The study of hepatoprotective influence of anti-diabetic herbal tea on the histological state of liver in rats with streptozotocin nicotinamide induced diabetes type 2
DOI:
https://doi.org/10.15587/2519-4852.2016.87255Keywords:
type 2 diabetes, Streptozotocin, Nicotinamide, decoction, anti-diabetic herbal tea, histological study, hepatocytesAbstract
Aim of research was to study hepatoprotective influence of the anti-diabetic herbal tea comparing with Metformin tablets and “Arphasetin” tea during experimental type 2 diabetes using liver tissue histological research.
Materials and methods. Experimental type 2 diabetes was reproduced by the single intravenous administration of Streptozotocin (STZ “Sigma”, USA) in the dose of 65 mg/kg.
Results. It was experimentally determined that the administration of the anti-diabetic herbal tea decoction in the dose of 9 mg/kg in rats having diabetes type 2, induced by Streptozotocin in the dose of 65 mg/kg and Nicotinamide in the dose of 230 mg/kg administration, prevents microcirculation disorders and contributes preservation trabecular structure of hepatic fates. Hepatolysis and degeneration of hepatocytes signs are less expressed, glycogen is better stored in cells, and the number of lipid droplets decreases. Anisonucleosis occurs more rarely, cells nuclei are better contoured, the number of dual cells and euchromatin content increase.
Conclusion. The effect of the anti-diabetic herbal tea is similar to the effect of the reference drug Metformin and better than the effect of “Arphasetin” tea, in terms of expressiveness positive influence on diabetegen toxic-degenerative action’s morphological signs. Thus, the administration of the anti-diabetic herbal tea in the dose of 9 mg/kg decreases destructive and degenerative changes of the structural and functional organization of liver of the rats with experimental diabetes type 2References
- Wild, S., Roglic, G., Green, A., Sicree, R., King, H. (2004). Global Prevalence of Diabetes: Estimates for the year 2000 and projections for 2030. Diabetes Care, 27 (5), 1047–1053. doi: 10.2337/diacare.27.5.1047
- Turner, R. C., Cull, C. A., Stratton, I. M., Manley, S. E., Kohner, E. M., Matthews, D. R., Levy, J. C. et. al. (1995). U. K. Prospective Diabetes Study 16: overview of 6 years (therapy of type II diabetes: a progressive disease). Diabetes, 44 (11), 1249–1258. doi: 10.2337/diabetes.44.11.1249
- Babak, O. Y., Kolesnikov, N. A., Kravchenko, E. V. (2009). Liver fibrosis: Modern mechanism of presentation, methods of treatment and diagnosis. Modern gastroenterology, 2 (46), 5–17.
- Babak, O. Y. (2008). Liver disease in patients with diabetes mellitus: a modern tactics and strategy of therapy. Health of Ukraine, 6 (1), 14–15.
- Mykhalchuk, L. M., Efimov, A. S. (2010). Nonalcoholic fatty liver disease. Internat. Endocrinology Journal, 2 (26), 71–82.
- Brunt, E. (2000). Historical grading and standing for nonalcoholic steatohepatitis. Falk. Symposium. Steatohepatitis: Abstract, 14−15.
- Roberts, E. A. (2007). Pediatric nonalcoholic fatty liver disease (NAFLD): A “growing” problem? Journal of Hepatology, 46 (6), 1133–1142. doi: 10.1016/j.jhep.2007.03.003
- Zilov, A. V. (2005). The liver of metabolic syndrome and insulin resistance: a look of endocrinologist. Clinical prospects of gastroenterology, hepatology, 5, 14–18.
- Korneev, O. N., Drapkina, O. M., Bueverov, S. A., Ivashkin, V. T. (2005). Nonalcoholic fatty liver disease as a manifestation of the metabolic syndrome. Clinical prospects of gastroenterology, hepatology, 4, 21–24.
- Bueverov, S. A., Maevskaya, M. V., Ivashkin, V. T. (2001). Alcoholic liver disease. Russian Medical Journal, 3 (2), 61–65.
- Gorbenko, N. I., Poltorak, V. V., Gladkih, A. I., Ivanova, O. V. (2001). Effect of fensuxinal on Development of insulin resistance in rat with streptozotocin-induced diabetes. Experimental and Clinical Medicine, 3, 8–10.
- Gorbenko, N. I., Gorshunskaya, M. Y. (2005). Metformin is selection means to prevent and reduce the severity of heart pathology in patients with diabetes type 2. Ukrainian Medical Journal, 4 (48), 29–35.
- Masiello, P., Broca, C., Gross, R., Roye, M., Manteghetti, M., Hillaire-Buys, D. et. al. (1998). Experimental NIDDM: development of a new model in adult rats administered streptozotocin and nicotinamide. Diabetes, 47 (2), 224–229. doi: 10.2337/diabetes.47.2.224
- Merkulov, G. A. (1969). Course of pathohistological techniques. Moscow: Medicine, 424.
- Pierce, E. (1962). Histochemistry. Moscow: Foreign Literature, 967.
- Lyapunov, N. A., Zagora, V. A., Georgeevsky, V. P., Bezugly, E. P. (Eds.) (1999). Good manufacturing practices of medicines. Kyiv: Morion, 896.
- Nimaeva, D. E., Sizykh, T. P. (2002). The nature of liver disease in patients with diabetes mellitus type 2. Siberian Journal of Medicine, 35 (6), 14–21.
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