Development of a method for determining the morpholinium thiazotate using more economic and green GC/MS assay with an fid detector




HPLC, GC-FID, method development, analytical eco-scale, cost analysis, environmental, AGREE scale


One of the main steps in the pharmaceutical development of drugs is the choice of quality control methods. The correctness of the method must be confirmed by validation. In addition, manufacturers take into account various economic and environmental factors. It is especially important to determine the above aspects for domestic and promising drugs, such as the morpholinium thiazotate.

The aim. During the development of methods for the routine analysis of medicinal products, attention should be paid to efficiency of analysis, budget, as well as their impact to the environment. Because of this reason, not only new methods for routine analysis should be developed. It is important this methods must be environmentally-friendly and cost-efficient.

Materials and methods. The determination of the morpholinium thiazotate was carried out by HPLC using the SunFire C18 (150 mm × 4,6 mm, 5,0 μm) and gas chromatography with a flame ionization detector using the Rxi-5 ms (30 m long, 0.25 mm outer diameter and 0.25 μm liquid stationary phase thickness).

Results. Various chromatographic methods for the routine quantitative analysis of morpholinium thiazotate were developed. The most suitable conditions for sample preparation were established. Proposed methods were compared to find the most ecological and economic.

Conclusions. Proposed methods were accurate and reliable. However, an environmental impact assessment showed that GC-FID is a more environmentally friendly and economical method of analysis. Using 12 Principles of green analytical chemistry, the overall “analytical GREEnness (AGREE)” scale for the proposed analytical approach was computed 0.72, showing the good greener nature of the proposed analytical approach

Author Biographies

Anastasiia Belikova, National University of Pharmacy

Postgraduate Student

Department of Pharmaceutical Chemistry

Anna Materienko, Sumy State Pedagogical University named after A. S. Makarenko

PhD, Associate Professor

Department of Human Biology, Chemistry and Chemistry Teaching Methods

Lyudmila Sidorenko, National University of Pharmacy

Doctor of Pharmaceutical Sciences

Department of Pharmaceutical Chemistry

Vasyl Chornyi, Farmak JSC

Doctor of Pharmaceutical Sciences, Head of Laboratory, Postgraduate Student

Iuliia Korzh, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Postgraduate Student

Department of Social Pharmacy

Luidmula Kucherenko, Zaporizhia State Medical University

Doctor of Pharmaceutical Sciences, Professor

Department of Pharmaceutical Chemistry

Alla Kotvitska, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor

Department of Social Pharmacy

Deividas Burdulis, Lithuanian University of Health Sciences

PhD, Doctor of Pharmaceutical Sciences

Department of Pharmacognosy

Victoriya Georgiyants, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor, Head of Department

 Department of Pharmaceutical Chemistry


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How to Cite

Belikova, A., Materienko, A., Sidorenko, L., Chornyi, V., Korzh, I. ., Kucherenko, L., Kotvitska, A., Burdulis, D., & Georgiyants, V. (2022). Development of a method for determining the morpholinium thiazotate using more economic and green GC/MS assay with an fid detector. ScienceRise: Pharmaceutical Science, (3(37), 4–11.



Pharmaceutical Science