Comprehensive study for the development of rectal suppositories with diosmin and hesperidin




suppositories, diosmin, hesperidin, hemorrhoids, biphasic-type system, pharmacological activity, release profile, technological parameters, quantitative determination


The aim. To conduct a comprehensive study of biphasic-type suppositories that contain diosmin and hesperidin.

Materials and methods. Samples of biphasic-type suppositories with a mass of 4.0 were objects of the study. Pharmacological, technological and analytical research methods were used to directly or indirectly analyze the strength and completeness of the drug activity. Thus, in this work we combined the study of the specificity of the pharmacological action of the drug, experimental verification of the quantitative content of API, analysis of the structural properties of suppositories and study of the profile of the release of active pharmaceutical ingredients.

Results. Therefore, based on the obtained data, the most effective dose was 75 mg / kg (in terms of a human dose of 300 mg per suppository). The drug in the selected dose showed a significant therapeutic effect, which significantly exceeded that of the test sample at a lower dose and the reference agent. According to the results of technological studies, it was determined that all samples of suppositories had satisfactory structural and mechanical properties. Studies of the histological structure of the mucous membrane of rats proved that there is a positive effect of treatment with suppositories with diosmin and hesperidin due to the improvement of the normal condition of the mucous membrane, the absence of edema and ulcerative defect. Research of the release profile of active pharmaceutical ingredients showed that the best percentage of release is characteristic of sample 2 (99.8 %)

Conclusions. Therefore, suppositories with diosmin and hesperidin in therapeutically dose of 75 mg/kg of animal weight can be used for further research and will be of interest in the treatment of hemorrhoids of both acute and chronic forms

Author Biographies

Yelyzaveta Borko, National University of Pharmacy

Postgraduate Student

Department of Industrial Technology of Drugs

Inna Kovalevska, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Associate Professor

Department of Industrial Technology of Drugs

Volodymyr Grudko, National University of Pharmacy

PhD, Assistant Professor

Department of Pharmaceutical Chemistry

Nadiia Kononenko, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor, Head of Department

Department Normal and pathological physiology

Mariia Velya, National University of Pharmacy

Postgraduate Student

Department of Industrial Technology of Drugs


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How to Cite

Borko, Y., Kovalevska, I., Grudko, V., Kononenko, N., & Velya, M. (2022). Comprehensive study for the development of rectal suppositories with diosmin and hesperidin. ScienceRise: Pharmaceutical Science, (1(35), 14–21.



Pharmaceutical Science