Kary Mullis - bright name in the history of science

I Korovaeva; N Popova

Автор(и)

I Korovaeva Mechnikov Institute of Microbiology and Immunology,
N Popova Mechnikov Institute of Microbiology and Immunology,

Ключові слова:

Kary Mullis, polymerase chain reaction, DNA amplification, oligonucleotide primers.

Анотація

The article is devoted to the Karry Mullis– American biochemist, winner of Nobel Prize in Chemistry and to the history of discovery of polymerase chain reaction thanks to which rapid release of large number of specific DNA fragments becomes possible. This discovery made real revolution in molecular biology. The scientists had to work his hard way up from the discovery of the PCR to the recognition. Today, the polymerase chain reaction has been widely used in medicine, veterinary medicine, biology, criminology, history, archeology and other fields of human activity. PCR amplification of DNA is used around the world, becoming routine and daily tool in every molecular biology laboratory. This method is the most sensitive and specific method for diagnosis of infectious diseases. Thanks to PCR many modern scientific problems, genotyping of organisms, diagnosis of genetic diseases and the identification of predisposition to them are successfully solved. Application of PCR makes possible accurately to establish family ties, identify the person and made an analysis of ancient remains and identify the genetically modified organisms. And all this is giving the benefit to the fact that our contemporary Kerry Mullis joined previous achievements by his sharp analytical mind and discovered creative approach to the solving of existing scientific problems in biochemistry and molecular biology.

Посилання

Telkov, M.V. Kary Mullis, inventor of PCR [Text] / M.V. Telkov // M. Chemistry and Life. – 2006. – № 8. – P. 6-10.

Mullis, Kary B. The unusual story of how was born polymerase chain reaction [Text] / Kary B. Mullis // In the world of science. – 1990. – № 6. –

P. 1-14.

Mahony, J.B. Detection of respiratory viruses by molecular methods [Text] / J.B. Mahony // Clin. Microbiol. Rev. – 2008. – Vol.21 (4). – P.716-747.

Rybicki, E. A PCR [Text] / E. Rybicki // In Manual of Online Molecular Biology Techniques. – Univ. of Cape Town. – 2005. – 38 p.

The polymerase chain reaction in the practice of veterinary medicine [Text] / Stegnyi B.T. [et al.] // Kharkiv National Scientific Center «Institute of Experimental and Clinical Veterinary Medicine». – 108 p.