Investigation of the polyphenol composition of red oak (Quercus rubra L.) raw materials

Authors

DOI:

https://doi.org/10.15587/2519-4852.2023.277969

Keywords:

Northern Red Oak, Quercus rubra, leaves, shoots, flavonoids, catechins, hydroxycinnamic acids, high-performance liquid chromatography (HPLC)

Abstract

The aim of the work was to determine the main groups of polyphenolic compounds in Quercus rubra L. raw materials.

Materials and methods. The leaves and annual shoots of Northern Red Oak used for research were collected in August in Lisnyky village of Obuhiv district of Kyiv Oblast (Ukraine). Determination of the component composition and quantitative content of flavonoids (including separately catechins), hydroxycinnamic acids, and phenolic acids were carried out in the samples of air-shade-dried crushed raw material to a particle size of 3 mm by the method of high-performance liquid chromatography (HPLC). Agilent Technologies 1200 liquid chromatograph was used for liquid chromatography.Results. Using the HPLC method, 18 polyphenolic compounds were identified in leaves and annual shoots of Northern Red Oak, in particular, flavonoids: rutin, quercetin-3-β-glucoside, luteolin, neohesperidin; catechins: catechin, epicatechin, epicatechin gallate, gallocatechin; hydroxycinnamic acids: chlorogenic, caffeic, trans-ferulic, trans-cinnamic, p-coumaric, hydroxyphenylacetic, benzoic, syringic, sinapic acids; phenolic acid is gallic acid. The dominant component among flavonoids is rutin (323.43 mg/100 g) (in the composition of catechins, epicatechin gallate (25.45 mg/100 g) prevails); among hydroxycinnamic acids in Northern Red Oak raw materials, chlorogenic acid (139.62 mg/100 g) and sinapic acid (74.64 mg/100 g) prevail.Conclusions. The obtained results point to the prospects of further phytochemical and pharmacological studies of Quercus rubra raw materials, with the aim of creating new plant substances based on it with antioxidant, anti-inflammatory, and antiviral activity

Author Biographies

Olena Konovalova, Private Higher Educational Establishment “Kyiv Medical University”

Doctor of Pharmaceutical Science, Professor

Department of Pharmaceutical and Biological Chemistry, Pharmacognosy

Tetiana Omelkovets, Private Higher Educational Establishment “Kyiv Medical University”

Assistant

Department of Pharmaceutical and Biological Chemistry, Pharmacognosy

Iryna Hurtovenko, Private Higher Educational Establishment “Kyiv Medical University”

PhD, Associate Professor

Department of Pharmaceutical and Biological Chemistry, Pharmacognosy

Natalia Sydora, Turku University

Doctor of Pharmaceutical Science, Associate Professor, Collegium Researcher

Department of Chemistry

Mariia Kalista, National Museum of Natural History of National Academy of Sciences of Ukraine; Private Higher Educational Establishment “Kyiv Medical University”

PhD, Researcher

Department of Botany

Associate Professor

Department of Pharmaceutical and Biological Chemistry, Pharmacognosy

Olha Shcherbakova, National Museum of Natural History of National Academy of Sciences of Ukraine; Private Higher Educational Establishment “Kyiv Medical University”

PhD, Senior Researcher

Department of Botany

Associate Professor

Department of Pharmaceutical and Biological Chemistry, Pharmacognosy

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Investigation of the polyphenol composition of red oak (Quercus rubra L.) raw materials

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Published

2023-04-30

How to Cite

Konovalova, O., Omelkovets, T., Hurtovenko, I., Sydora, N., Kalista, M., & Shcherbakova, O. (2023). Investigation of the polyphenol composition of red oak (Quercus rubra L.) raw materials. ScienceRise: Pharmaceutical Science, (2(42), 75–81. https://doi.org/10.15587/2519-4852.2023.277969

Issue

No. 2(42) (2023)

Section

Pharmaceutical Science

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Copyright (c) 2023 Olena Konovalova, Tetiana Omelkovets, Iryna Hurtovenko, Natalia Sydora, Mariia Kalista, Olha Shcherbakova

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