The study antimicrobial activity of combination drugs on the basis of hydrogen peroxide and aethonium

Abstract

Formulation of two complex antimicrobial compositions on the basis of hydrogen peroxide and aethonium has been developed: hydrogen peroxide – 16.7 %; aethonium – 12.0 %; sodium pyrophosphate – 0.06 %; water and stabilizing agents up to 100 % (composition 1) and hydrogen peroxide – 18.0 %; aethonium – 12.0 %; ethanol – 12,5 %; water and stabilizings agent up to 100 % (composition 2); and their antimicrobial activity has been studied by agar diffusion method on standard microorganism test strains:Staphylococcusaureus ATCC25923;StaphylococcusaureusATCC6538;EscherichiacoliATCC25922;EscherichiacoliATCC11229;Pseudomonas aeruginosaATCC27953;Pseudomonas aeruginosaATCC15442;ProteusvulgarisH4636;ProteusmirabilisATCC14153; EnterococcusfaeciumATCC6057;Bacillus cereus  ATCC 10702; Candida albicans ATCC 10231.We have carried out assessment of integrated antimicrobial activity of complex drugs using vector algebra. It has been demonstrated that there is a direct correlation between the integrated index of antimicrobial activity and logarithm of concentration of antimicrobial components in the solution. An advanced mathematical model for description of the dependence of activity spectrum on concentration of antimicrobial drugs has been suggested – logistic dependence.It has been determined that composition 1 is 13.5 % more active when compared to composition 2. In the result of the study of antiseptic compositions 1 and 2 in the following dilutions: undiluted, 1:2, 1:4, 1:8, 1:16, and determination of their microbicidal activity associated with 1-3-5-10-30-60-minute exposition with microbial burden of clinical and test strains 2·10⁹ CFU/mL using the method for assessment of antimicrobial activity of drugs developed, we have come to the conclusion that antimicrobial properties of the compositions studied maintain at the high level up to their dilution to 1:4; at this dilution, an optimal bactericidal exposition of 10 min has been determined.We have come to the conclusion as for potential of use of the combined antimicrobial compositions on the basis of hydrogen peroxide and quaternary ammonium compounds suggested as antiseptic agents and disinfectants in medicine and/or veterinary.