Selection of antimicrobial substances in the rectal cream composition containing thick extract of carrot and rutin

Abstract

Introduction. Synthetic preservatives can significantly extend the shelf life of a perishable product, although they have many side effects that are harmful to human health. In view of this, there is a current trend towards the use of preservatives made from natural plant products. According to the literature, natural substances are widely used as effective preservatives. Regulatory authorities that control the quality, safety and efficacy of medicines pay great attention to natural substances that can be used in the pharmaceutical industry, which will reduce the risk of side effects.

A rectal cream with a thick extract of sowing carrot and rutin is being developed at the Department of Industrial Technology of Drugs, National University of Pharmacy, Kharkiv. It is known that the properties of flavonols and their glycosides are actively studied for their antimicrobial activity. Rutin has been found to inhibit the growth of Escherichia coli, inhibit the vital activity of Proteus vulgaris, Shigella sonnei and Klebsiella sp., and has antimicrobial activity against Pseudomonas auruginosssa and Bacillus subtilis. Due to inhibition of DNA isomerase IV, it inhibits the activity of E. coli. Rutin synergistically enhances the antibacterial activity of other flavonoids against Bacillus cereus and Salmonella enteritidis, and has antifungal activity against Candida gattii strain with a minimum inhibitory concentration of 60 μg/ml. Samples of rectal cream with rutin and thick carrot extract without and with the addition of various antimicrobial agents were tested. Material & methods. The objects of the study were : Microcare PE (Phenoxyethanol) 1%, Sharomix 702 (Dehydroacetic Acid, Benzoic Acid, Phenoxyethanol) ( 1.35%), Sharomix MCI II (Methylchloroisothiazolinone, Methylisothiazolinone and Benzyl Alcohol) 0.1%, Sharomix 300 (Me, Pr-paraben, Bronopol, Phenoxyethanol) 0.7%, Sharomix Amplify AM-25 (Phenoxyethanol, Chlorphenesin, Caprylyl Glycol, Didecyldimonium chloride) 0.5%, Sharomix EG10 (EthylHexylGlycerin, Phenoxyethanol) 1.1%, SharoSENSE Plus 181 (Maltol, Polyquaternium-80) 0.5%. A sample of rectal cream with and without added preservatives was studied. Results & discussion. The minimum inhibitory concentrations of the studied preservatives for E. coli culture were determined using the method of serial dilutions. The efficacy of antimicrobial preservatives was tested, the growth properties of culture media were checked, and the suitability of the method for determining the total number of viable cells was verified. The efficacy of SharoSENSE Plus 181 and Sharomix Amplify AM-25 in the composition of rectal cream was investigated. The compliance of the obtained results with the requirements of the SFU for drugs for rectal use was established. Conclusions. It was found that the most effective were SharoSENSE Plus 181 and Sharomix Amplify AM-25, which showed an inhibitory effect at a concentration of 480 ppm and 500 ppm, respectively. It was found that the culture media were suitable for growth properties in accordance with the requirements of SFU 2.6 (2.6.12), and the test microorganisms corresponded to the taxonomic characteristics - the morphology of colonies on culture media and cell morphology during microscopy were typical for the corresponding strain. Taking into account the requirements of safety, economy and physicochemical properties, SharoSENSE Plus 181 at a concentration of 0.5% was chosen as the most acceptable preservative.

Keywords: antimicrobial substances, rectal cream, composition, thick extract of carrot, rutin, minimum inhibitory concentrations, effectiveness of antimicrobial preservatives