Determination of carbohydrates in Agrimonia eupatoria L. herb

Authors

DOI:

https://doi.org/10.15587/2519-4852.2020.221661

Keywords:

Agrimonia eupatoria L., carbohydrates, GC/MS, water-soluble polysaccharides, pectin substances, gravimetric method

Abstract

In recent years, methods of treatment using medicinal plants and biologically active substances based on them have become increasingly common. Today, the search for plants with a long history of use and minor side effects is of interest to our society. The most promising for procurement and use in medical practice is a common throughout Ukraine – common agrimony (Agrimonia eupatoria L.). The presence of tannins, which are represented by two groups: condensed and hydrolyzed tannins is indicated. There is insufficient information in the literature on the biologically active substances of Agrimonia eupatoria L. The common agrimony herb contains 1.2–1.6 % of flavonoids, which belong to different subgroups. Thus, from the group of flavones apigenin, luteolin, luteolin-7-glycoside have been identified. The herb of Agrimonia eupatoria L. as an insufficiently studied plant raw material is also a promising object of study, including carbohydrate composition.

The aim. The aim of our study was to determine the content of carbohydrates in aerial parts of Agrimonia eupatoria L. collected from natural habitat.

Materials and methods. The determination of carbohydrates of Agrimonia eupatoria L. was carried out by gas chromatograph Agilent 6890N with a mass detector 5973 inert (Agilent Technologies, USA). The amounts of water-soluble polysaccharides and pectin substances were determined by gravimetric method.

Results. Three free carbohydrates were found in the studied grass of the Agrimonia eupatoria L., namely: D-glucose, D-galactose and D-fructose, the content of which was 15.02 mg/g, 0.28 mg/g and 12.90 mg/g, respectively. In the common agrimony, after acidic hydrolysis and derivatization with acetylated aldononitriles were identified 8 compounds. The predominant ones were D-glucose, D-galactose and D-xylose. The content of water-soluble polysaccharides and pectin substances in Agrimonia eupatoria L. was determined at 2.90 % and 8.54 % of total dry weight, respectively.

Conclusions. In completion, the present results of GC/MS indicated the presence of certain carbohydrates both in free and bound form in Agrimonia eupatoria L., that have important medicinal activity, which answered to the knowledge about the therapeutic activity of this analyzed plant. We established that in Agrimonia eupatoria L. herb the predominant monosaccharide compound was D-glucose: 23.88 mg/g and 15.02 mg/g among monosaccharides after hydrolysis and free monosaccharides respectively. Our findings propose that carbohydrates from the studied raw material can be used for creating drugs by the pharmaceutical industry, and could be used for the development of nutraceuticals

Author Biographies

Natalia Huzio, Ivano-Frankivsk National Medical University Halytska str., 2, Ivano-Frankivsk, Ukraine, 76018

Assistant

Department of Pharmacy

Andriy Hrytsyk, Ivano-Frankivsk National Medical University Halytska str., 2, Ivano-Frankivsk, Ukraine, 76018

Doctor of Pharmaceutical Sciences, Professor

Department of Pharmacy

Liudmyla Slobodianiuk, І. Horbachevsky Ternopil National Medical University of Ministry of Health of Ukraine Voli ave., 1, Ternopil, Ukraine, 46001

