The application of arbutin in elimination resistance gramm-negative multidrug resistance bacteria of Pseudomonas aeruginosa and Enterobacter cloacea

Authors

DOI:

https://doi.org/10.15587/2519-4852.2024.305965

Keywords:

arbutin, multi-drug resistant, Gram-negative strains, molecular docking, removal resistance, antibiotics

Abstract

The basic therapy against microbial infections is the application of antibiotics. However, overuse of antibiotics has become the major factor for the emergence and dissemination of multidrug-resistant strains.

The aim. The purpose of our work was to investigate the in vitro and in silico elimination resistance of antibiotics (сlarithromycin, azithromycin, gentamycin, ciprofloxacin, levofloxacin, ceftriaxone, and chloramphenicol) against clinical multidrug-resistant strains of P. aeruginosa and E. cloacae by arbutin.

Materials and methods. The molecular docking was performed using AutoDockTools 1.5.6; antimicrobial effects were evaluated by the well method. Isolates were obtained from clinical samples including tracheal aspirate and broncoalveolar lavage.

Results. Theoretical studies have found that none of the investigated antibiotics and arbutin highly selectively inhibit all "targets" mechanisms of antimicrobial action. In experimental studies, it was observed that adding arbutin to the antibiotic led to the emergence of sensitivity on the part of resistant strains. All Gramm-negative resistance strains of bacteria were sensitive to the action of arbutin. Moreover, arbutin increased the antimicrobial effect of antibiotics from 8 to 55 %. It was estimated exceptions such as clarithromycin and azithromycin when assessing antimicrobial activity against P. aeruginosa.

Conclusions. These studies have shown that inhibiting resistant strains of bacteria requires the use of combinations of "classical" antimicrobials and herbal drugs or dietary supplements based on extracts obtained from arbutin-containing medicinal plants such as lingonberry, bearberry, and cranberry. This approach is a “lifeline” for the development of antimicrobial agents against resistant bacteria and gives “a second chance to return to life” for outdated antibiotics

Supporting Agency

  • Ministry of Health of Ukraine, carried out at the expense of the state budget of Ukraine No. 0124U002080 (Order of the Ministry of Health of Ukraine No. 17 of 27.02.2024). We are grateful for the scientific and material help provided by the pharmaceutical company "Astrapharm" Kyiv, Ukraine, and the pharmaceutical company "Zdravopharm", Kharkiv, Ukraine.

Author Biographies

Oleksandr Maslov, National University of Pharmacy

PhD

Department of General Chemistry

Mykola Komisarenko , National University of Pharmacy

PhD

Department of Pharmacognosy and Nutriciology

Svitlana Ponomarenko, Mechnikov Institute of Microbiology and Mechnikov Institute of Microbiology and Immunology of the NAMS of Ukraineof the NAMS of Ukraine

PhD

Laboratory of Biochemistry and Biotechnology

Tetiana Osolodchenko, Mechnikov Institute of Microbiology and Immunology of the NAMS of Ukraine

PhD

Laboratory of Biochemistry and Biotechnology

Sergii Kolisnyk , National University of Pharmacy

Doctor of Pharmaceutical Science

Department of General Chemistry

Oleh Koshovyi, National University of Pharmacy; University of Tartu

Doctor of Pharmaceutical Sciences, Professor

Department of Pharmacognosy;

Doctor of Pharmaceutical Science

Institute of Pharmacy

Andrey Komissarenko , Pharmaceutical Company "ZDRAVOPHARM"

Doctor of Pharmaceutical Science

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The application of arbutin in elimination resistance gramm-negative multidrug resistance bacteria of Pseudomonas aeruginosa and Enterobacter cloacea

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Published

2024-10-22

How to Cite

Maslov, O. ., Komisarenko , M., Ponomarenko, S. ., Osolodchenko, T. ., Kolisnyk , S. ., Koshovyi, O., & Komissarenko , A. (2024). The application of arbutin in elimination resistance gramm-negative multidrug resistance bacteria of Pseudomonas aeruginosa and Enterobacter cloacea. ScienceRise: Pharmaceutical Science, (5 (51), 38–46. https://doi.org/10.15587/2519-4852.2024.305965

Issue

No. 5 (51) (2024)

Section

Pharmaceutical Science

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Copyright (c) 2024 Oleksandr Maslov, Mykola Komisarenko , Svitlana Ponomarenko, Tetiana Osolodchenko, Sergii Kolisnyk , Oleh Koshovyi, Andrey Komissarenko

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