DOI: https://doi.org/10.15587/2519-4852.2018.135748

Influence of extract of peach ordinary (persica vulgaris) leaves on the state of thymic-lymphatic element of the immune system of rats in conditions of chronic immobilization stress

Oksana Mishchenko, Ganna Zaychenko, Churshed Sharifov, Оlena Koshova, Julia Larianovskaya, Olena Khalieieva

Abstract


The aim – study of the influence of thick extract of peach ordinary (Persica vulgaris) leaves (TEPL) on the state of thymic-lymphatic element of the immune system of rats in conditions of chronic immobilization stress.

Materials and methods. The model of chronic immobilization stress was reproduced for 18 days by daily four hour immobilization of rats in tight box. Investigated TEPL, which was obtained at the Department of Chemistry of Natural Compounds of National University of Pharmacy from peach leaves of the Salve variety harvested in Tajikistan was administered intragastrically in a conditionally effective dose of 100 mg/kg. Reference drug the syrup «Immuno-Ton» was used in a dose of 3 ml/kg. The state of thymic-lymphatic element of the immune system in conditions of chronic immobilization stress was determined after the euthanasia of animals under mild inhalation anesthesia by the results of the study of the coefficients of thymus and spleen mass and histological studies on micropreparations, which were prepared according to the generally accepted method. To assess the nature of the effect of the TEPL on the condition of the organs of the rat thymic-lymphatic system under chronic immobilization stress conditions, a comparison was made with intact control and control pathology.

Results. It was determined that chronic immobilization stress had suppressive effect on the thymic-lymphatic element of the immune system. An increase in the width of the marginal zone of lymphoid follicles and muft and the number of perivascular lymphatic clusters 1.2 times (p <0.05) compared with control pathology, reduction of signs of stressful hypoxia - the number of structures of white pulp with spasm of the central arteries was noted in the spleen. Reducing the degree of change from the third to fourth phase to the first phase of the accident transformation is set in the thymus, which is confirmed by an increase in the mass of the thymus mass by 2.7% in comparison with the control pathology.

Conclusions. The ability of TEPL to restore impaired activity of organs of thymic-lymphatic element of the immune system has been proved. It is likely, that polyphenolic compounds of TEPL exhibit antioxidant properties, enhancing the activity of the antioxidant system, and polysaccharides exhibit immunostimulative properties, the result of which is a stressprotective action. The effectiveness of TEPL was not inferior to the reference drug «Immuno-Ton»


Keywords


thick extract peach ordinary leaves; chronic immobilization stress; thymus; spleen; stress-protective effect

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References


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GOST Style Citations


Kirichek L. T., Perepelitsa A. V., Kal'chuk R. O. Lekarstvennyy antistress v eksperimente. Kharkіv: IPP «Kontrast», 2015. 104 p.

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Kurkin V. A., Poroykov V. V. Fenilpropanoidy lekarstvennykh rasteniy: prognoz antioksidantnoy i immunomoduliruyushhey aktivnosti // Sovremennye problemy nauki i obrazovaniya. 2015. Issue 2 (2). URL: https://www.science-education.ru/ru/article/view?id=22694

Lipkan G. N. Lekarstvennye rasteniya – adaptogeny. Kyiv, 2014. 686 p.

Fitokhimicheskoe obosnovanie farmakologicheskikh effektov fenol'nykh soedineniy persika obyknovennogo / Zaychenko A. V. et. al. // Fіtoterapіya. Chasopis. 2014. No. 4. P. 71–74.

Influence of peach leaf extract on the metabolic processes and prooxidant/antioxidant balance in rats in conditions of chronic immobilization stress / Zaychenko G. et. al. // ScienceRise: Pharmaceutical Science. 2017. Vol. 1, Issue 5. P. 13–16. doi: http://doi.org/10.15587/2519-4852.2017.92521 

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