Study of phenolic compounds of umbellate wintergreen herb and their influence on biochemical indicators of blood and urine in the rat model of chronic kidney disease

Authors

DOI:

https://doi.org/10.15587/2519-4852.2024.310744

Keywords:

Chimaphila umbellata (L.), phenolic compounds, antioxidant effect, chronic kidney disease, neuroprotective effect

Abstract

Diseases of the kidneys and urinary tract are a common problem in people of all ages. Kidneys filter blood, removing water-soluble waste from the body, maintain water-salt balance, stabilize blood pressure and PH level. Intoxication, hypothermia, injuries and other causes lead to problems with the kidneys - inflammatory disease, urolithiasis, etc. Therefore, the development of effective herbal remedies that affect the etiopathogenic factors of diseases is urgent.

The aim. Study of the profile of phenolic compounds of umbellate wintergreen, study of antioxidant and nephroprotective properties of umbellate wintergreen extract on the model of chromate-induced renal failure (chronic kidney disease) in rats.

Materials and methods. Phenolic compounds were analyzed by high-performance liquid chromatography (HPLC) using a Waters e2695 Alliance HPLC system in combination with a 2998 PDA detector (Waters, Milford, MA, USA).

Study of in vitro antioxidant activity by HPLC method for 50 % extract of umbellate wintergreen herb. A Waters 2695 chromatograph (Waters, USA) equipped with a Waters 996 diode-matrix detector was used.

To determine the nephroprotective effect, 60 outbred sexually mature rats (males), divided into six groups, were studied. Based on the results of biochemical studies, creatinine clearance and urea clearance were calculated in experimental animals.

Results. 8 phenolic compounds - apigenin, hyperoside, quercitrin, rutin, quercetin, gallic acid, guaiaverine and isoquercetin - were identified and quantified in umbellate wintergreen by HPLC. Based on the results of a preliminary assessment of the antioxidant contribution of individual phenolic compounds to the overall effectiveness of the umbellate wintergreen grass extract, a significant effect of caffeic acid derivatives and quercetin was determined.

The use of Chimaphila umbellata extract in experimental animals was marked by the normalization of diuresis, a decrease in the total protein content of urine by 1.8 times. The effect of the use of umbellate wintergreen extract was expected to be dose dependent.

Conclusions. The profile of umbellate wintergreen phenolic compounds was investigated by HPLC, and their antioxidant effect was determined. In the conditions of the development of renal failure in rats, the studied extract of Chimaphila umbellata improved the physical condition of the animals, reduced their mortality, improved the excretory function of the kidneys, normalized nitrogen and protein metabolism, contributed to the protection of the structure of the kidney tissue, and therefore had a positive effect on the course of nephropathy

Author Biographies

Oleksiy Kovregin, National University of Pharmacy

PhD Student

Department of Clinical Pharmacology

Institute for Advanced Training of Pharmacy Specialists

Dmytro Lytkin, National University of Pharmacy

PhD

Educational and Scientific Institute of Applied Pharmacy

Olha Mykhailenko, National University of Pharmacy; UCL School of Pharmacy

PhD, Associate Professor

Department of Clinical Pharmacology

Visiting Researcher

Pharmacognosy and Phytotherapy Group

Liudas Ivanauskas, Lithuanian University of Health Sciences

Doctor of Biomedical Sciences, Professor, Head of Department

Department of Analytical and Toxicological Chemistry

Tetiana Yudkevych, National University of Pharmacy

Deputy Director for Research

Educational and Scientific Institute of Applied Pharmacy

Inna Vladymyrova, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor

Department of Clinical Pharmacology

Institute for Advanced Training of Pharmacy Specialists

Larisa Khodak, Kharkiv National Medical University

Doctor of Medical Sciences, Professor

Department of Infectious and Pediatric Infectious Diseases, Parasitology, Phthisiology and Pulmonology

