Investigation of food supplements with preservative e211 (sodium benzoate) using thin-layer chromatography

Authors

  • Juste Gedziute Lithuanian University of Health Sciences Sukilėlių str., 13, Kaunas, Lithuania, 50162, Lithuania
  • Mindaugas Marksa Lithuanian University of Health Sciences Sukilėlių str., 13, Kaunas, Lithuania, 50162, Lithuania
  • Augusta Zevzikoviene Lithuanian University of Health Sciences Sukilėlių str., 13, Kaunas, Lithuania, 50162, Lithuania
  • Vaidas Skyrius Lithuanian University of Health Sciences Sukilėlių str., 13, Kaunas, Lithuania, 50162, Ukraine
  • Andrejus Zevzikovas Lithuanian University of Health Sciences Sukilėlių str., 13, Kaunas, Lithuania, 50162, Lithuania

DOI:

https://doi.org/10.15587/2519-4852.2018.135758

Keywords:

thin-layer chromatography, food supplements, sodium benzoate, qualitative and quantitative determination

Abstract

Sodium benzoate is often chosen for the manufacture of liquid food supplements as preservative due to its antimicrobial effect against a large number of microorganisms. Permissible sodium benzoate amount in liquid food supplements is up to 2000 mg/l.

Methods. For investigation six liquid food supplements, containing sodium benzoate were randomly chosen. Analysis was performed using CAMAG Twin Chamber, TLC Silica Gel 60 F254 glass plates, solvent system (chloroform – ethanol 9:1) and the UV-light (254 nm) for visualization.

Results. Methods for visualisation and solvent system were selected using the reference solutions. Sodium benzoate was identificated by Rf = 0.76, it’s quantity was determined by calibration. Validated methodics was adapted for identification of sodium benzoate in liquid food supplements. The amount of sodium benzoate in all investigated objects was analysed and did not exceed the permissible amount.

Conclusions. Selected methodics are suitable for qualitative and quantitative evaluation of sodium benzoate in liquid food supplements

Author Biographies

Juste Gedziute, Lithuanian University of Health Sciences Sukilėlių str., 13, Kaunas, Lithuania, 50162

Postgraduate Student

Department of Analytical and Toxicological Chemistry

Mindaugas Marksa, Lithuanian University of Health Sciences Sukilėlių str., 13, Kaunas, Lithuania, 50162

Lecturer

Department of Analytical and Toxicological Chemistry

Augusta Zevzikoviene, Lithuanian University of Health Sciences Sukilėlių str., 13, Kaunas, Lithuania, 50162

PhD, Associate Professor

Department of Analytical and Toxicological Chemistry

Vaidas Skyrius, Lithuanian University of Health Sciences Sukilėlių str., 13, Kaunas, Lithuania, 50162

PhD, Associate Professor

Department of drug technology and social pharmacy

Andrejus Zevzikovas, Lithuanian University of Health Sciences Sukilėlių str., 13, Kaunas, Lithuania, 50162

PhD, Associate Professor

Department of Analytical and Toxicological Chemistry

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Published

2018-06-28

How to Cite

Gedziute, J., Marksa, M., Zevzikoviene, A., Skyrius, V., & Zevzikovas, A. (2018). Investigation of food supplements with preservative e211 (sodium benzoate) using thin-layer chromatography. ScienceRise: Pharmaceutical Science, (3 (13), 4–7. https://doi.org/10.15587/2519-4852.2018.135758

Issue

No. 3 (13) (2018)

Section

Pharmaceutical Science

License

Copyright (c) 2018 Juste Gedziute, Mindaugas Marksa, Augusta Zevzikoviene, Vaidas Skyrius, Andrejus Zevzikovas

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