DOI: https://doi.org/10.15587/2519-4852.2017.113503

New possibilities of use of oat raw materials for investigation of biologically active components in therapy of experimental diabetes

Lyudmila Gayova, Nataliya Yurzhenko, Olga Mysnik

Abstract


Aim of the work is to suggest the technology of aqua dynamic extraction of bioactive substances from oat straw. The amount of the extract and the concentration of extracted substances depend on the duration of extraction process. The composition of the investigated phytocomplex is established by nuclear magnetic resonance. Fatty acids composition of the phytocomplex of the oat straw extract was studied by gas chromatography. The possibility of using a phytocomplex from an oat straw extract is shown in the study of the metabolism of myocardium lipids in experimental animals with a diabetes model.

Methods of research: to optimise the process of aqueous extraction of the oat straw that in prospect, with the help of dynamic character and increased temperatures, can prevail obvious disadvantages of technology offered before; in order to evaluate the differences in the content of the mixtures of the substances, received from the primary extract was noted observation Н1–NMR spectra in D2O; was performed gas chromatographic analysis of the fatty acids spectrum in the studied phytocomplex and in the lipids of the white blood cells of the myocardium; modeling of diabetes was performed by intraperitoneal streptozotocin injection at dose of 55 mg per kilo of body weight of rats during 6 weeks.

Results: was developed more economically expedient approach to obtain a phytocomplex by extracting oat straw with full preservation of biologically active substances. Fatty acid composition of the phytocomplex of the oat straw extract was studied by gas chromatography. Composition of the phytocomplex of the oat straw extract was studied by nuclear magnetic resonance

Conclusions: The possibility of using a phytocomplex from an oat straw extract in the study of the metabolism of myocardium lipids in experimental animals with a model of diabetes is shown

Keywords


Avena Sativa; straw; extraction; polyphenols; antioxidants; fatty acids; experimental diabetes

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References


Avar, P., Nikfardjam, M. S. P., Kunsági-Máté, S., Montskó, G., Szabó, Z., Böddi, K. et. al. (2007). Investigation of Phenolic Components of Hungarian Wines. International Journal of Molecular Sciences, 8 (10), 1028–1038. doi: 10.3390/i8101028

Kushwaha, S. C., Bera, M. B., Kumar, P. (2013). Nutritional composition of detanninated and fresh pomergranate peel powder. IOSR Journal Of Environmental Science, Toxicology And Food Technology, 7 (1), 38–42. doi: 10.9790/2402-0713842

López-Martínez, L. M., Santacruz-Ortega, H., Navarro, R.-E., Sotelo-Mundo, R. R., González-Aguilar, G. A. (2015). A 1H NMR Investigation of the Interaction between Phenolic Acids Found in Mango (Manguifera indica cv Ataulfo) and Papaya (Carica papaya cv Maradol) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) Free Radicals. PLOS ONE, 10 (11), e0140242. doi: 10.1371/journal.pone.0140242

Erdem, F., Cetinkaya, N., Nisbet, C., Altin, E. (2016). Estimation of organic matter digestibility, metabolizable energy, phenolic compounds and antioxidant activity of stems and seeds of the Juncus acutus plant in ruminant nutrition. South African Journal of Animal Science, 45 (5), 502. doi: 10.4314/sajas.v45i5.7

Shadi, T. Z., Talal, A. Z. (2015). A review of four common medicinal plants used to treat eczema. Journal of Medicinal Plants Research, 9 (24), 702–711. doi: 10.5897/jmpr2015.5831

Kushnerova, N. F., Fomenko, S. Ye., Sprygin, V. H., Kushnerova, T. V., Lesnikova, L. N. (2009). Plant complexes of polyphenols in the recovery of liver functions in patients with alcoholism. Health. Medical ecology. Science, 4-5 (39-40), 95–97.

Shamrai, Ye. F. (1952). Chemical interaction and biological interconnection of ascorbic acid and some polyphenols. Kyiv, 19.

Shamrai, Ye. F. (1953). Pat. No. 105777. Method of preparation of the medicine Galaskorbinum. No. 2713/454356; declareted: 28.10.1953.

Shamrai, Ye. F. (1959). Pat. No. 130415. Contraceptive pills. No. 645652/31; declareted: 01.12.1959, Bul. No. 14.

