Scientific and experimental substantiation of the technology for antigens of candida fungi purification

Authors

DOI:

https://doi.org/10.15587/2519-4852.2017.93905

Keywords:

antigen, vaccine, candidiasis, technology, filtration, ultrafiltration, centrifugation, therapy, prevention, immunity

Abstract

Every year, the number of patients with candidiasis increases. Many researchers believe that the creation of vaccines against candidiasis is a promising direction in the fight against this disease. The authors substantiated the technology of C. albicans and C. tropicalis fungi cells disintegration to produce antigens.

The aim of this work was to substantiate the technology C. albicans and C. tropicalis fungi antigens purification from ballast substances.

Materials and methods. First and foremost, it was necessary to separate unbroken cells from destroyed cells remnants. Then, purification from shallow mechanical residues in solution containing fungi antigens was necessary. To prevent possible contamination of equipment and personnel, sterilization of the antigens solution became important. The use of ultrafiltration and gel chromatography was analyzed for separation of substances by molecular weight analysis. Determination of activity of the obtained fungi antigens fractions in prevention and treatment of candidiasis was carried out experimentally in mice.

Results. According to the research results, the technology of purification of fungi antigens, consisting of sequential processes: centrifugation, previous, sterilizing and ultrafiltration, was developed.

Conclusion. Our studies in mice suggest that the developed technology of antigens C. albicans and C. tropicalis purification provides the necessary result in candidiasis prevention and treatment

Author Biographies

Mykola Rybalkin, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

PhD, Assistant

Department of biotechnology

Leonid Strelnikov, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Doctor of Pharmaceutical Sciences, Professor

Department of biotechnology

References

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Published

2017-02-28

How to Cite

Rybalkin, M., & Strelnikov, L. (2017). Scientific and experimental substantiation of the technology for antigens of candida fungi purification. ScienceRise: Pharmaceutical Science, (1 (5), 62–65. https://doi.org/10.15587/2519-4852.2017.93905

Issue

No. 1 (5) (2017)

Section

Pharmaceutical Science

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Copyright (c) 2017 Микола Вікторович Рибалкін, Леонід Семенович Стрельников

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