The pharmacological efficacy of the extemporaneous gel Diavenol

Abstract

Introduction. Diavenol - new extemporaneous gel for the treatment of venous ulcers. The gel contains a synergistic combination C. majus quinolizidine alkaloids and riboflavin. Activate tissue regeneration shown previously for riboflavin, and сheleretrin / sanguirythrine. The combination of the alkaloids and riboflavin pharmacologically fully justified. The research aim was to check the mechanism action of the Diavenol gel - through stimulation of  the pluripotent cells growth and determination the effectiveness of the gel for wound healing at experimental model in rats. Materials and methods. In a study 128 rats were used, of which 38 - in the pharmacological experiment with a stencil wound healing, and 90 in the experiment to study the influence of gel on CD34 cells amount in the blood in rats with chemically induced immunodeficiency. High immunodeficiency caused a five-fold administration of cyclophosphamide, prednisone and rubomycin. CD34 expression level was determined using flow cytometry FACS Calibur. Results and discussion. Topical skin application the Diavenol gel samples in rats helped to accelerate the regeneration process is about 2 times, as evidenced by the excess of blood parameters in experimental group animals to the 30th day, more than 2 times, while the indicators in the control group animals came to normal values only on the 60th day. Increasing the number of pluripotent cells as well as acceleration of wound regeneration indicates preferential stimulation of stem cell growth in the periphery, rather than an enhancement of bone marrow function. The maximum effect of the Diavenol observed on the 60th day and the restoration of the pluripotent cells physiological level have been observed on the 10th day after the start of Diavenol application. Due to the fact, that the gel was applied to intact skin, we can say that Diavenol had resorptive properties. Treatment of wounds in rats Diavenol gel resulted in a more rapid removal of inflammation and significant acceleration of stencil wound healing. In terms of the granulation appearance and wounds epithelialization the Diavenol gel has some advantage in relation to the dexpanthenol ointment.