The dominance degree of tocopherol contents in F1 sunflower hybrids
DOI:
https://doi.org/10.30835/2413-7510.2017.120439Keywords:
sunflower, sunflower oil, tocopherol, antioxidantAbstract
The aim and tasks of the study.The primary objective of this work was to investigate male forms and sunflower hybrids of the first generation for the contents of different tocopherol isomers. Segregation of the first generation hybrids was also investigated, and lines with increased contents of β, γ and δ-isomers of tocopherols were selected.
Material and methods.The studies were carried out in lines with high contents tocopherol isomers and in lines developed in the Laboratory of Sunflower Breeding and Genetics of the Plant Production Institute named after VYaYuriev of NAAS.
The tocopherol content and composition were analyzed by high-performance liquid chromatography.
Results and discussion.The article presents the results of studying the contents of tocopherol isomers in sunflower lines bred at the PPI as well as in lines developed in collaboration with the All-Russian Research Institute of Oil Crops. It was established that α-tocopherol predominated in sunflower lines bred at the PPI. Its content varied from 4.12 mg% (Kh279V) to 12.36 mg% (Kh220V). Lines developed in collaboration with the All-Russian Research Institute of Oil Crops containd a contrasting pattern of tocopherol isomers, which allows using them in sunflower breeding to obtain accessions with various contents of tocopherol isomers. The content of α-tocopherol ranges from 3.73 mg% (Vk-L-2) to 10.14 mg% (Vk-L-5); of β-tocopherol - from 1.08 mg% (Vk-L-2) to 22,52 mg% (Vk-L-4); of γ-tocopherol - from 0.54 mg% (Vk-L 1) to 6.60 mg% (Vk-L-4); and of δ-tocopherol - from 0.53 mg % (Vk-L-4) to 21.80 mg% (Vk-L-1).
Peculiarities of the inheritance of different isomers of tocopherols in the first generation hybrids were also studied. Tests showed that the content of α-tocopherol inherited by overdominance in all hybrid combinations.
Conclusions.It was found that various contents of tocopherols in the first-generation hybrids depended on male components. Choosing male components, one can obtain the first generation hybrids with different composition of tocopherol isomersReferences
KyrychenkoVV. Sunflowerbreedingandseedproduction(HelianthusannusL.).Kharkiv, 2005. 385 p.
VlesRO, Gottenbos JJ. Nutritional characteristics and food uses of vegetable oil.Cambrige: Mc Grow-Hill Publ. Corp., 1989. P. 63–86.
Beare-RogersJ. Food fatsandfattyacidinhumannutrition.Eds. Champaigue, AOCS, 1995. P. 1–7.
Zinchenko OI, Salatenko VN, Bilonozhko MA.PlantProduction.Кyiv: Agrarnaosvita, 2001. 591 p.
NadirovNK. Tocopherols and their use in medicine and agriculture. 1991. 336 p.
ReedLG, HarriRS, WoolLG, MarrionGF, ThimannKV. Biochemistryoflipoicacid.Vitaminshormons.Acaolpress.1962; 20: 1–38.
McHale D, Mamalis P, Green J, Marcinkiewicz S. Tocopherols. 1. Synthesis of 5-metyltocol. Ibid. 1959; 11: 3362–3368.
McHaleD,GreenJ, FeeneyC, SutcliffeF. Tocopherols. 8. Structuralandsyntheticstudiesofα-tocopherol.Ibid. 1963; 2: 784–786.
DemurinYaN. Genetic analysis of the tocopherol composition in sunflower seeds.Nauchno-tekhnicheskiybulleten VIR.1986; 165: 49–51.
DemurinYaN. Geneticvariabilityoftocopherolcompositioninsunflowerseeds.Helia. 1993; 16(18): 59–62.
Genetic studies of sunflower oil quality traits in the All-Russian Research Institute of Oil Crops: main results of research on oil crops (to the 100th anniversary of the All-Russian Research Institute of Oil Crops). Krasnodar. The State Scientific Institution “All-Russian Research Institute of Oil Crops named after VS Pustovoyt” of the Russian Academy of Agricultural Sciences.2012.
Velasco L, Fernandez-Martinez JM. Identification and genetic characterization of new sources of beta and gamma tocopherol in sunflower germplasm.Helia. 2003; 26(38): 17–24.
Velasco L, Peres-Vich B, Fernandez-Martinez JM. Development of sunflower germplasm with high delta tocopherol content. Helia. 2004; 27(40): 99–106.
Velasco L, Fernandez-Martinez JM. Registration of T589 and T2100 sunflower germplasms with modified tocopherol profiles. Crop Science.2004; 44: 361–362.
Velasco L, Peres-Vich B, Fernandez-Martinez JM. Novel variation for tocopherol profile in sunflower created by mutagenesis and recombination.Plant Breed.2004; 123: 490–492.
Velasco L, Peres-Vich B, Fernandez-Martinez JM, Dominguez-Gimenez J. Novel variation for tocopherolprofilein sunflower. In.: Proc 16th Intl. Sunflower Conf. 2004; 2: 793–798.
Velasco L,Peres-Vich B, Fernandez-Martinez JM. Evaluation of wild sunflower species for tocopherol content and composition. Helia. 2004; 27(40): 107–112.
DemurinYaN,Borisenko OM, Peretyagina TM, Perstenyeva AA.Gene linkage test for imr with Ol, tph1and tph2 mutations in sunflower.Helia.2005; 29(44): 41–46.
Peres-Vich B, Berry ST, Velasco L et al. Molecular mapping of nuclear mole sterility genes in sunflower. Crop.Sci. 2005; 54: 1851–1857.
Del Moral L, Fernandez-Martinez JM, Peres-Vich B, Velasco L. Expression of modified tocopherol content and profile in sunflower tissue. J. Sci. Food. Agric. 2012; 92: 351–357.
Berry ST, Leon AJ, Hanfrey CC et al. Molecular marker analysis of Helianthus annus L. 2.Construction of an RFLP linkage map for cultivated sunflower.Theor. Appl. Genet. 1995; 91: 195–199.
ŠkoricD, JocicS, JovanovicDetal. Achievementsofsunflowerbreeding.Zbornicradova-IFVC, NS.Book 42.131–137.
State standard of Ukraine EN 2822:2005. Determination of vitamin E content by high-resolution liquid chromatography of α-, β-, γ- іδ- tocopherols: valid from 01. 07.2006. Кyiv: DerzhspozhyvstandartUkrainy, 2006. 13 p.
DospekhovBA. Methods of field experience. Moscow: Agropromizdat, 1985. 351 p.
Downloads
Published
Issue
Section
License
Copyright (c) 2018 Н. С. Харитоненко, В. В. Кириченко
This work is licensed under a Creative Commons Attribution 4.0 International License.
When placing the text in electronic resources, the copyright is reserved by the author of a publication.
Authors may not agree with referee’s(s’) comments and remarks of the Editorial Board, rationalizing their point of view.
Authors may require clarification from or the Editorial Board or changes in the event of significant errors in their article.
Authors can use materials published in the journal Selection and Seed Industry in their work, mandatorily referring to our journal.
