Microalgae cultivation using wastes

Authors

  • Наталія Борисівна Голуб National technical university of Ukraine “Kyiv Polytechnic Institute” 4 Housing, 37 Peremogy Ave, Kyiv, 03056, Ukraine, Ukraine

DOI:

https://doi.org/10.15587/1729-4061.2013.19121

Keywords:

Chlorella vulgaris, manure, cultivation, waste, photoreactor, lipids, biomass, microalgae

Abstract

The process of cultivation of Chlorella vulgaris in photoheterotrophic conditions at illumination  12 hours of light - 12 hours of darkness is studied. It is shown that the sugar beet extract in concentrations of 0.0 - 0.05 mol/L (on glucose), added to the nutrient medium Gromov №6 positively influences the gain of the biomass of microalgae Chlorella vulgaris. The greatest gain of microalgae biomass (35∙106 cells/mL) occurs at simultaneous use of mineral (CO2) and organic (extract) carbon. It is shown that the gain of biomass and lipids depends on the nutrition source on nitrogen. The yield of biomass and lipids increases in conditions of nitrogen deficiency in the culture medium at replacing the nitrate form of nitrogen with the ammonium form. Using the extract from poultry manure, the concentration of lipids reaches 26% (in medium Gromov №6 - 21%, modified on ammonium - 24 %). Optimum concentration of the total nitrogen is up to 0.035 mol/L. The increase in the biomass gain using the extract from the manure is explained by the presence in the medium of nitrogen, primarily in the nitrate and ammonium forms and urea, as well as organic substances (amino acids, carbohydrates, etc.), which are used without the decomposition of Chlorella vulgaris and do not require additional energy sources.

Author Biography

Наталія Борисівна Голуб, National technical university of Ukraine “Kyiv Polytechnic Institute” 4 Housing, 37 Peremogy Ave, Kyiv, 03056, Ukraine

Associate Professor Ph.D.  

