Determination of carboxylic acids content in the herbal mixtures by HPLC

Authors

DOI:

https://doi.org/10.15587/2519-4852.2021.229132

Keywords:

herbal mixtures, carboxylic acids, high performance liquid chromatography, diabetes mellitus, phytotherapy, isocitric acid, succinic acid, fumaric acid

Abstract

The aim of the study was to research the qualitative composition and to investigate the quantitative content of some carboxylic acids in the herbal mixtures with established hypoglycemic, hypolipidemic and antioxidant activity in previous studies in vivo.

Materials and methods. Studies of carboxylic acid content in the herbal mixtures were performed by HPLC analysis using Agilent Technologies 1200 liquid chromatograph (USA). Identification and quantitative analysis were performed using standard solutions of carboxylic compounds (tartaric, pyruvic, isocitric, citric, succinic and fumaric acids).

Conclusions. HPLC analysis of five samples of the herbal mixture with antidiabetic activity showed the presence of six carboxylic acids. The dominant acid in all samples was isocitric acid. Among the most important for the prevention and treatment of diabetes, high levels of succinic and fumaric acids have been identified and established. The obtained data indicate a correlation between the phytochemical composition of the studied herbal mixtures and their pharmacodynamics, which was previously established

Author Biographies

Alona Savych, I. Horbachevsky Ternopil National Medical University of Ministry of Health of Ukraine

PhD, Assistant Professor

Department of Pharnacognosy with Medical Botany

Svitlana Marchyshyn, I. Horbachevsky Ternopil National Medical University of Ministry of Health of Ukraine

Doctor of Pharmaceutical Science, Professor

Department of Pharnacognosy with Medical Botany

Roksolana Basaraba, Bukovinian State Medical University

PhD, Assistant

Department of Pharmacy

Liubomyr Kryskiw, I. Horbachevsky Ternopil National Medical University of Ministry of Health of Ukraine

PhD, Senior Lecturer

Department of Pharmaceutical Chemistry

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Published

2021-04-30

How to Cite

Savych, A., Marchyshyn, S., Basaraba, R., & Kryskiw, L. (2021). Determination of carboxylic acids content in the herbal mixtures by HPLC. ScienceRise: Pharmaceutical Science, (2 (30), 33–39. https://doi.org/10.15587/2519-4852.2021.229132

Issue

No. 2 (30) (2021)

Section

Pharmaceutical Science

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Copyright (c) 2021 Alona Savych, Svitlana Marchyshyn, Roksolana Basaraba, Liubomyr Kryskiw

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