Morphological changes in tissues of organs in rats with arterial hypertension (SHR) with treatment of hypotensive medicines (with ramipril and candesartan) in combination with corvitin

Abstract

To date, arterial hypertension (AH) is the most common cardiovascular disease, which is the most common cause of disability in the population due to high risk of developing complications, such as heart failure, coronary heart disease, stroke. The nature of the morphological manifestations of hypertensive disease depends on the duration and its severity, but hypertension triggers a cascade of pathological changes in the lung disease and is accompanied by disruption of the structure most sensitive to fluctuations in blood pressure organs (brain, heart, kidneys). The search for new drugs that not only reduce blood pressure but also have polytropic effects is constantly in progress. In the first place is cardioprotection, which is based on the principles of continuous monitoring of myocardial oxygenation and metabolism. Recently the attention of researchers attract bioflavonoids, namely Corvitin, which has antioxidant, prediabetics, anti-inflammatory properties. The question of influence Corwin in combination therapy with antihypertensive drugs in the treatment of hypertension has been insufficiently studied, that determines the relevance and aim of our study.

Materials and methods. The study was performed on hypertensive SHR rats, which spontaneously had hypertension. Rats were divided into groups. Experienced animal groups SHR were administered ramipril at a dose of 0.5 mg /kg, candesartan – 0.4mg /kg, and conducted combined therapy ramipril and corvitin, candesartan and corvitin. Corvitin – 50 mg /kg. Drugs were administered 1 time per day for 7 days with food (for short-term treatment) and 21 days (for long-term therapy).

To evaluate the morphological changes of the heart, kidneys and liver, we used frozen transverse sections, 10 µm thick, which were stained by the method of Ramonovsky-Gimze..

The results of the study. Corvitin when applying with ramipril and candesartan had a protective effect on the studied structures of target organs: myocardium of the ventricles, cortex of the kidney, hepatocytes. But Corvitin cardioprotective effect observed in myocardial tissue of the left ventricle during long-term combination therapy with candesartan was more significant and is characterized by the disappearance of signs of hydropic vacuolation of cardiomyocytes. Combination therapy with hypotensive drugs corvitin reduced degree of atrophy of the glomeruli, the greatest efficiency was achieved after long-term therapy. Changes in the liver tissue with the combination of drugs also had positive dynamics.

Conclusions. Combined therapy with antihypertensive drugs and corvitin significantly improves the morphological result in the studied structures not only by reducing blood pressure, but also by the pleiotropic effects of corvitin