Intensification of the extraction process of phenolic compounds from Acorus calamus leaves

Authors

DOI:

https://doi.org/10.15587/2519-4852.2021.238329

Keywords:

Acorus calamus leaves, phenolic compounds, extraction process, re-maceration, ultrasonic action, surfactant-mediated extraction

Abstract

The aim. To determine the intensified method of extraction of phenolic compounds from Acorus calamus leaves and optimal conditions for the process.

Materials and methods. In order to develop the optimal intensified method of extraction samples were prepared in different conditions of raw materials-extractant ratio, temperature, time and multiplicity. As a raw materials spectrophotometrically pre-standardized Acorus calamus leaves were used. The extraction was carried out in a hermetically sealed ultrasonic extraction reactor PEX 1 (REUS, Contes, France). As the criteria of extraction efficiency were indicators of dry residue and total amount of flavonoids determined using methods described in State Pharmacopoeia of Ukraine. The amount of flavonoids was determined spectrophotometrically on a certified device Specord 200 (Analytik Jena, Germany).

Results. According to our research results it was found that ultrasonic action and addition of surfactant significantly improves the efficiency of the extraction process. The optimal conditions for the process were determined. Experimentally proved that the rational raw material-extractant ratio is 1:15. Comparative study of the extraction process with different temperatures showed that the highest amount of extractives is achieved at temperature 70 °C and 45 min of duration. The optimal extraction multiplicity is 3.

Conclusions. As a result of the study, the intensified extraction method for Acorus calamus leaves – re-maceration with ultrasound – was established. The conducted researches allowed to develop the method of extraction, expedient in the conditions of the modern pharmaceutical industry.

Author Biographies

Oleksiy Andryushayev, National University of Pharmacy

Postgraduate Student

Department of Industrial Technology of Drugs

Olena Ruban, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor, Head of Department

Department of Industrial Technology of Drugs

Yuliia Maslii, National University of Pharmacy

PhD, Associate Professor

Department of Industrial Technology of Drugs

Inna Rusak, National University of Pharmacy

Leading Specialist

Educational and Scientific Training Laboratory of Chemical and Technological Research

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Published

2021-08-31

How to Cite

Andryushayev, O., Ruban, O., Maslii, Y., & Rusak, I. (2021). Intensification of the extraction process of phenolic compounds from Acorus calamus leaves. ScienceRise: Pharmaceutical Science, (4(32), 4–10. https://doi.org/10.15587/2519-4852.2021.238329

Issue

No. 4(32) (2021)

Section

Pharmaceutical Science

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Copyright (c) 2021 Oleksiy Andryushayev, Olena Ruban, Yuliia Maslii, Inna Rusak

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