Volodymyr Starikov, Kharkiv National Medical University

Doctor of Medical Science, Professor, Head of Department

Department of Oncology

References

  1. Grams, M. E., Levey, S. A., Coresh, J.; Yu, A. S. L., Chertow, G. M., Luyckx, V. A., Marsden, P. A., Skorecki, K., Taal, M. W., Wasser, W. G. (Eds.) (2020). Epidemiology of Kidney Disease. Brenner and Rector’s The Kidney. Vol. 1. Philadelphia: Elsevier, 616-639.e5.
  2. Wang, H., Naghavi, M., Allen, C., Barber, R. M., Bhutta, Z. A., Carter, A. et al. (2016). Global, regional, and national life expectancy, all-cause mortality, and cause-specific mortality for 249 causes of death, 1980–2015: A systematic analysis for the Global Burden of Disease Study 2015. Lancet, 388, 1459–1544. https://doi.org/10.1016/s0140-6736(16)31012-1
  3. Luyckx, V. A., Tonelli, M., Stanifer, J. W. (2018). The global burden of kidney disease and the sustainable development goals. Bulletin of the World Health Organization, 96 (6), 414-422D. https://doi.org/10.2471/blt.17.206441
  4. Shebeko, S. K., Chernykh, V. V., Zupanets, K. O. (2020). Nephroprotective Effect of the Herbal Composition BNO 2103 in Rats with Renal Failure. Scientia Pharmaceutica, 88 (4), 47. https://doi.org/10.3390/scipharm88040047
  5. Ali, U., Khan, M. M., Khan, N., Haya, R. tul, Asghar, M. U., Abbasi, B. H. (2023). Chimaphila umbellata; a biotechnological perspective on the coming-of-age prince’s pine. Phytochemistry Reviews, 23 (1), 229–244. https://doi.org/10.1007/s11101-023-09880-1
  6. Ivanauskas, L., Uminska, K., Gudžinskas, Z., Heinrich, M., Georgiyants, V., Kozurak, A., Mykhailenko, O. (2023). Phenological Variations in the Content of Polyphenols and Triterpenoids in Epilobium angustifolium Herb Originating from Ukraine. Plants, 13 (1), 120. https://doi.org/10.3390/plants13010120
  7. Marksa, M., Radušienė, J., Jakštas, V., Ivanauskas, L., Marksienė, R. (2015). Development of an HPLC post-column antioxidant assay forSolidago canadensisradical scavengers. Natural Product Research, 30 (5), 536–543. https://doi.org/10.1080/14786419.2015.1027703
  8. Mykhailenko, O., Korinek, M., Ivanauskas, L., Bezruk, I., Myhal, A., Petrikaitė, V. et al. (2020). Qualitative and Quantitative Analysis of Ukrainian Iris Species: A Fresh Look on Their Antioxidant Content and Biological Activities. Molecules, 25 (19), 4588. https://doi.org/10.3390/molecules25194588
  9. Kozhemiakin, Yu. M., Khromov, O. S., Filonenko, M. A., Saifetdynova, H. A. (2002). Naukovo-praktychni rekomendatsii z utrymannia laboratornykh tvaryn ta roboty z nymy. Kyiv: Avitsena, 156.
  10. Stefanov, O. V. (Ed.) (2001). Doklinichni doslidzhennia likarskykh zasobiv (metodychni rekomendatsii). Kyiv: Avitsena, 528.
  11. Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. OJEU2010 (2010). L276, 33–79.
  12. Instruktsiia dlia medychnoho zastosuvannia preparatu LESPEFRYL (LESPEFRIL). Normatyvno-derektyvni dokumenty MOZ Ukrainy (2021). Available at: https://mozdocs.kiev.ua/likiview.php?id=52052
  13. Kovregin, O., Prokopiuk, V., Lytkin, D., Vladymyrova, I. (2024). Study of the influence of the extract of pipsissewa on cell cultures. ScienceRise: Pharmaceutical Science, 3 (49), 78–85. https://doi.org/10.15587/2519-4852.2024.307291
  14. Nair, A., Jacob, S. (2016). A simple practice guide for dose conversion between animals and human. Journal of Basic and Clinical Pharmacy, 7 (2), 27–31. https://doi.org/10.4103/0976-0105.177703
  15. Shtryhol, S. Iu., Lisovyi, V. M., Zupanets, I. A. et al. (2009). Metody eksperymentalnoho modeliuvannia urazhennia nyrok dlia farmakolohichnykh doslidzhen. Kharkiv: Vyd-vo NFaU, 48.
  16. Jadhav, R., Jadhav, N., Patil, C., Chaudhari, K., Wagh, J., Surana, S. (2010). Diuretic and natriuretic activity of two mistletoe species in rats. Pharmacognosy Research, 2 (1), 50–57. https://doi.org/10.4103/0974-8490.60576
  17. Naumenko, A. N., Gorelaya, M. V., Babiy, S. O. (2017). Biochemical composition of urine in rats with developed Guerin’s carcinoma and administration of cisplatin. Regulatory Mechanisms in Biosystems, 8 (1), 11–14. https://doi.org/10.15421/021702
  18. Vilkhova, I. V., Mateshuk-Vatseba, L. R., Kantser, O. V., Podoliuk, M. V., Bekesevych, A. M., Hresko, N. I. (2021). Changes in biochemical parameters of nitrogen renal function of rats with long-term administration of therapeutic doses of nalbuphine. Bulletin of Medical and Biological Research, 3 (1), 54–61. https://doi.org/10.11603/bmbr.2706-6290.2021.1.12088
  19. Indrayan, A., Malhotra, K. R. (2018). Medical biostatistics. Boca Raton: CRC Press, 685.
  20. Yu, Y., Elshafei, A., Zheng, X., Cheng, S., Wang, Y., Piao, M. et al. (2021). Chemical constituents of Chimaphila japonica Miq. Biochemical Systematics and Ecology, 95, 104219. https://doi.org/10.1016/j.bse.2020.104219
  21. Stan, D., Enciu, A.-M., Mateescu, A. L., Ion, A. C., Brezeanu, A. C., Stan, D., Tanase, C. (2021). Natural Compounds With Antimicrobial and Antiviral Effect and Nanocarriers Used for Their Transportation. Frontiers in Pharmacology, 12. https://doi.org/10.3389/fphar.2021.723233
  22. Newman, D. J., Cragg, G. M. (2020). Natural Products as Sources of New Drugs over the Nearly Four Decades from 01/1981 to 09/2019. Journal of Natural Products, 83 (3), 770–803. https://doi.org/10.1021/acs.jnatprod.9b01285
Study of phenolic compounds of umbellate wintergreen herb and their influence on biochemical indicators of blood and urine in the rat model of chronic kidney disease

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Published

2024-08-30

How to Cite

Kovregin, O., Lytkin, D., Mykhailenko, O., Ivanauskas, L., Yudkevych, T., Vladymyrova, I., Khodak, L., & Starikov, V. (2024). Study of phenolic compounds of umbellate wintergreen herb and their influence on biochemical indicators of blood and urine in the rat model of chronic kidney disease. ScienceRise: Pharmaceutical Science, (4 (50), 44–52. https://doi.org/10.15587/2519-4852.2024.310744

Issue

No. 4 (50) (2024)

Section

Pharmaceutical Science

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Copyright (c) 2024 Oleksiy Kovregin, Dmytro Lytkin, Olha Mykhailenko, Liudas Ivanauskas, Tetiana Yudkevych, Inna Vladymyrova, Larisa Khodak, Volodymyr Starikov

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