The study of antimicrobial action of combination of ciprofloksacin with antibiotics against polyantibiotic-resistant strains of Pseudomonas aeruginosa by of checkerboard method

Abstract

Pseudomonas aeruginosa is one of the man caused of opportunistic infections.The rapid decrease in sensitivity of pathogens septic infections to antimicrobial agents has led to significant difficulties in the treatmens  of  antibioticresistant infections . One solution to this problem is a method of combining antimicrobial medications of different pharmacological groups.Antimicrobial synergy resulting from antibiotic combination therapy is often important in the treatment of serios bacterial infections.Materials and methods. Pseudomonas aeruginosa strains used in this study were isolated  from hospital-ised patients at the Hospital of the Surgery of Charkow. The study of combinations of antibiotics efficacy was carried out by determining the minimum inhibitory concentrations using routine method in vitro method "checkeboard". Fractional inhibitory concentration indices were calculated for all isolates with all combi-nations.The present  paper investigates the synergic effects of  ciprofloksacin in combination with tienam,amikacin or cefepim.Results and discussion. Calculation  of the FIX showed that the combination of ciprofloxacin with cefepim demonstrated synergistic inhibitory activity against  66,6  %   of P.aeruginosa strains tested. Thus studies have shown  the indifferent summation effects of com-binations of ciprofloxacin with tienam or with amika-cin  on the mayoriti of multiresistent strains of P.aeruginosa.Conclusion. The result of experimental study by of  checkerboard method was shown the high effective-ness of the combinations of ciprofloxacin and cefepime in relation to Pseudomonas aeruginosa poly-antibiotic-resistant strains 

Pseudomonas aeruginosa is one of the man caused of opportunistic infections.The rapid decrease in sensitivity of pathogens septic infections to antimicrobial agents has led to significant difficulties in the treatmens  of  antibioticresistant infections . One solution to this problem is a method of combining antimicrobial medications of different pharmacological groups.Antimicrobial synergy resulting from antibiotic combination therapy is often important in the treatment of serios bacterial infections.

Materials and methods. Pseudomonas aeruginosa strains used in this study were isolated  from hospitalised patients at the Hospital of the Surgery of Charkow. The study of combinations of antibiotics efficacy was carried out by determining the minimum inhibitory concentrations using routine method in vitro method "checkeboard". Fractional inhibitory concentration indices were calculated for all isolates with all combinations.The present  paper investigates the synergic effects of  ciprofloksacin in combination with tienam,amikacin or cefepim.Results and discussion. Calculation  of the FIX showed that the combination of ciprofloxacin with cefepim demonstrated synergistic inhibitory activity against  66,6  %   of P.aeruginosa strains tested. Thus studies have shown  the indifferent summation effects of combinations of ciprofloxacin with tienam or with amikacin  on the mayoriti of multiresistent strains of P.aeruginosa.Conclusion. The result of experimental study by of  checkerboard method was shown the high effectiveness of the combinations of ciprofloxacin and cefepime in relation to Pseudomonas aeruginosapolyantibiotic-resistant strains