Kraidashenko, O. V., Berezin, O. Ye. (1997). Pat. No. 18108 UA. Method for treatment of chronic ischemic heart disease in elderly and old age patients. MPK: A61K 31/015, A61P 9/10, A61K 31/185. declareted: 01.07.1997.

Shamrai, Ye. F., Dziubko, N. Ya. (1968). Recommendations for the use of galascorbinum in radiation therapy of cancer patients. Kyiv.

Naumova, H. M. (1975). Comparative biological activity of galascorbinum and askopol in radiolesions of the body (experimental studies). Kyiv, 25.

Mitrofanov, R. Yu., Zolotukhin, V. N., Budaieva, V. V. (2010). Study of chemical composition of aqueous extract of oat straw (Avena Sativa L.) and study of its growth regulating properties. Polzunovskyi vestnik, 1 (4), 174–179.

Mushtaq, A., Gul-Zaffar, Dar, A., Mehfuza, H. (2014). A review on Oat (Avena sativa L.) as a dual-purpose crop. Scientific Research and Essays, 9 (4), 52–59. doi: 10.5897/sre2014.5820

Ponomariova, A. H., Medvedev, V. M. (1998). Pat. No. 2145864 RU. Phytocomplex for prevention and treatment of diseases of different etiology. MKP A61K35/78. No. 98123970/14; declareted: 30.12.1998; published: 27.02.2000.

Linnikova, V. A., Linnikov, A. M. (2004). Pat. No. 2282459 RU. Tincture of Avena Sativa Z. of milk-wax stage of ripeness, medicine composed Avena Sativa Z. of milk-wax stage of ripeness, method of preparation of tincture of Avena Sativa Z. of milk-wax stage of ripeness. MKP A61K36/899. No. 2004123801/15; declareted: 03.08.2004; published: 27.08.2006.

Pat. No. 2125462S1 RU. Tincture of Avena Sativa Z. of milk stage, medicines composed of Avena Sativa Z. of milk stage and methods of its preparation. MKP A61K35/78 (1998). No. 98101233/14; declareted: 21.01.1998; published: 27.01.1999.

Yaremenko, O. B., Kamysh, O. Yu., Briuzina, T. S. (2005). Otsinka zhyrnokyslotnoho skladu lipidiv krovi u khvorykh na revmatoidnyi artryt. Medychna khimiya, 2, 86–88.

Gorlova, O. S. (2016). Ekologiya i farmakologicheskiye svoystva Vakhty trekhlistnoy (menyanthes trifoliata l.). Uchenyye zapiski UO VGAVM, 52 (3), 30–32.


GOST Style Citations


Avar, P. Investigation of Phenolic Components of Hungarian Wines [Text] / P. Avar, M. S. P. Nikfardjam, S. Kunsági-Máté, G. Montskó, Z. Szabó, K. Böddi et. al. // International Journal of Molecular Sciences. – 2007. – Vol. 8, Issue 10. – P. 1028–1038. doi: 10.3390/i8101028 

Kushwaha, S. C. Nutritional composition of detanninated and fresh pomergranate peel powder [Text] / S. C. Kushwaha, M. B. Bera, P. Kumar // IOSR Journal Of Environmental Science, Toxicology And Food Technology. – 2013. – Vol. 7, Issue 1. – P. 38–42. doi: 10.9790/2402-0713842 

López-Martínez, L. M. A 1H NMR Investigation of the Interaction between Phenolic Acids Found in Mango (Manguifera indica cv Ataulfo) and Papaya (Carica papaya cv Maradol) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) Free Radicals [Text] / L. M. López-Martínez, H. Santacruz-Ortega, R.-E. Navarro, R. R. Sotelo-Mundo, G. A. González-Aguilar // PLOS ONE. – 2015. – Vol. 10, Issue 11. – P. e0140242. doi: 10.1371/journal.pone.0140242 

Erdem, F. Estimation of organic matter digestibility, metabolizable energy, phenolic compounds and antioxidant activity of stems and seeds of the Juncus acutus plant in ruminant nutrition [Text] / F. Erdem, N. Cetinkaya, C. Nisbet, E. Altin // South African Journal of Animal Science. – 2016. – Vol. 45, Issue 5. – P. 502. doi: 10.4314/sajas.v45i5.7 

Shadi, T. Z. A review of four common medicinal plants used to treat eczema [Text] / T. Z. Shadi, A. Z. Talal // Journal of Medicinal Plants Research. – 2015. – Vol. 9, Issue 24. – P. 702–711. doi: 10.5897/jmpr2015.5831 

Kushnerova, N. F. Plant complexes of polyphenols in the recovery of liver functions in patients with alcoholism [Text] / N. F. Kushnerova, S. Ye. Fomenko, V. H. Sprygin, T. V. Kushnerova, L. N. Lesnikova // Health. Medical ecology. Science. – 2009. – Issue 4-5 (39-40). – P. 95–97.

