Research of antimicrobic activity of foaming products samples with octopirox

Abstract

Introduction.  Seborrheic dermatitis (SD) - chronic recurrent skin disease, which is associated with increased cutaneous fatty secretion, change in its qualitative composition and is characterized by localization in the areas of accumulation of sebaceous glands on the scalp, face, upper torso. Therefore, the choice of tactics for the treatment of patients with SD depends on the degree of clinical manifestations, duration of the disease, information about the effectiveness of previously conducted therapy. Particular attention is paid to the choice of antifungal medicines in the treatment of SD. Traditionally, local remedies in the form of ointments or creams are used to treat SD of the scalp, they cause many inconveniences when used. In this respect, foaming products, in particular shampoos, which include active substances that have certain therapeutic potential regarding the main mechanisms of SD treatment, having self-regulating, anti-inflammatory, antifungal, antibacterial, reparative, moisturizing properties, etc. are very appropriate. One of the main factors in the development of this disease is the yeastlike fungus of Malassézia fúrfur genus (or Pityrósporum ovále), which is present in the skin of each person. Octopirox®, which has strong fungicidal (antifungal) and antibacterial actions, was chosen among a number of modern products in this field. Therefore, the main purpose of our experiment was to study the antimicrobial activity of developed samples of foaming agents with octopirox to justify the rational concentration. Materials and methods. For this study, we produced a number of experimental samples of foaming bases with octopirox and α-lipoic acid at different concentrations: sample № 1 -  foaming base; sample № 2 – foaming base +  α-lipoic acid; sample № 3 – foaming base + octopirox (0,25 %); sample № 4 – foaming base + octopirox (0,5 %); sample № 5 – foaming base + octopirox (0,75 %); sample № 6 – foaming base + octopirox (1,0 %). The antimicrobial activity of prototype gels was studied in vitro by diffusion in agar (“wells” method). This method is based on the ability of active substances to diffuse in the agar medium, which was previously inoculated by bacterial crops. The results of the studies make it possible to characterize both the antimicrobial activity of the samples and the release of antimicrobial substances from the base, because the growth inhibition zones of microorganisms are formed as a result of the diffusion of these substances into a dense nutrient medium. Results. Based on the experimental data, in the development of the foaming product composition with octopirox AFI concentration of 0.5% is optimal and further increase it to 0.75% (sample No. 5) and 1.0% (sample No. 6) is impractical. It should be noted that the studies showed that foaming samples had high antifungal activity and moderate effect on gram-negative bacteria, so in the development of the optimal composition of the tool and its long-term storage and use, it is necessary to consider the introduction of preservatives and conduct appropriate research.  Conclusion. Thus, the results of the experiments showed that the test samples No. 1 and No. 2 did not have any antimicrobial action pertaining to gram-positive, gram-negative bacterial cultures and antifungal activity. Samples No. 3, No. 4, No. 5 and No. 6 of the foaming product with octopirox at concentrations of 0.25%, 0.5%, 0.75% and 1.0% have a broad spectrum of antimicrobial action pertaining to gram-positive (Staphylococcus aureus ATSC 25293 and spore cultures of Bacillus subtilis ATSS 6633), gram-negative (Escherichia coli ATSS 25922 and Pseudomonas aeruginosa ATCC 27853) bacterial cultures, as well as in relation to the cultures of Candida albicans fungi ATSS 885-653 and Aspergillus niger fungus ATCC 16404. However, the experimental results showed that sample No 4 (octopirox concentration of 0.5%) was the most promising for further work on the development of antimicrobial activity of the foaming product.