The study of carbohydrates of corn raw materials
Keywords:corn, corn silk, leaves, roots, carbohydrates, fractionation, gas chromatography/mass spectrometry
The aim. The aim of the study was to study the content of polysaccharide fractions depending on the dissolution, qualitative composition and quantitative content of sugars in corn silk, leaves and roots.
Materials and methods. To study different fractions of polysaccharides - water-soluble polysaccharides (WSPS), pectin substances (PS), hemicelluloses A (HC A) and B (HM B) used a gravimetric method based on the extraction of polysaccharide fractions with a suitable solvent followed by sedimentation and weighing of the sediment. Determination of the qualitative composition and quantitative content of monosaccharides in corn medicinal plant materials (MPM) was carried out by the method of gas chromatography-mass spectrometry (GC/MS).
Results. Conducted studies of corn silk, leaves and roots carbohydrates using the fractionation method indicate the following trend of BAS accumulation in the studied raw materials: the content of WSPS and PS in corn silk exceeded the content of these compounds in other types of raw materials – 4.07±0.14 % and 7.20±0.29 %, respectively; leaves accumulated the most HC A and HC B compared to the content of these fractions in other samples - 6.81±0.21 % and 21.20±0.84 %, respectively. It was established by the GC/MS method that 4 substances were identified in corn silk in the free state: arabinose, glucose, galactose and fructose. After hydrolysis 5 substances were identified in corm silk - arabinose, xylose, mannose, glucose, galactose. Among the free sugars 3 compounds were found in corn leaves - glucose, fructose and sucrose. After hydrolysis, 4 compounds were identified in in corn leaves – arabinose, xylose, glucose and galactose. In corn roots 3 compounds are found in a free form - glucose, fructose and sucrose. After hydrolysis 4 compounds were identified in corn roots - arabinose, xylose, glucose and galactose.
Conclusions. The obtained results indicate a significant content of polysaccharides and sugars in the raw materials of corn, which makes it possible to predict the anti-inflammatory, detoxifying, adsorbing, energetic activity of the studied types of corn plant raw materials
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