Development of RP HPLC method for aminocaproic acid determination in a complex nasal drug

Authors

DOI:

https://doi.org/10.15587/2519-4852.2020.215397

Keywords:

RP HPLC, aminocaproic acid, dansyl chloride, complex nasal drug

Abstract

The aim of this work was to develop a method of RP HPLC for the quantitative determination of aminocaproic acid in a complex nasal drug.

Materials and methods. A solution of aminocaproic acid and a solution of a complex model mixture containing aminocaproic acid were used for the study purposes. A method used for sample preparation was derivatization of aminocaproic acid with dansyl chloride. A method of quantitative determination was RP HPLC analysis with UV detection at 288 nm.

Results. The obtained data confirm the specificity, linearity, and correctness of the method proposed for quantitative analysis. Therewith, the correlation coefficient, limit of detection, limit of quantification and relative standard deviation (RSD) are R=0.9998, LOD=4.6·10-5 g/mL, LOQ=1.4·10-4 g/mL, and RSD=1.16% respectively.

Conclusions. A method of RP HPLC for the quantitative determination of aminocaproic acid in a complex nasal drug has been developed and its validation assessment has been carried out according to the following validation parameters: specificity, accuracy, linearity, and precision (repeatability). Statistical processing of the obtained results shows that all the validation parameters studied are within the acceptance criteria

Author Biographies

Lilia Nefedova, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Postgraduate Student

Department of Management and Economics of Enterprise

Rita Sahaidak-Nikitiuk, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Doctor of Pharmaceutical Sciences, Professor

Department of of Management and Economics of Enterprise

Mykola Blazheyevskiy, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Doctor of Chemical Sciences, Professor

Department of Inorganic and Physical Chemistry

Svetlana Barnatovych, Lugansk State Medical University Budivelnykiv str., 32, Rubizhne, Ukraine, 93012

PhD, Associate Professor

Department of Drug Technology, Organization and Economics of Pharmacy

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Published

2020-10-31

How to Cite

Nefedova, L., Sahaidak-Nikitiuk, R., Blazheyevskiy, M., & Barnatovych, S. (2020). Development of RP HPLC method for aminocaproic acid determination in a complex nasal drug. ScienceRise: Pharmaceutical Science, (5 (27), 52–59. https://doi.org/10.15587/2519-4852.2020.215397

Issue

No. 5 (27) (2020)

Section

Pharmaceutical Science

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Copyright (c) 2020 Lilia Nefedova, Rita Sahaidak-Nikitiuk, Mykola Blazheyevskiy, Svetlana Barnatovych

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