Screening of antimicrobial properties of ethanolic extracts from some kinds of raw materials with quinone derivatives

Authors

  • N Boyko National University of Pharmacy,
  • A Zaytsev National University of Pharmacy,
  • T Osolodchenko Mechnikov Institute of Microbiology and Immunology,

Keywords:

antimicrobial properties, raw material, extracts, hydroquinones, naphtoquinones, anthraquinones.

Abstract

This paper presents data on screening of antimicrobial properties of extracts from some kinds of raw materials (18 plants) with hydroquinone, naphtoquinone or anthraquinone derivatives. Some technological parameters of extracts (density and concentration of extraneous substances) have been determined. The most appropriate microbiological method of studying antimicrobial properties of extracts, diffusion method of “well”, has been applied; special mathematic method of comparison of antimicrobial properties of extracts vector analysis has been applied in order to study and to compare antimicrobial properties of extracts.  Indexes of antimicrobial properties of extracts have been determined: a complex index of medicinal product antimicrobial activity for quantitative estimation of antimicrobial effect - A, and square of correlation coefficient - r², which demonstrates the spectrum of antimicrobial activity of the extracts (degree of similarity to the standard). The most active extracts have been selected; they have antimicrobial properties of medium strength: from the herb of chimaphila umbellata А=2.20; the fruits of rhamnus cathartica А=2.12; the root of rubia tinctorum А=2.11; the bark of frangula alnus А=2.05; the root of rumex confertus А=2.04; the leaf of pyrola rotundifolia А=2.00; and leaf of arctostaphylos uva-ursi А=2.08 (but extract from uva-ursi did not affected on 2 strains of microorganisms r²=0.64). Low levels of antimicrobial activity have been demonstrated by the extract obtained from the leaf of urtica dioica А=0.72, r²=0.34. The mean result of the complex index of antimicrobial activity for the most of extracts from plants containing quinonederivatives is A = 1.77 (on 70% vol. ethanol at a ratio of raw material : extracting agent – 1:7 wt. : vol.) and may range from 0.68 to 2.85. The mean result of the correlation coefficient is r = 0.93 and may range from 0.59 to 0.99. The mean result of the concentration of extractives in extracts is C = 0.0386 g/g extract and may range from 0.0017 to 0.0755 g/g extract. The mean result of the extract density is ρ = 0.897 g/cm³ and may range from 0.877 to 0.917 g/cm³. It is noted antimicrobial properties of the solution of alizarin 0.1% m / m in 70% vol. ethanol, which in studies showed moderate strength antimicrobial properties: A = 1.60 and inhibited the growth of all tested strains of microorganisms r=0.99. This potentially allows to predict the antimicrobial properties of extracts from plants containing derivatives of alizarin on its concentration in them. Study data show significant antimicrobial properties of numerous kinds of raw materials that contain of hydroquinones, naphtoquinones, anthraquinones and high possibility of their use in complex phytochemical medicinal products as antimicrobial component.

References

Mashkovskiy M. D. Medications. - 16th ed., Rev. and ext. - M.: New Wave, 2012 - 1216 p.

Sumthong P. Activity of Quinones from Teak (Tectona grandis) on Fungal Cell Wall Stress / P. Sumthong, R. A. Damveld, Y. H. Choi, M. Arentshorst and others // Planta Med., 2006. – №72(10). – Р. 943-944.

Muzychkina R. A. Natural anthraquinones: biological properties and physico-chemical characteristics. – M.: Phasis, 1998. – 857 p.

State Pharmacopoeia of Ukraine / State Enterprise "Ukrainian Scientific Centre Pharmacopoeial quality of drugs." – 1st Ed. – Appendix 3. – Kharkov: State Enterprise "Ukrainian Scientific Centre Pharmacopoeial quality of drugs". – 2009. - 280 p.

State Pharmacopoeia of Ukraine / State Enterprise "Scientific and Expert Pharmacopoeias Centre." – 1st Ed. – H.: RIREH, 2001. - Appendix 1. – 2004. – 520 p.

The study of the specific activity of antimicrobial drugs / Y. L. Volyanskiy, S. V. Biryukova, I. S. Gritsenko et al. – Kharkov, 2004. - 38 p.

State Pharmacopoeia of USSR : Vol. 2. General methods of analysis. Medicinal herbs / USSR Ministry of health. - 11th Ed. – Moscow : Medicine, 1989. - 400 p.

Basic laboratory techniques in clinical bacteriology / Ed. WHO. - Geneva, 1994. – 131p.

Standardization of the preparation of microbial suspensions / Y. L. Volyanskiy, L. G. Mironenko, S. V. Kalinichenko and others. // Newsletter of innovations in health care № 163-2006. Ministry of Health Care of Ukraine; Ukrainian Centre for Scientific Health Information and Patent licensing work (Ukrmedpatentinform). – Kiev, 2006.

Boyko N. N. Vector algebra theory in analysis of properties of antibacterial medications / N. N. Boyko, A. I. Zaytsev, T. P. Osolodchenko // Annals of Mechnikov Institute. – 2014. - №1. – P. 20-26.

Boyko N. N. Pharmacoeconomic analysis of antibacterial medications used in dentistry / N. N. Boyko, A. I. Zaytsev, L. V. Nefedova, L. V. Yakovleva // CLINICAL PHARMACY. – 2014. – Vol. 18, No. 1. – P.59-64.

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How to Cite

Boyko, N., Zaytsev, A., & Osolodchenko, T. (2020). Screening of antimicrobial properties of ethanolic extracts from some kinds of raw materials with quinone derivatives. Annals of Mechnikov’s Institute, (4), 67–72. Retrieved from https://journals.uran.ua/ami/article/view/208225

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Research Articles