Research in component composition of the volatile fractions from the genus Anemone plants

Authors

DOI:

https://doi.org/10.15587/2519-4852.2025.323994

Keywords:

Anemone nemorosa L., Anemone ranunculoides L., Ranunculaceae, essential oil, herb, rhizomes, component composition, GC/MS

Abstract

Plants of the genus Anemone L. of the Ranunculaceae family are perennial herbaceous plants. The genus includes about 200 species, five of which are growing on the territory of Ukraine. Despite their relatively wide distribution and availability of raw materials, plants of this genus remain insufficiently studied.

The aim. The aim of the work was to conduct comparative research on the volatile substances in A. nemorosa L. and A. ranunculoides L. raw materials harvested in Ukraine.

Materials and Methods. Hydrodistillates obtained from dried herb and rhizomes of A. nemorosa L. and dried herb of A. ranunculoides L. were analyzed using gas chromatography-mass spectrometry (GC/MS).

Research results. The investigation identified 68 and 50 compounds in A. nemorosa and A. ranunculoides raw materials, respectively. The key classes of organic compounds in the compositions of volatile fractions of the studied samples are: aldehydes (30.9 – 42.4 %), monoterpenoids (6.1 – 15.5 %), organic acids (4.2 – 15.8 %), aliphatic and aromatic hydrocarbons (0 – 8.7 %), sesquiterpenoids (7.9 – 18.9 %), diterpenoids (1.9 – 6.1 %), esters (1.8 – 4.4 %), aromatic compounds (4.4 – 7.9 %), alcohols (4.4 – 9.1 %) and ketones (4.4 – 9.1 %). The main components of the volatile fractions of A. nemorosa were identified as hexahydrofarnesyl acetone, dibutyl phthalate, phytol, hexanal, 2-pentylfuran, and (E)-β-ionone. For A. ranunculoides, the basic compounds were hexahydrofarnesyl acetone, n-hexadecanoic acid, phytol, phthalic acid, and pelargonaldehyde.

Conclusions. The component composition of volatile fractions in 11 samples of Anemone L. species from the flora of Ukraine was analyzed using the GC/MS method. It has been found that the A. nemorosa rhizomes contain the highest level of volatile fraction. The analysis of the component composition of volatile fractions in Anemone species indicates the prospects for further research in this plant species, their pharmacological activities, and their applications

Supporting Agency

  • This work was supported by the European Union in the MSCA4Ukraine project “Design and development of 3D-printed medicines for bioactive materials of Ukrainian and Estonian medicinal plants origin” [ID number 1232466]. Views and opinions expressed are, however, those of the author(s) only and do not necessarily reflect those of the European Union. Neither the European Union nor the MSCA4Ukraine Consortium as a whole nor any individual member institutions of the MSCA4Ukraine Consortium can be held responsible for them.

Author Biographies

Liliia Tuzin, Ivano-Frankivsk National Medical University

Assistant

Department of Pharmaceutical Management, Drug Technology and Pharmacognosy

Andriy Grytsyk, Ivano-Frankivsk National Medical University

Doctor of Pharmaceutical Sciences, Professor, Head of Department

Department of Pharmaceutical Management, Drug Technology and Pharmacognosy

Thanh Tung Nguyen, Hanoi University of Pharmacy

PhD, Lecturer

Department of Pharmacognosy

Ain Raal, University of Tartu

PhD, Professor

Institute of Pharmacy

Oleh Koshovyi, National University of Pharmacy; University of Tartu

Doctor of Pharmaceutical Sciences, Professor

Department of Pharmacognosy;

Institute of Pharmacy

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Research in component composition of the volatile fractions from the genus Anemone plants

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Published

2025-02-28

How to Cite

Tuzin, L., Grytsyk, A., Nguyen, T. T., Raal, A., & Koshovyi, O. (2025). Research in component composition of the volatile fractions from the genus Anemone plants. ScienceRise: Pharmaceutical Science, (1 (53), 106–114. https://doi.org/10.15587/2519-4852.2025.323994

Issue

No. 1 (53) (2025)

Section

Pharmaceutical Science

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Copyright (c) 2025 Liliia Tuzin, Andriy Grytsyk, Thanh Tung Nguyen, Ain Raal, Oleh Koshovyi

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