Effects of different storage regimens on the rye seed germination and abscisic acid content
DOI:
https://doi.org/10.30835/2413-7510.2020.206978Keywords:
rye seeds, storage, water content, longevity, abscisic acidAbstract
Rye (Secale cereale L.) seed often causes certain difficulties during long-term storage. Earlier common recommendations for modes of rye seed storage were specified.
Purpose and objective. Seed longevity of rye accessions with 5–7% seed moisture content during storage at unregulated temperature under eastern Forest-Steppe of Ukraine conditions, 4 °C and –20 °C and in the experiment modeling "accelerated aging" conditions was studied.
Material and methods. “Accelerated aging" was carried out by keeping of Kharkivianka variety seeds with a moisture content of 5%, 6% and 7% for 12, 18 months under these model conditions at 37 °C. For the studied seed samples were evaluated laboratory germination, seedling length and abscisic acid content in the seeds for individual variants of the experiment.
Results and discussion. Analysis of rye seed germination monitoring after 12, 24 and 36 months of storage shows the importance of seed storage at moisture content not exceeding 7%, achieved under appropriate drying regimes. Moisture content 5–6% is preferable for seed storage at unregulated temperature conditions of the eastern forest-steppe of Ukraine. The advantages of low storage temperatures over unregulated ones haven’t been established at these levels of moisture content after three years of storage. The best physiological condition of rye seed seedlings after two years of storage was observed at 5% seed moisture and -20 °C storage temperature.
Conclusions. According to the results of the used mode of accelerated aging it is established that it is better to predict seed longevity at a moisture content of 7%. Analysis of ABA content in seeds stored at unregulated temperature in the eastern forest-steppe of Ukraine, 4 °C and -20 °C shows that the lower ABA seed content was at lower storage temperature, but the ABA content didn’t predict seed longevity.
References
Khoroshailov NG, Zhukova NV. The experience of long-term seed storage. Trudy po prikladnoi botanike, genetike i selektsii. 1973. 49(3): 269–79.
DSTU-4522:2006. Rye. Specifications: [Internet] [Available from]: URL: http://af.gov.ua/images/stories/files/dsty4522-2006.pdf [date cited 10.10.19].
Genebank Standards for Plant Genetic Resources for Food and Agriculture. Commissionon Genetic Resources for Food and Agriculture. Rome. 2014. [Internet] [Available from]: URL: http://www.fao.org/3/a-i3704e.pdf [date cited 12.11.19].
Barzali M, Lohwasser U, Niedzielski M, Börner A. Effects of different temperatures and atmospheres on seed and seedling traits in a long-term storage experiment on rye (Secale cereale L.). Seed Science and Technology. 2005; 33(3): 713–21. DOI: 10.15258/sst.2005.33.3.19.
Hong TD, Ellis RH. A protocol to determine seed storage behavior. IPGR Technical bulletin NO.1. The University of Reading; 1996. 64 p.
Roberts EH, Ellis RH. Water and seed survival. Annals of Botany. 1989; 63: 39–52.
Zadorozhna OA, Herasimov MV, Shyianova TP. Features of long term seeds storage of rye genepool accessions. Genetičnì resursi roslin. 2014; 14: 105–14.
Whitehouse K, Hay F, Ellis R. Improvement in rice seed storage longevity from high temperature drying is a consistent positive function of harvest moisture content above a critical value. Seed Science Research. 2018; 28(32): 332–39. DOI:10.1017/S0960258518000211.
Alekseichuk GN. The vigour of cereals seed growth and its evaluation by the method of accelerated aging. Minsk: Pravo i ekonomika. 2009. 44 p.
Walters C, Engels J, Walters C, Engels J. The effects of storing seeds under extremely dry conditions. URL: https://www.bioversityinternational.org/ fileadmin/bioversity/ publications/Web_version/243/ch02.htm [date cited 10.11.18].
Zadorozhna OA, Shyianova TP, Gerasimov MV. Influence of different storage conditions оn rye (Secale cereale L.) Seed germination and antioxidant activity. Genetičnì resursi roslin. 2016; 18: 104–18.
Zadorozhna O. Some supplemental recommendations for the optimum moisture content of wheat seed for long-term storage. Annual Wheat Newsletter. Kansas State University. 2001; 47: 203–204.
Rodríguez-Gacio MC, Matilla-Vázquez MA, Matilla1 AJ. Seed dormancy and ABA signaling. Plant Signaling & Behavior. 2009; 4(11): 1035–1048. DOI: 10.4161/psb.4.11.9902.
Nambara E, Okamoto M, Tatematsu K, Yano R, Seo M, Kamiya Y. Abscisic acid and the control of seed dormancy and germination. Seed Science Research. 2010; 20: 55–67.
Abts W, Van De Poel B. Vandenbussche B., Proft, DeProfit M. Abscisic acid inhibits germination and indirectly delays ethylene biosynthesis of Beta vulgaris. Seed Science and Technology. 2015; 43 (2): 156–167. DOI: 10.15258/sst.2015.43.2.18.
Wang LL, Chen XY, Yang Y, Wang Z, Xiong F. Effects of exogenous gibberellic acid and abscisic acid on germination, amylases, and endosperm structure of germinating wheat seeds. Seed Science and Technology. 2016; 44(1): 64–76.
Mao Z, Sun W. Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of abscisic acid insensitive. Journal of Experimental Botany. 2015; 66(15): 4781–4794. DOI: 10.1093/jxb/erv244.
Rodriguez M, Bodrone M, Castellari M, Batlla D. Effect of storage temperature on dormancy release of sunflower (Helianthus annuus) achenes. Seed Science Research. 2018; 28(2): 101–111. DOI: 10.1017/S0960258518000065.
Methods of state testing of plant varieties for suitability for distribution in Ukraine. Protection of plant variety rights. Kyiv: Alefa; 2003. P. 191–203.
International rules for seed testing. 1984. Мoscow: Kolos; 311 p.
Likhachev BS, Musorina LI. Biulleten VIR. 1978; 77: 57–62.
Nikolaeva MG Obrucheva NV.,editors. Physiology and biochemistry of seed dormancy and germination 1982. Moscow:Kolos; 1982. 495 p.
Leida C, Conejero A, Arbona V, Gómez-Cadenas A, Llácer G. Chilling-dependent release of seed and bud dormancy in peach associates to common changes in gene expression. URL: http://www.ncbi.nlm.nih.gov /pubmed/22590512 [date cited 20.10.19].
Savinskii SV, Dragovoz IV, Pedchenko VK. Determination of the content of zeatin, indole-3-acetic and abscisic acids in one plant sample by high-sensitivity liquid chromatography. Fiziolohiia i biokhimiia kulturnykh rastenii. 1991; 23(6): 611–618.
Volf VG. Statistical processing of experimental data. Мoscow: Kolos, 1966. 255 p.
Downloads
Published
Issue
Section
License
Copyright (c) 2020 O. A. Zadorozhna, D. K. Yehorov, V. V. Zhmurko
This work is licensed under a Creative Commons Attribution 4.0 International License.
When placing the text in electronic resources, the copyright is reserved by the author of a publication.
Authors may not agree with referee’s(s’) comments and remarks of the Editorial Board, rationalizing their point of view.
Authors may require clarification from or the Editorial Board or changes in the event of significant errors in their article.
Authors can use materials published in the journal Selection and Seed Industry in their work, mandatorily referring to our journal.
