New possibilities of use of oat raw materials for investigation of biologically active components in therapy of experimental diabetes

Authors

DOI:

https://doi.org/10.15587/2519-4852.2017.113503

Keywords:

Avena Sativa, straw, extraction, polyphenols, antioxidants, fatty acids, experimental diabetes

Abstract

Aim of the work is to suggest the technology of aqua dynamic extraction of bioactive substances from oat straw. The amount of the extract and the concentration of extracted substances depend on the duration of extraction process. The composition of the investigated phytocomplex is established by nuclear magnetic resonance. Fatty acids composition of the phytocomplex of the oat straw extract was studied by gas chromatography. The possibility of using a phytocomplex from an oat straw extract is shown in the study of the metabolism of myocardium lipids in experimental animals with a diabetes model.

Methods of research: to optimise the process of aqueous extraction of the oat straw that in prospect, with the help of dynamic character and increased temperatures, can prevail obvious disadvantages of technology offered before; in order to evaluate the differences in the content of the mixtures of the substances, received from the primary extract was noted observation Н1–NMR spectra in D2O; was performed gas chromatographic analysis of the fatty acids spectrum in the studied phytocomplex and in the lipids of the white blood cells of the myocardium; modeling of diabetes was performed by intraperitoneal streptozotocin injection at dose of 55 mg per kilo of body weight of rats during 6 weeks.

Results: was developed more economically expedient approach to obtain a phytocomplex by extracting oat straw with full preservation of biologically active substances. Fatty acid composition of the phytocomplex of the oat straw extract was studied by gas chromatography. Composition of the phytocomplex of the oat straw extract was studied by nuclear magnetic resonance

Conclusions: The possibility of using a phytocomplex from an oat straw extract in the study of the metabolism of myocardium lipids in experimental animals with a model of diabetes is shown

Author Biographies

Lyudmila Gayova, Bogomolets National Medical University T. Shevchenko blvd., 13, Kyiv, Ukraine, 01601

MD, Professor

Department of Bioorganic and Biological Chemistry

Nataliya Yurzhenko, Bogomolets National Medical University T. Shevchenko blvd., 13, Kyiv, Ukraine, 01601

PhD, Senior Lecturer

Department of Bioorganic and Biological Chemistry

Olga Mysnik, Bogomolets National Medical University T. Shevchenko blvd., 13, Kyiv, Ukraine, 01601

PhD, Associate Professor

Department of Bioorganic and Biological Chemistry

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Published

2017-10-31

How to Cite

Gayova, L., Yurzhenko, N., & Mysnik, O. (2017). New possibilities of use of oat raw materials for investigation of biologically active components in therapy of experimental diabetes. ScienceRise: Pharmaceutical Science, (5 (9), 14–20. https://doi.org/10.15587/2519-4852.2017.113503

Issue

No. 5 (9) (2017)

Section

Pharmaceutical Science

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Copyright (c) 2017 Lyudmila Gayova, Nataliya Yurzhenko, Olga Mysnik, Mihaylo Rodyhin

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