Phytochemical and pharmacological study of polysaccharide complexes of prunus domestica fruit

Authors

  • Taras Upyr National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002, Ukraine https://orcid.org/0000-0003-2374-5497
  • Shahm Basim Mohammed National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002, Ukraine
  • Al-Jabbar Ali Sahlani Bashar National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002, Ukraine
  • Larysa Lenchyk National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002, Ukraine https://orcid.org/0000-0002-6551-9036
  • Igor Senyuk National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002, Ukraine
  • Viktoria Kyslychenko National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002, Ukraine https://orcid.org/0000-0002-0851-209X

DOI:

https://doi.org/10.15587/2519-4852.2018.135825

Keywords:

plum, extract, polysaccharides, elements, histostructure, rats liver, ethanol intoxication

Abstract

Aim. The aim of our research was fractionation of polysaccharide complexes from fresh plum fruits, comparative study content of neutral sugars in it, element composition of plum fruit and investigation of influence of fibers and water soluble polysaccharide complex (WSPC) on histostructure of rats liver in conditions of ethanol intoxication

Methods. The elemental composition was determined with atomic emission spectrophotometer ISE 3500 (Thermo Scientific, USA). The content of polysaccharide fractions as fibers, WSPC and pectin was determined by Gravimetry. The comparative study of monosaccharides content in obtained fractions was carried out with picric acid method on a Hewlett Packard 8453 spectrophotometer at a wavelength of 463 nm in a cuvette with a layer thickness of 10 mm.

Investigation of the influence of fibers and water soluble polysaccharide complex (WSPC) of plum fruit on histostructure of rat liver was carried out on the model of ethanol intoxication.

Results. As a result of the study element composition were determined. Potassium has the most content in fruits– 2000 μg/100 g. Content of heavy metals in the plum fruit does not exceed the norms established by the State Pharmacopoeia of Ukraine. Three polysaccharide fractions, as fibers WSPC and pectines, were obtained from the fresh plum fruits. The most content of neutral sugars were determined in the WSPS – 61.52±1.47 %. Their content in other complexes was: in fibers – 59.23±1.15 %, pectin – 22.85±0.55 %.

It was confirmed that simultaneous administration of alcohol with the fibers of plum fruits in a dose of 200 mg / kg prevents the development of fatty dystrophy of the liver parenchyma, in contrast to the similar scheme of the administration of the WSPC of plum fruit in the same dose or drug comparison silibor at a dose of 30 mg/kg.

Conclusions. Considering results of phitochemical and pharmacological research we can assume that the plum fruit fiber is promising for further study in order to create a new effective and safe drugs for use in medical practice

Author Biographies

Taras Upyr, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Аssistant

Department of Pharmacology

Shahm Basim Mohammed, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Postgraduate Student

Department of Chemistry of Natural Compounds

Al-Jabbar Ali Sahlani Bashar, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Postgraduate Student

Department of Biological Chemistry

Larysa Lenchyk, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Doctor of Pharmaceutical Sciences, Associate Professor

Department of Chemistry of Natural Compounds

Igor Senyuk, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

PhD, Associate Professor

Department of Biological Chemistry

Viktoria Kyslychenko, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Doctor of Pharmaceutical Sciences, Professor

Department of Chemistry of Natural Compounds

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Published

2018-06-29

How to Cite

Upyr, T., Basim Mohammed, S., Bashar, A.-J. A. S., Lenchyk, L., Senyuk, I., & Kyslychenko, V. (2018). Phytochemical and pharmacological study of polysaccharide complexes of prunus domestica fruit. ScienceRise: Pharmaceutical Science, (3 (13), 32–37. https://doi.org/10.15587/2519-4852.2018.135825

Issue

Section

Pharmaceutical Science