The study of antimicrobial activity of combinations of fosfomycin with cefepime and fosfomycin with tiename in respect polyantibiotic-resistant strains of Pseudomonas aeruginosa

Abstract

The rapid decrease in sensitivity of pathogens septic infections to antimicrobial agents has led to significant difficulties in the fight against antibiotic-resistant infections even in developed countries. One solution to this problem is a method of combining antimicrobial different pharmacological groups. The most promising for combination drugs are derivatives of phosphonic acids, which are able to deeply penetrate biological film and microbial cells and enhance the antibacterial action of other antibiotics. A study of the effectiveness of combination antibiotic fosfomycin with cefepime and fosfomycin with tiename on polyantibiotic-resistant strains Pseudomonas aeruginosa. Set the antibiotic used in the experiments performed by P.aeruginosa strains of "serial dilutions" and the disco-diffusion method. The efficacy of combinations of antibiotics was carried out by determining the minimum inhibitory concentrations routine method in vitro method "checkerboard". The results of experimental studies the combination of cefepime on multiresistant strains Fosfomycin on Pseudomonas aeruginosa show a significant decrease in the MIC of cefepime in combination on ten of the thirteen strains. MIC Fosfomycin significantly decreased relative to the nine strains. In calculating the index FIX appears that a synergistic effect of the combination of antibiotics studied (FIX ≤ 0,5) was observed on 69.2% of the subjects strains of  P.aeruginosa. In experiments on three strains observed effect summation antimicrobial antibiotics specified combinations (FIX> 0,5, ≤ 1,0), one strain - indifferent effect (FIX> 1.0). You can also combined fosfomycin with tiename significant (greater than 2-fold) reduction in MIC these antibiotics was observed only for 2 subjects cultures P. aeruginosa. Calculation of the FIX showed that combined use of fosfomycin with tiename created largely indifferent effect refers to the strains of P. aeruginosa. Thus, studies have shown a high degree of synergy combination of fosfomycin with cefepime on polyantibiotic-resistant strains strains of P. aeruginosa. The rapid decrease in sensitivity of pathogens septic infections to antimicrobial agents has led to significant difficulties in the fight against antibiotic-resistant infections even in developed countries. One solution to this problem is a method of combining antimicrobial different pharmacological groups. The most promising for combination drugs are derivatives of phosphonic acids, which are able to deeply penetrate biological film and microbial cells and enhance the antibacterial action of other antibiotics.A study of the effectiveness of combination antibiotic fosfomycin with cefepime and fosfomycin with tiename on polyantibiotic-resistant strains Pseudomonas aeruginosa. Set the antibiotic used in the experiments performed by P.aeruginosa strains of "serial dilutions" and the disco-diffusion method. The efficacy of combinations of antibiotics was carried out by determining the minimum inhibitory concentrations routine method in vitro method "checkerboard".The results of experimental studies the combination of cefepime on multiresistant strains Fosfomycin on Pseudomonas aeruginosa show a significant decrease in the MIC of cefepime in combination on ten of the thirteen strains. MIC Fosfomycin significantly decreased relative to the nine strains. In calculating the index FIX appears that a synergistic effect of the combination of antibiotics studied (FIX ≤ 0,5) was observed on 69.2% of the subjects strains of P.aeruginosa. In experiments on three strains observed effect summation antimicrobial antibiotics specified combinations (FIX> 0,5, ≤ 1,0), one strain - indifferent effect (FIX> 1.0).You can also combined fosfomycin with tiename significant (greater than 2-fold) reduction in MIC these antibiotics was observed only for 2 subjects cultures P. aeruginosa. Calculation of the FIX showed that combined use of fosfomycin with tiename created largely indifferent effect refers to the strains of P. aeruginosa.Thus, studies have shown a high degree of synergy combination of fosfomycin with cefepime on polyantibiotic-resistant strains strains of P. aeruginosa.