Quantitative determination of microbiom in the destination content the gut in rats

Abstract

Introduction. The gut microbiome significantly affects the functioning of the body: it participates in the protection of the body against pathogenic microorganisms, in the processes of metabolism, inhibition of inflammatory responses, in the formation of innate and adaptive immune response in the intestinal mucosa. One of the reasons for changing the microbiota is the use of antibiotics. Therefore, the processes of interaction of antibiotics, Salmonella enteritidis and Salmonella typhimurium with representatives of normal intestinal microflora are of particular interest. Materials and methods. The quantitative and qualitative composition of the wall microbiota in rats by bacteriological method, the statistical analysis of data using the program StatSoft Statistica v12 were conducted. Results & discussion. With the introduction of vancomycin and S. enteritidis, S. typhimurium in groups II, III, IV there was a decrease in E. coli levels by 10, 7 and 110 times, respectively (p≤0.05). The number of P. aeruginosa decreased significantly only in the third group (p≤0.05). The number of representatives of Bacteroides spp. significantly decreased by several thousand times (group II) and by 70 and 87 times (groups III and IV) (p≤0.05). The content of E. faecalis and E. faecium decreased by 861,6 and several thousand times (group II, III, IV) (p≤0.05). The number of Proteus spp. significantly decreased in group II by 27 times and increased rapidly in group IV (p≤0.05). Group III showed a sharp decrease in the content of representatives of Enterobacter spp. and Klebsiella spp. in 847 and 150 times, and in group II there is an increase in their number by 7 and 46 times, respectively (p≤0.05). The quantitative composition of Peptostreptococcus anaerobius decreased significantly in all three study groups (p≤0.05). The level of Salmonella spp. increased in group II by 49 times and significant increase was observed in groups III and IV (p≤0.05). An intense increase in the number of Shigella spp. is noted in all three groups. The introduction of salmonella, against the background of vancomycin pre-treatment, causes a dramatic change in the composition of the microbiota in groups V and VI, namely: an increase in the number of E. coli 65 and 105 times, a significant increase in the level of P. aeruginosa in the fifth group, and in the sixth, 3 times. Also, in groups there is a decrease in the number of Bacteroides spp. 9 and 10 times (p≤0.05). The content of E. faecalis and E. faecium decreased significantly only in the fifth group (p≤0.05). The number of Proteus spp. decreases 17 times in group V and also a significant decrease was observed in group VI (p≤0.05). A sharp increase in the content of representatives of Enterobacter spp. and Klebsiella spp. was observed in the fifth and sixth groups (p≤0.05). However, representatives of Peptostreptococcus anaerobius in V and VI groups decreased 20 and 9 times, respectively (p≤0.05). The number of Salmonella spp. decreased only in group V 7 times (p≤0,05). There was also a decrease in the number of Shigella spp. 538 and 860 times, respectively (p≤0.05). With the introduction of experimental animals B. fragilis treated with S. enteritidis, S. typhimurium on the background of vancomycin pre-treatment, a significant decrease in the level of E. coli in group VII, and in VIII - by 538 times (p≤0.05). The number of P. aeruginosa in groups VII and VIII decreased significantly and the number of representatives of Bacteroides spp. naturally increases (p≤0.05). The content of E. faecalis and E. faecium increased by 10 and 19 times in the seventh and eighth groups respectively, and the number of Proteus spp. decreases only in Group VII 322 times (p ≤0.05). Also, in VII and VIII groups there is a sharp decrease in the content of representatives of Enterobacter spp. and Klebsiella spp. (p≤0.05). The level of representatives of Peptostreptococcus anaerobius increased significantly 7 and 12 times (groups VII and VIII, respectively) (p≤0.05). The number of S. enteritidis and S. typhimurium in the VII and VIII groups decreased intensively with less pronounced decrease in the level of Shigella spp. (p≤0.05). Conclusions. The introduction of B. fragilis can be used in the treatment of inflammatory bowel diseases or diseases with impaired barrier function of the intestine.