Technological aspects of obtaining liquid extracts of black poplar buds and determining the strategy for their standardization at the pharmaceutical development stage

Authors

DOI:

https://doi.org/10.15587/2519-4852.2020.197270

Keywords:

extraction, technological process, liquid extract of black poplar buds, standardization, spectrophotometry

Abstract

The aim. Conducting research on the development of technology for obtaining liquid extract of poplar black buds, their standardization and determining the optimal parameters of the technological process of extraction, which should provide the required content of biologically active substances and pharmacological activity of the drug.

Methods. The production of liquid extracts was carried out by the method of percolation. The quantitative determination of the amount of hydroxycinnamic acid derivatives was carried out by the unified spectrophotometric method recommended by the State Pharmacopoeia of Ukraine, in terms of coffee acid, at an analytical wavelength of 509 ± 3 nm. To determine the quantitative content of the sum of flavonoids was used the method of differential spectrophotometry, flavonoids were calculated in terms of luteolin, by the reaction of flavonoids with a 2 % solution of aluminium chloride in ethanol (96 %) R at an analytical wavelength of 398 ± 3 nm.

Results. The research on the technology of obtaining liquid extracts of black poplar buds was carried out. The technological parameters of the crushed medicinal plant raw material of the black poplar buds (bulk, the coefficient of filling of the dry raw material) were determined and the extraction parameters were calculated. It is established that the process requires the use of 2nd and 3rd discharges, and returns them to the process at the first stage of the process for the series that follows. To increase the absolute content of biologically active substances, it is rational to change the extraction regimes (infusion time, extractant flow rate, temperature). The quantitative determination of extractive substances and spectrophotometric determination of the amount of hydroxycinnamic acid derivatives in terms of coffee acid, as well as the amount of flavonoids in terms of luteolin at all stages of the process of obtaining liquid extracts was done. The influence of the concentration of the extractant on the qualitative composition of the obtained extract was established. The optimal extraction conditions were selected and the technology for ethanol extracts based on ethanol (96 %) R and ethanol (70 %) R was developed from medicinal plant material of black poplar buds. To increase the absolute content of biologically active substances in liquid extracts experimentally argued to increase the infusion time to 24 hours. The liquid extracts of black poplar buds have been developed for their further screening studies.

Conclusions. As a result of the study was developed the technology of obtaining liquid extracts of poplar black buds, their standardization was carried out, the optimal parameters of the technological extraction process were determined, ethanol extracts were developed for their further screening studies

Author Biographies

Anna Bondar, Parexel International Stepana Bandery ave., 9, Kyiv, Ukraine, 04073

Clinical Research Specialist

Svitlana Gubar, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

PhD

Department of Pharmaceutical Chemistry

Nataliia Smielova, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Department of Pharmaceutical Chemistry

Tatyana Shyteyeva, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

PhD

Department of Quality Management

Olena Tkachenko, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

PhD, Associate Professor

Department of Quality Management

Liliia Kryvoruchko, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Department of Pharmaceutical Chemistry

Karina Karpenko, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Department of Pharmaceutical Chemistry

Viktoria Georgiyants, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Doctor of Pharmaceutical Sciences, Professor

