymedvid, Lyudmyla Korang, Lyubov Shakina

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