Study of antimicrobial activity of new combined ointment on the model of purulent wound of staphylococcal etiology

Abstract

Introduction. Prevalence of purulent pathologies and insufficient number of combined ointments with multi-effect on inflammation, infection and tissue damage are a prerequisite for scientists of NUPh to develop a new combined ointment composed of: antiseptics ethacridine lactate and thick chlorophyllipt extract, drug with reparative and anti-inflammatory activity – dexpanthenol, PEG-base of the IInd generation with the moderate dehydrating activity (180%). The range of pharmacological actions of Filetol ointment meets local therapy requirements in the IInd phase of the wound healing process. New ointment can also be applied in the Ist phase of wound healing process, if the wound contains a small amount of purulent exudate or does not contain it at all.

Material & Methods. Thepurulentwoundofstaphylococcal etiology was reproduced in 24 white mature female mice weighing 14-17 g and subcutaneously injected with 0.2 ml of 10% solution of calcium chloride in the skin area, depilated beforehand, and after the development of necrosis on the 4th day injected with 0.2 ml of daily agar cultures S. aureus ATCC 25923 in the necrotizing area. In 48 hours of the infection, in the area of administration of staphylococcus cultures purulent necrotic wounds developed, which, depending on the size and intensity of the lesions, were subjected to reverse development in 9-15 days in a row. Animals were divided into three groups of 8 animals, including a positive control group, animals in which were not treated. In this study, the comparator was Levomecol ointment which is widely used in clinical practice for the local treatment of purulent wounds. Efficacy of treatment was evaluated on the basis of lesion value and intensity in study groups compared with the positive control, animal deaths in the case of generalization of the purulent process, quantitative dynamics of microorganisms in the wound and planimetric indicators (area of wounds and the percentage of rats with healed wounds).

Results & Discussion. Intradermal administration of staphylococcus to mice resulted in the development of severe purulent necrotic wounds in animals which in the positive control group was accompanied by increase in titer of microbial contamination during the experiment. Due to the generalization of infection, high mortality of animals was observed and in the positive control group it amounted to 50%.

Application of Filetol ointment contributed to the rapid cleansing of wounds from purulent necrotic masses, reducing the number of microorganisms and activation of reparative processes. From the 3rd day of treatment with Filetol ointment, content of S. aureus in the wound decreased by half and was 2х10³-3х10⁴. Full liberation of wounds of Staphylococcus in the treatment with Filetol ointment was observed on the 6^th day of treatment. In animals treated with Levomecol ointment, liberation of wounds of Staphylococcus took place 3 days later.

Conclusion. Use of Filetol ointment contributed to preventing the generalization of infection process in mice which affected the growth of the animal survival by 25% compared to the positive control and by 12.5% compared to Levomecol ointment as well as acceleration of healing of purulent wounds.