Shamrai, Ye. F. Chemical interaction and biological interconnection of ascorbic acid and some polyphenols [Text]: abstract of Dr. Sci. (Biol.) dissertation / Ye. F. Shamrai. – Kyiv, 1952. – 19 p.

Pat. No. 105777. Method of preparation of the medicine Galaskorbinum [Text] / Shamrai Ye. F. – No. 2713/454356; declareted: 28.10.1953.

Pat. No. 130415. Contraceptive pills [Text] / Shamrai Ye. F. – No. 645652/31; declareted: 01.12.1959, Bul. No. 14.

Pat. No. 18108 UA. Method for treatment of chronic ischemic heart disease in elderly and old age patients. MPK: A61K 31/015, A61P 9/10, A61K 31/185 [Text] / Kraidashenko O. V., Berezin O. Ye. – declareted: 01.07.1997.

Shamrai, Ye. F. Recommendations for the use of galascorbinum in radiation therapy of cancer patients [Text] / Ye. F. Shamrai, N. Ya. Dziubko. – Kyiv, 1968.

Naumova, H. M. Comparative biological activity of galascorbinum and askopol in radiolesions of the body (experimental studies) [Text]: abstract of Dr. Sci. (Med.) dissertation / H. M. Naumova. – Kyiv, 1975. – 25 p.

Mitrofanov, R. Yu. Study of chemical composition of aqueous extract of oat straw (Avena Sativa L.) and study of its growth regulating properties [Text] / R. Yu. Mitrofanov, V. N. Zolotukhin, V. V. Budaieva // Polzunovskyi vestnik. – 2010. – Vol. 1, Issue 4. – P. 174–179.

Mushtaq, A. A review on Oat (Avena sativa L.) as a dual-purpose crop [Text] / A. Mushtaq, Gul-Zaffar, A. Dar, H. Mehfuza // Scientific Research and Essays. – 2014. – Vol. 9, Issue 4. – P. 52–59. doi: 10.5897/sre2014.5820 

Pat. No. 2145864 RU. Phytocomplex for prevention and treatment of diseases of different etiology. MKP A61K35/78 [Text] / Ponomariova A. H., Medvedev V. M. – No. 98123970/14; declareted: 30.12.1998; published: 27.02.2000.

Pat. No. 2282459 RU. Tincture of Avena Sativa Z. of milk-wax stage of ripeness, medicine composed Avena Sativa Z. of milk-wax stage of ripeness, method of preparation of tincture of Avena Sativa Z. of milk-wax stage of ripeness. MKP A61K36/899 [Text] / Linnikova V. A., Linnikov A. M. – No. 2004123801/15; declareted: 03.08.2004; published: 27.08.2006.

Pat. No. 2125462S1 RU. Tincture of Avena Sativa Z. of milk stage, medicines composed of Avena Sativa Z. of milk stage and methods of its preparation. MKP A61K35/78 [Text] / Open joint-stock company Pharmaceutical factory of St. Petersburg. – No. 98101233/14; declareted: 21.01.1998; published: 27.01.1999.

Yaremenko, O. B. Otsinka zhyrnokyslotnoho skladu lipidiv krovi u khvorykh na revmatoidnyi artryt [Text] / O. B. Yaremenko, O. Yu. Kamysh, T. S. Briuzina // Medychna khimiya. – 2005. – Issue 2. – P. 86–88.

Gorlova, O. S. Ekologiya i farmakologicheskiye svoystva Vakhty trekhlistnoy (menyanthes trifoliata l.) [Text] / O. S. Gorlova // Uchenyye zapiski UO VGAVM. – 2016. – Vol. 52, Issue 3. – P. 30–32.







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