Antinociceptive effects of Treculia africana decne (african breadfruit) seed lectin in Wistar rats

Authors

DOI:

https://doi.org/10.15587/2519-4852.2022.270312

Keywords:

hemagglutinin, agglutinin, analgesic activity, pain-relieving lectin, inflammation, Moraceae

Abstract

The aim: The use of synthetic compounds to treat many diseases must be strictly controlled due to their potential health hazards. Hence, there is a need to search for natural products to serve as safe alternatives to synthetic products. This study investigated the antinociceptive effects and anti-inflammatory activities of Treculia africana seed lectin.

Materials and methods: Lectins were purified from Treculia africana seeds using ion exchange and size-exclusion chromatography. The antinociceptive activity of the lectin was assessed in Wistar rats using abdominal writhing and paw-licking tests induced by acetic acid and formalin, respectively. Anti-inflammatory activity was assessed using carrageenan-induced paw oedema.

Results: Treculia africana seed lectins at 10 mg/kg (p.o.) produced sedation, reduced ambulation, reduced response to touch, analgesia, and decreased defecation in experimental animals. Administration of Treculia africana seed lectin (1 mg/kg and 10 mg/kg) in experimental animals significantly reduced (P < 0.05) acetic acid-induced muscular writhing in a dose-dependent manner with 23.88 and 36.80 per cent inhibition, respectively. Both early and late phases of formalin-induced nociception were significantly inhibited (P < 0.001) by the lectin at all doses (0.1, 1.0 and 10.0 mg/kg), comparably with the standard drug, diclofenac sodium. At 10 mg/kg, T. africana lectin caused a 69.12 % and 65.55 % reduction in both early and late phases of formalin-induced paw licking. Treculia africana lectin also significantly brought about a reduction (P < 0.05) in inflammation induced by sub-plantar injection of carrageenan as measured by a decrease in paw swollenness.

Conclusion: The study showed that Treculia africana lectin possesses antinociceptive and anti-inflammatory properties and can potentially be employed therapeutics to ameliorate pain and inflammation

Author Biographies

Joseph Obabiolorunkosi Awe, Afe Babalola University

MSc Biochemistry

Department of Chemical Sciences

Olukemi Adetutu Osukoya, Afe Babalola University

PhD Biochemistry

Department of Chemical Sciences

Olusola Bolaji Adewale, Afe Babalola University

PhD Biochemistry

Department of Chemical Sciences

Tajudeen Olabisi Obafemi, Afe Babalola University

PhD Biochemistry

Department of Chemical Sciences

Olakunle Bamikole Afolabi, Afe Babalola University

PhD Biochemistry

Department of Chemical Sciences

Adenike Kuku, Obafemi Awolowo University

PhD Biochemistry

Department of Biochemistry and Molecular Biology

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Published

2022-12-30

How to Cite

Awe, J. O., Osukoya, O. A., Adewale, O. B., Obafemi, T. O., Afolabi, O. B., & Kuku, A. (2022). Antinociceptive effects of Treculia africana decne (african breadfruit) seed lectin in Wistar rats. ScienceRise: Pharmaceutical Science, (6(40), 43–50. https://doi.org/10.15587/2519-4852.2022.270312

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No. 6(40) (2022)

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Pharmaceutical Science

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Copyright (c) 2022 Joseph Obabiolorunkosi Awe, Olukemi Adetutu Osukoya, Olusola Bolaji Adewale, Tajudeen Olabisi Obafemi, Olakunle Bamikole Afolabi, Adenike Kuku

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