Department of Pharmaceutical Chemistry

References

  1. Dmytriievskyi, D. I., Ruban, O. A., Khokhlova, L. M. (2016). Suchasnyi stan naukovykh znan spetsialnosti «Farmatsiia». Kharkiv, 98.
  2. Shostak, T. A., Kalyniuk, T. H., Gudz, N. I. (2014). Zastosuvannia roslynnykh substantsii v yakosti aktyvnykh farmatsevtychnykh inhrediientiv. Fitoterapiia. Chasopys, 3, 63–65.
  3. Harna, S. V., Vladymyrova, I. M., Burd, N. B. (2016). Suchasna fitoterapiia. Kharkiv: Drukarnia Madryd, 580.
  4. Dvulit, I. P. (2016). Aktualnist zastosuvannia fitopreparativ yak likuvalno-profilaktychnykh zasobiv u par odontolohichnykh khvorykh. Klinichna stomatolohiia, 2, 8–13.
  5. Osokir, abo topolia chorna: opys, likuvalni vlastyvosti i zastosuvannia brunok topoli chornoi. Available at: http://faqukr.ru/zdorov-ja/28327-osokir-abo-topolja-chorna-opis-likuvalni.html
  6. Ruban, O. A., Malynovska, S. A., Al-Tovaiti Murad, Mazurets, S. I. (2012). Perspektyvy stvorennia novykh oryhinalnykh preparativ na osnovi substantsii roslynnoho pokhodzhennia. Fitoterapiia. Chasopys, 2, 63–65.
  7. Braslavskii, V. B., Kurkin, V. A. (2011). Issledovanie elektronnykh spektrov flavonoidov topolia i propolisa. Medicinskii almanakh, 2 (15), 140–144.
  8. Debbache, N., Atmani, D., Atmani, D. (2014). Chemical analysis and biological activities of Populus nigra, flower buds extracts as source of propolis in Algeria. Industrial Crops and Products, 53, 85–92. doi: http://doi.org/10.1016/j.indcrop.2013.12.018
  9. Hage, S., Morlock, G. E. (2017). Bioprofiling of Salicaceae bud extracts through high-performance thin-layer chromatography hyphenated to biochemical, microbiological and chemical detections. Journal of Chromatography A, 1490, 201–211. doi: http://doi.org/10.1016/j.chroma.2017.02.019
  10. Benedec, D., Oniga, I., Muresan, B., Mot, A. C., Damian, G., Nistor, A. et. al. (2014). Contrast between Water- and Ethanol-Based Antioxidant Assays: Aspen (Populus tremula) and Black Poplar (Populus nigra) Extracts as a Case Study. Journal of Food Quality, 37 (4), 259–267. doi: http://doi.org/10.1111/jfq.12090
  11. Debbache-Benaida, N., Atmani-Kilani, D., Schini-Keirth, V. B., Djebbli, N., Atmani, D. (2013). Pharmacological potential of Populus nigra extract as antioxidant, anti-inflammatory, cardiovascular and hepatoprotective agent. Asian Pacific Journal of Tropical Biomedicine, 3 (9), 697–704. doi: http://doi.org/10.1016/s2221-1691(13)60141-0
  12. Dudonné, S., Poupard, P., Coutière, P., Woillez, M., Richard, T., Mérillon, J.-M., Vitrac, X. (2011). Phenolic Composition and Antioxidant Properties of Poplar Bud (Populus nigra) Extract: Individual Antioxidant Contribution of Phenolics and Transcriptional Effect on Skin Aging. Journal of Agricultural and Food Chemistry, 59 (9), 4527–4536. doi: http://doi.org/10.1021/jf104791t
  13. Kuś, P., Jerković, I., Jakovljević, M., Jokić, S. (2018). Extraction of bioactive phenolics from black poplar ( Populus nigra L.) buds by supercritical CO 2 and its optimization by response surface methodology. Journal of Pharmaceutical and Biomedical Analysis, 152, 128–136. doi: http://doi.org/10.1016/j.jpba.2018.01.046
  14. Mainar, A. M., Langa, E., Berrueco, B., Maestro, C., Urieta, J. S. (2008). Antioxidant Activity of Supercritical Extracts of Populus Buds. In 11th Eur. Meet. Supercrit. Fluids. New Perspect. Supercrit. Fluids Nanosci. Mater. Process, 2–7.
  15. Merghachea, D., Boucherit-Otmania, Z., Hacib, I. E., Merghachec, F. S., Chikhid, I., Boucherita, K. (2016). Antioxidant and antimicrobial activities of algerian populus nigra l. buds extracts. Bioscience & Engineering: An International Journal, 3 (1-2), 1–8.
  16. Soares, J. F., Zabot, G. L., Tres, M. V., Lunelli, F. C., Rodrigues, V. M., Friedrich, M. T. et. al. (2016). Supercritical CO 2 extraction of black poplar ( Populus nigra L.) extract: Experimental data and fitting of kinetic parameters. The Journal of Supercritical Fluids, 117, 270–278. doi: http://doi.org/10.1016/j.supflu.2016.07.005
  17. Zhang, J., Cao, X., Ping, S., Wang, K., Shi, J., Zhang, C. et. al. (2015). Comparisons of Ethanol Extracts of Chinese Propolis (Poplar Type) and Poplar Gums Based on the Antioxidant Activities and Molecular Mechanism. Evidence-Based Complementary and Alternative Medicine, 2015, 1–15. doi: http://doi.org/10.1155/2015/307594
  18. Derzhavna Farmakopeia Ukrainy. Vol. 1 (2015). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 1128.
  19. Yevsieieva, L. V., Kovpak, L. A., Vyshnevskyi, I. A. (2015). Vyznachennia tekhnolohichnykh pokaznykiv roslynnoi syrovyny brunok topoli chornoi. Upravlinnia yakistiu v farmatsii. Kharkiv: NFaU, 42.
  20. Dubashinskaia, N. V., Khishova, O. M., Shimko, O. M. (2007). Kharakteristika sposobov polucheniia ekstraktov i ikh standartizaciia (chast II). Vestnik farmacii, 2 (36), 70–79.
  21. Ruban, O. A. (Ed.) (2015). Praktykum z promyslovoi tekhnolohii likarskykh zasobiv dlia studentiv spetsialnosti «Farmatsiia». Kharkiv: NFaU, 374.

Downloads

Published

2020-02-28

How to Cite

Bondar, A., Gubar, S., Smielova, N., Shyteyeva, T., Tkachenko, O., Kryvoruchko, L., Karpenko, K., & Georgiyants, V. (2020). Technological aspects of obtaining liquid extracts of black poplar buds and determining the strategy for their standardization at the pharmaceutical development stage. ScienceRise: Pharmaceutical Science, (1 (23), 10–16. https://doi.org/10.15587/2519-4852.2020.197270

Issue

No. 1 (23) (2020)

Section

Pharmaceutical Science

License

Copyright (c) 2020 Anna Bondar, Svitlana Gubar, Nataliia Smielova, Tatyana Shyteyeva, Olena Tkachenko, Liliia Kryvoruchko, Karina Karpenko, Viktoria Georgiants

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.