The usage of salts of chaotropic anions for the development of HPLC method for the simultaneous determination of cisplatin and carboplatin in model mixture
DOI:
https://doi.org/10.15587/2519-4852.2025.341476Keywords:
platinum-based drugs, high-performance liquid chromatography, assay, validation, greennessAbstract
The aim of the work was to develop an RP-HPLC method for the simultaneous determination of extremely polar and non-UV-absorbing chromophore molecules of cisplatin and carboplatin in a model mixture using salts of chaotropic anions, which can be used for single-analyte determination too.
Material and methods: HPLC analysis was performed using Agilent 1260 and Shimadzu LC-2050C with diode array detector (DAD). Cisplatin and carboplation (purity ≥99% (HPLC)) were supplied from Sigma-Aldrich Chemicals Co. (St. Louis, MO, USA). Used dosage forms: cisplatin «Ebeve» (0.5 mg/ml, Austria) and carboplatin «Ebeve» (10 mg/ml, Austria). All used reagents were HPLC gradient chromatography quality and purchased from Merck Darmstadt, Germany.
Results and discussion: Chaotropic agents enhance retention of basic molecules in acidic mobile phases on reversed-phase chromatographic columns and improve peak shape and symmetry. The chaotropic anions that increase interaction between the basic N-containing analyte and the alkyl chains of reversed-phase ligands, such as C-8 and C-18, are frequently employed to enhance and improve the performance of HPLC methods. The chromatographic analysis of cisplatin and carboplatin presented a unique challenge due to their inorganic structure. The experimentally established optimal chromatographic conditions are: mobile phase - 40 mM KPF6 buffer solution (pH 2.4) and ACN (95:5), chromatographic column - Luna C18 (100 x 4.6 mm 3 µm), column temperature - 30 °C, flow rate - 0.4 ml/min, detection wavelength - 210 nm. Linearity was assessed using five levels of each of the investigated drugs, where concentration varied in the range of 20–100 μg/mL. The proposed HPLC method is green, as confirmed by the most modern metrics for studying greenness (AGREE, MoGAPI, complex MoGAPI, AGSA, CaFRI and CACI).
Conclusions. In this work, thorough scientific research was carried out with the presentation of HPLC method development for the simultaneous determination of extremely highly polar molecules cisplatin and carboplatin in a model mixture using salts of chaotropic anions. In addition, two studied drugs were quantified using rapid, simple, cost-effective HPLC method approaches
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Copyright (c) 2025 Mariana Druchok, Marjan Piponski, Mariana Horyn, Nadiya Zarivna, Liliya Logoyda
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