Two masterful alternative spectrophotometric methods for the determination of ramipril in tablets

Authors

DOI:

https://doi.org/10.15587/2519-4852.2024.302635

Keywords:

ramipril, spectrophotometry, validation, quantitative determination, tablets

Abstract

The aim of the work was to develop two simple, rapid, economic, alternative spectrophotometric methods for the determination of ramipril in tablets based on the reaction with sulfonephthalein dyes (bromphenol blue (BPB) and cresol red (CR)).

Materials and methods. Analytical instrumentation: Shimadzu UV-1800 double beam UV-VIS spectrophotometer (Japan) with attached UV-Probe ver. 2.62 software, RAD WAG AS 200/C precise analytical balance (Poland). Ramipril (purity ≥98 % (HPLC)) was purchased from AARTI Industries Limited (India). Ramipril tablets 5 mg and 10 mg were purchased from a local pharmacy.

Results and discussion. Two spectrophotometric methods for the determination of ramipril in tablets have been developed. We have tested various sulfophthalein dyes (BPB, bromocresol green, bromthymol blue, thymol blue, CR) in order to choose the optimal for the method development. According to the results of the experimental studies, we chose BPB and CR as reagents, and the solvent was acetonitrile for both methods. The optimal conditions for the quantitative determination of ramipril in tabletsy using BPB were established: dye concentration - 2.35×10-4 mol/L, volume of BPB solution – 1.0 ml, without heating, wavelength – 598 nm, reaction time – 5 min, temperature of the solution – 25 °C. The optimal conditions for the quantitative determination of ramipril in tabletsusing CR were established: dye 1.33×10-4 mol/L, volume of CR solution – 1.0 ml, without heating, wavelength – 395 nm, reaction time – 5 min, temperature of the solution – 25 °C. The spectrophotometric method by using BPB was linear in the concentration range of 1.99-5.96 µg/mL, LOD – 0.20 µg/mL, LOQ – 0.60 µg/mL. The spectrophotometric method using CR was linear in the concentration range of 0.42-5.44 µg/mL, LOD – 0.10 µg/mL, and LOQ – 0.36 µg/mL. The results of the study on robustness, accuracy, and precision were within the acceptance criteria. The results of studying the «greenness» of both methods indicate an excellent «green» analysis.Conclusions. Developed methods can be used as an alternative method for the routine analysis of ramipril tablets

Author Biographies

Kateryna Typlynska, I. Horbachevsky Ternopil National Medical University; JSC «Farmak»

Postgraduate Student

Department of Pharmaceutical Chemistry

 

Mariana Horyn, I. Horbachevsky Ternopil National Medical University

PhD, Assistant

Department of Pharmaceutical Chemistry

Tetyana Kucher, I. Horbachevsky Ternopil National Medical University

PhD, Associate Professor

Department of Pharmaceutical Chemistry

Liubomyr Kryskiw, I. Horbachevsky Ternopil National Medical University

PhD, Associate Professor

Department of Pharmaceutical Chemistry

Liliya Logoyda, I. Horbachevsky Ternopil National Medical University

Doctor of Pharmaceutical Sciences, Professor, Head of Department

Department of Pharmaceutical Chemistry

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Two masterful alternative spectrophotometric methods for the determination of ramipril in tablets

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Published

2024-04-30

How to Cite

Typlynska, K., Horyn, M., Kucher, T., Kryskiw, L., & Logoyda, L. (2024). Two masterful alternative spectrophotometric methods for the determination of ramipril in tablets. ScienceRise: Pharmaceutical Science, (2 (48), 44–52. https://doi.org/10.15587/2519-4852.2024.302635

Issue

No. 2 (48) (2024)

Section

Pharmaceutical Science

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Copyright (c) 2024 Kateryna Typlynska, Mariana Horyn, Tetyana Kucher, Liubomyr Kryskiw, Liliya Logoyda

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