Department of environmental biotechnology and bioenergy

References

  1. Chisti, Y. Biodiesel from microalgae [Текст] / Y. Chisti // Biotechnology Advances.– 2007. - № 25. – Р. 294–306.
  2. Becker, E. W. Microalgae: biotechnology and microbiology [Текст] / E. W. Becker .- Cambridge University Press, 1994. – 301 p.
  3. Sheehan, J. A look back at the U.S. Department of Energy’s aquatic species program – Biodiesel from algae [Текст] / Sheehan John. – Colorado: The National Renewable Energy Laboratory, 1998. – 296 p.
  4. Голуб, Н. Б. Водорості як сировина для одержання біодизельного пального [Текст] / Н. Б. Голуб, В. Ю. Бунча // Відновлювальна енергетика. - 2010. - № 2. - С. 79-86.
  5. Martinez, F. Interactions between Glucose and Inorganic Carbon Metabolism in Chlorella vulgaris Strain UAM 1011 [Текст] / Flor Martinez, Maria Isabel Orus // Plant Physiol. – 1991. - №95. - P. 1150-1155.
  6. Xu, H. High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters [Текст] / Han Xu, Xiaoling Miao, Qingyu Wu // Journal of Biotechnology. – 2006. - № 126. – P. 499–507.
  7. Shi, X.-M. Production of biomass and lutein by Chlorella protothecoides at various glucose concentrations in heterotrophic cultures [Текст] / Xian-Ming Shi, Hui-Jun Liu, Xue-Wu Zhang, Feng Chen // Process Biochemistry. – 1999. - V. 34.- N 4. - P. 341–347.
  8. Heredia-Arroyo, Т. Mixotrophic cultivation of Chlorella vulgaris and its potential application for the oil accumulation from non-sugar materials [Текст] / Tamarys Heredia-Arroyo, Wei Wei, Roger Ruan, Bo Hu // Biomass and Bioenergy. – 2011. - V. 35. N 5. – P. 2245–2253.
  9. Valderramaa, Luz T. Treatment of recalcitrant wastewater from ethanol and citric acid production using the microalga Chlorella vulgaris and the macrophyte Lemnaminuscula [Текст] / Luz T. Valderramaa, Claudia M. Del Campoa, Claudia M. Rodrigueza, Luz E. de- Bashana, Yoav Bashan // Water Research. – 2002. - № 36. – Р.4185–4192.
  10. Penglin, Li. In Situ Biodiesel Production from Fast-Growing and High Oil Content Chlorella pyrenoidosa in Rice Straw Hydrolysate [Текст] / Li Penglin, Miao Xiaoling, Li Rongxiu, Zhong Jianjiang // Journal of Biomedicine and Biotechnology. – 2011, V. Article ID 141207, 8 p.
  11. Komor, Е. The Hexose-Proton Symport System of Chlorella vulgaris. Specificity Stoichiometry Energetics of Sugar-Induced Proton Uptake [Текст] / Ewald Komor, Widmar Tanner // European Journal of Biochemistry. – 2005. - №44. - Р. 219–223.
  12. Hu, Q. Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances [Текст] / Q. Hu, M. Sommerfeld, E. Jarvis, M. Ghirardi, M. Posewitz, M. Seibert, A. Darzins // The Plant Journal. – 2008. – №54. – Р. 621–639.
  13. Basova, M. M. Fatty acid composition of lipids in microalgae [Текст] / M. M. Basova // International Journal on Algae. – 2005. – №7. – Р. 33–57.
  14. Муззафаров, А. М. Культивирование и применение микроводорослей [Текст] / А. М. Муззафаров, Т. Т. Таубаев .- Ташкент: Фан, 1984. - 185 с.
  15. Gribovskaia, I. V. Usage of urina in food Chlorella vulgaris [Текст] / I. V. Gribovskaia, G. S. Kalacheva, L. S. Tirranen, A. A. Kolmakova, Yu. I. Baianova // J. Siberian federal University. Biologe. - 2011. - N3. - P. 243-256.
  16. Xin, Li Growth and nutrient removal properties of a freshwater microalga Scenedesmus sp. LX1 under different kinds of nitrogen sources [Текст] / Li Xin, Hu Hong-ying, Gan Ke, Yang Jia // Ecological Engineering. - 2010. – V.36. - N4. - P. 379-381.
  17. Упитис, В. В. Макро и микроэлементы в оптимизации минерального питания микроводорослей [Текст] / В. В. Упитис. - Рига: «Знание», 1983. - 239 с.
  18. Харитонов, Ю. Я. Аналитическая химия (аналитика): В 2 кн. Кн. 2. Количественный анализ. Физико-химические (инструментальные) методы анализ: учебник [Текст] / Ю. Я Харитонов. – М.: 2001. – 459 с.
  19. Великая, Е. И. Лабораторный практикум по курсу общей технологии бродильных производств (общие методы контроля): учебное пособие [Текст] / Е. И. Великая. – М.: Легкая и пищевая пром-сть, 1983. – 312 с.
  20. Bligh, E. G. A rapid method for total lipid extraction and purification [Текст] / E. G. Bligh, W. J. Dyer // Can. J. Biochem.Physiol. – 1959. – №37. – P. 911-917.
