The effect of collagen-chitosan-natrium hyaluronate composite on neovascularization as angiogenesis reaction in rabbit corneal stroma wound (Experimental Study on Oryctolagus cuniculus)

Abstract

Background: The inflammatory process in the healing of corneal wounds can lead to cicatricial tissue resulting in cloudiness, and blood vessels in the corneal tissue cause visual disturbances to blindness. Keratoplasty is the primary therapeutic modality to replace pathological corneal tissue. Due to the limited availability of donors, many alternative therapies have been developed to restore corneal regeneration, one of which is the use of biomaterials in prosthetic devices with composite materials. Collagen-chitosan-sodium hyaluronic composite is a natural biopolymer that is considered to have good biocompatibility, biodegradable, and non-toxic properties so that it is hoped that we can obtain advantages. Besides being easy to get and produce, the composite is safe and can increase corneal tissue regeneration.  Methods: Twenty adults New Zealand male white rabbits (30 eyes) were divided into three groups. The first group is control negative, the left eye without any treatment. The second group is control positive gro, which was the right eye with the corneal stromal injury. The third group was the right eye with corneal stromal injury and implantation of the collagen-chitosan-sodium hyaluronic composite. On day 14, the sedation was performed, and the degree of neovascularization was assessed clinically. Results: The study results 14 days after treatment showed a significant difference in the level of neovascularization in the stromal injury group with the collagen-chitosan-sodium hyaluronic composite implantation group (p = 0.012, α> 0.05). The type of neovascularization indicating that the wound healing process is ongoing. Conclusion: There was a significant difference in the level of neovascularization between the stromal injury group and the collagen-chitosan-sodium hyaluronic composite implantation group. Keywords: cornea, biomaterials, composites, collagen, chitosan, sodium hyaluronic, neovascular.