PhD, Assistant Professor

Department of Pharmacognosy and Medical Botany

References

  1. Slobodianiuk, L., Budniak, L., Marchyshyn, S., Sinichenko, A., Demydiak, O. (2021). Determination of Amino Acids of Cultivated Species of the Genus Primula L. Biointerface Research in Applied Chemistry, 11 (2), 8969–8977. doi: http://doi.org/10.33263/briac112.89698977
  2. Correia, H. S., Batista, M. T., Dinis, T. C. P. (2007). The activity of an extract and fraction ofAgrimonia eupatoriaL. against reactive species. BioFactors, 29 (2-3), 91–104. doi: http://doi.org/10.1002/biof.552029209
  3. Kurennov, I. P. (2007). Zolotaia entsiklopediia narodnoi meditsiny. Moscow: Martin, 546.
  4. Hrytsyk, A. R., Tsveiuk, N. P., Leibenko, N. M., Sikoryn, U. B. (2004). Perspektyvni roslyny Karpatskoho rehionu z hepatoprotektornymy ta zhovchohinnymy vlastyvostiamy. Zaporozhskyi medytsynskyi zhurnal, 2 (1), 99–100.
  5. Kuczmannová, A., Balažová, A., Račanská, E., Kameníková, M., Fialová, S., Majerník, J. et. al. (2016). Agrimonia eupatoria L. and Cynara cardunculus L. Water Infusions: Comparison of Anti-Diabetic Activities. Molecules, 21 (5), 564. doi: http://doi.org/10.3390/molecules21050564
  6. Naprasnikova, H. S., Vladymyrova, I. M., Heorhiiants, V. A. (2011). Vyznachennia yakisnoho skladu fenolnykh spoluk Agrimonia eupatoria L. Aktualni pytannia stvorennia novykh likarskykh zasobiv. Kharkiv, 97.
  7. Lee, K. Y., Hwang, L., Jeong, E. J., Kim, S. H., Kim, Y. C., Sung, S. H. (2010). Effect of Neuroprotective Flavonoids ofAgrimonia eupatoriaon Glutamate-Induced Oxidative Injury to HT22 Hippocampal Cells. Bioscience, Biotechnology, and Biochemistry, 74 (8), 1704–1706. doi: http://doi.org/10.1271/bbb.100200
  8. Shabana, M. H., Weglarz, Z., Geszprych, A., Mansour, R. M., El-Ansari, M. A. (2003). Phenolic constituents of agrimony (Agrimonia eupatoria L.) herb. Herba polonica, 49 (1-2), 24–28
  9. Kato, H., Li, W., Koike, M., Wang, Y., Koike, K. (2010). Phenolic glycosides from Agrimonia pilosa. Phytochemistry, 71 (16), 1925–1929. doi: http://doi.org/10.1016/j.phytochem.2010.08.007
  10. Kozak, I. V., Vronska, L. V., Demyd, A. E., Hroshovyy T. A. (2017). Іdentification and quantitative determination of flavonoid in Аgrimonia eupatoria. Pharmaceutical Review, 2, 38–46. doi: http://doi.org/10.11603/2312-0967.2017.2.7905
  11. Khan, A. M., Alam, A., Nazamuddin, M. D. (2013). Аgrimonia eupatoria Linn. A traditional herb and its scientific testimony – a review. American Journal of Pharmacy and Health Research, 1 (6), 25–36.
  12. Husak, L., Dakhym, I., Marchyshyn, S., Nakonechna, S (2018). Determination of sugars and fructans content in Stachys sieboldii. International Journal of Green Pharmacy, 12, 70–74. doi: http://doi.org/10.22377/ijgp.v12i01.1527
  13. Slobodianiuk, L., Budniak, L., Marchyshyn, S., Basaraba, R. (2019). Determination of amino acids and sugars content in Antennaria dioica Gaertn. International Journal of Applied Pharmaceutics, 11 (5), 39–43. doi: http://doi.org/10.22159/ijap.2019v11i5.33909
  14. Chen, Y., Xie, M. Y., Wang, Y. X., Nie, S. P., Li, C. (2009). Analysis of the monosaccharide composition of purified polysaccharides in Ganoderma atrum by capillary gas chromatography. Phytochemical analysis, 20 (6), 503–510. doi: http://doi.org/10.1002/pca.1153
  15. Stoiko, L., Dakhym, I., Pokotylo, O., Marchyshyn, S. (2017). Polysaccharides in Centaurium erythraea Rafn. International Journal of Research in Ayurveda and Pharmacy, 2, 252–255. doi: http://doi.org/10.7897/2277-4343.082122
  16. Kyslychenko, O. A., Koshovyi, O. M., Komisarenko, A. M. (2011). Aminokyslotnyi ta monotsukrovyi sklad kvitok Achilea millefolium Mill. Ukrainskyi medychnyi almanakh, 14 (2), 91–93.
  17. Mergenthaler, P., Lindauer, U., Dienel, G. A., Meisel, A. (2013). Sugar for the brain: the role of glucose in physiological and pathological brain function. Trends in Neurosciences, 36 (10), 587–597. doi: http://doi.org/10.1016/j.tins.2013.07.001
  18. Hayes, A. J., Melrose, J. (2018). Glycans and glycosaminoglycans in neurobiology: key regulators of neuronal cell function and fate. Biochemical Journal, 475 (15), 2511–2545. doi: http://doi.org/10.1042/bcj20180283
  19. Sun, F. H., Cooper, S. B., Gui, Z. (2020). Effects of carbohydrate and protein co-ingestion during short-term moderate-intensity exercise on cognitive function. The Journal of Sports Medicine and Physical Fitness, 60(4). doi: http://doi.org/10.23736/s0022-4707.20.10322-0
  20. Umbayev, B., Askarova, S., Almabayeva, A., Saliev, T., Masoud, A.-R., Bulanin, D. (2020). Galactose-Induced Skin Aging: The Role of Oxidative Stress. Oxidative Medicine and Cellular Longevity, 2020, 1–15. doi: http://doi.org/10.1155/2020/7145656
  21. Chogtu, B., Arivazhahan, A., Kunder, S. K., Tilak, A., Sori, R., Tripathy, A. (2018). Evaluation of Acute and Chronic Effects of D-Galactose on Memory and Learning in Wistar Rats. Clinical Psychopharmacology and Neuroscience, 16 (2), 153–160. doi: http://doi.org/10.9758/cpn.2018.16.2.153
  22. Pfützner, A., Demircik, F., Sachsenheimer, D., Spatz, J., Pfützner, A. H., & Ramljak, S. (2016). Impact of Xylose on Glucose-Dehydrogenase-Based Blood Glucose Meters for Patient Self-Testing. Journal of Diabetes Science and Technology, 11 (3), 577–583. doi: http://doi.org/10.1177/1932296816678428

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Published

2020-12-30

How to Cite

Huzio, N., Hrytsyk, A., & Slobodianiuk, L. (2020). Determination of carbohydrates in Agrimonia eupatoria L. herb. ScienceRise: Pharmaceutical Science, (6 (28), 35–40. https://doi.org/10.15587/2519-4852.2020.221661

Issue

No. 6 (28) (2020)

Section

Pharmaceutical Science

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Copyright (c) 2020 Natalia Huzio, Andriy Hrytsyk, Liudmyla Slobodianiuk

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