  21. Голуб, Н. Б., Бунча В. Ю. Вплив іонів лужних металів на приріст біомаси та накопичення ліпідів у Chlorella vulgaris [Текст] / Н. Б. Голуб, В. Ю. Бунча // Наукові вісті НТУУ «КПІ». - 2012. - №3. - С.12-17.
  22. Chisti, Y. (2007). Biodiesel from microalgae. Biotechnology Advances, 25, 294–306.
  23. Becker, E. W. (1994). Microalgae: biotechnology and microbiology. Cambridge University Press, 301.
  24. Sheehan, J. (1998). A look back at the U.S. Department of Energy’s aquatic species program – Biodiesel from algae. The National Renewable Energy Laboratory, Colorado, 296.
  25. Golub, N. B., Buncha, V. Yu. (2010). Algae as a feedstock for biodiesel production. Renewable energy, 2, 79-86.
  26. Martinez, F., Orus, V. I. (1991). Interactions between Glucose and Inorganic Carbon Metabolism in Chlorella vulgaris Strain UAM 1011. Plant Physiol., 95,1150-1155.
  27. Xu, H., Miao, X., Wu, Q. (2006). High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters - Journal of Biotechnology, 126, 499–507.
  28. Shi, X. M., Liu, H. J., Zhang, X. W., Chen, F. (1999). Production of biomass and lutein by Chlorella protothecoides at various glucose concentrations in heterotrophic cultures. Process Biochemistry, 34 (4), 341–347.
  29. Heredia-Arroyo, T., Wei, W., Ruan, R., Hu, B. (2011). Mixotrophic cultivation of Chlorella vulgaris and its potential application for the oil accumulation from non-sugar materials. Biomass and Bioenergy, 35 (5), 2245–2253.
  30. Valderramaa, L. T., Del Campoa, C. M., Rodrigueza, C. M., de-Bashana, L. E., Bashan, Y. (2002). Treatment of recalcitrant wastewater from ethanol and citric acid production using the microalga Chlorella vulgaris and the macrophyte Lemnaminuscula. Water Research, 36, 4185–4192.
  31. Li, P., Miao, X., Li, R., Zhong, J. (2001). In Situ Biodiesel Production from Fast-Growing and High Oil Content Chlorella pyrenoidosa in Rice Straw Hydrolysate. Journal of Biomedicine and Biotechnology, Article ID 141207, 8
  32. Komor, E., Tanner, T. (2005). The Hexose-Proton Symport System of Chlorella vulgaris. Specificity Stoichiometry Energetics of Sugar-Induced Proton Uptake. European Journal of Biochemistry, 44, 219–223
  33. Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., Darzins, A. (2008). Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. The Plant Journal, 54, 621–639.
  34. Basova, M. M. (2005). Fatty acid composition of lipids in microalgae. International Journal on Algae, 7, 33–57.
  35. Muzzafarov, А. M., Taubaev, T. T. (1984). Cultivation and usage of microalgae. Tashkent: Fan, 185.
  36. Gribovskaia, I. V., Kalacheva, G. S., Tirranen, L. S., Kolmakova, A. A., Baianova, Yu. I. (2011). Usage of urina in food Chlorella vulgaris. J. Siberian federal University. Biologe, 3, 243-256
  37. Xin, L., Hong-ying, H., Ke, G., Jia, Y. (2010). Growth and nutrient removal properties of a freshwater microalga Scenedesmus sp. LX1 under different kinds of nitrogen sources. Ecological Engineering, 36 (4), 379-381.
  38. Upitis, V. V. (1983). Macro and microelements in the optimization of mineral nutrition of microalgae, 239.
  39. Haritonov, Yu. Ya. (2001). Analytical Chemistry (analytics) in 2 books. Book. 2. Quantitative analysis. Physico-chemical (instrumental) methods of analysisl, 459.
  40. Velikaya, E. I. (1983). Laboratory Practicum on general zymurgy (general methods of control). M.: Consumer industry and food processing, 312.
  41. Bligh, E. G., Dyer, W. J. (1959). A rapid method for total lipid extraction and purification. Can. J. Biochem.Physiol,37, 911-917
  42. Golub, N. B., Buncha, V. Yu. (2012). Effect of alkali metal ions on the growth of biomass and lipid accumulation in Chlorella vulgaris. Scientific Proceedings of NTUU "KPI", 3, 12-17

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Published

2013-12-11

How to Cite

Голуб, Н. Б. (2013). Microalgae cultivation using wastes. Eastern-European Journal of Enterprise Technologies, 6(10(66), 4–9. https://doi.org/10.15587/1729-4061.2013.19121

Issue

Vol. 6 No. 10(66) (2013): Ecology. Materials science

Section

Ecology

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Copyright (c) 2014 Наталія Борисівна Голуб

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