Genetic variability of SARS-CoV-2 virus in the inter-peak period

Authors

  • V Myasoedov Kharkiv National Medical University of the Ministry of Health of Ukraine, Ukraine
  • P Nartov Municipal Nonprofit Enterprise of Kharkiv Regional Council “Regional Clinical Infectious Hospital”, Kharkiv, Ukraine., Ukraine
  • K Yurko Kharkiv National Medical University of the Ministry of Health of Ukraine, Ukraine
  • V Lisovyi Kharkiv National Medical University, Ukraine
  • V Kapustnyk Kharkiv National Medical University of the Ministry of Health of Ukraine, Ukraine
  • A Bondarenko Kharkiv National Medical University of the Ministry of Health of Ukraine, Ukraine
  • М Cherniak Kharkiv National Medical University, Ukraine
  • V Yakushchenko National Pharmaceutical University, Ukraine
  • V Maslova Medical Work of Municipal Nonprofit Enterprise of Kharkiv Regional Council “Regional Clinical Infectious Hospital”, Kharkiv, Ukraine., Ukraine
  • O Bondarenko Municipal Nonprofit Enterprise of Kharkiv Regional Council “Regional Clinical Infectious Hospital”, Kharkiv, Ukraine., Ukraine

Abstract

The aim of the study – to determine the variants of SARS-CoV-2 virus in the inter-peak period (before the beginning of the new wave) in patients of Kharkiv and Kharkiv region with confirmed SARS-CoV-2 infection. Materials and methods. The smears (40 nasopharyngeal samples) from patients with symptoms of ARVI or suspected COVID-19, who were hospitalized to MNE KRC "Regional clinical infectious diseases hospital" in June 2021 and who were identified as positive for SARS-CoV-2 were used study for research conducting. Cross-sectional study of the detection of variants of SARS-CoV-2 circulating in the Kharkiv region in June 2021 was conducted. According to the method of systematic probabilistic sampling for the study of genetic variability of SARS-COV-2, 40 patients with COVID-19 were selected. Among the studied patients, females predominated – 25 people against 15, respectively. The average age of patients was 60.4 years (minimum - 25 years, maximum - 91 years). All patients were hospitalized in the presence of respiratory failure, but community-acquired pneumonia was visualized instrumentally (computed tomography, radiography, ultrasound) in 38 patients. Clinical group III was established in 21 patients, clinical group IV – in 17. Mortality among the studied group was 15%. Research results. The predominant circulation of variant 201/501Y.V1 (alpha) was detected during PCR studies on variants of SARS-CoV-2 virus circulating in Kharkiv and the Kharkiv region in the inter-peak period (before the beginning of the third wave). Alpha variant was detected in 35 patients (87.5%) with typical for this virus mutation – N501Y, but the presence of additional mutations was identified in 1 patient. The original SARS-CoV-2 virus was detected in 5 patients (12.5%), but in 1 of them with the presence of the N501Y mutation. Circulation of only variant 201/501Y.V1 was detected in all residents of the region. Circulation of SARS-CoV-2 variants 20H/501Y.V2 (beta), 20J/501Y.V3 (gamma), 20C/S.452R (previously epsilon) was not detected in Kharkiv and the region, and the E484K mutation was not found in any patient.

Conclusions. The dominant variant circulating in Kharkiv and the region in the inter-peak period is the alpha variant, which prevails over the original virus due to the increased level of contagiousness and more rapid spread.

Key words: COVID-19, SARS-COV-2, genetic variability.

DOI: 10.5281/zenodo.6350409

Author Biographies

V Myasoedov , Kharkiv National Medical University of the Ministry of Health of Ukraine

Vice-Rector for Research of Kharkiv National Medical University, Professor, Doctor of Medical Science, Honored Worker of Science and Technology of Ukraine.

P Nartov , Municipal Nonprofit Enterprise of Kharkiv Regional Council “Regional Clinical Infectious Hospital”, Kharkiv, Ukraine.

Director of Municipal Nonprofit Enterprise of Kharkiv Regional Council “Regional Clinical Infectious Hospital”, Kharkiv, Ukraine.

K Yurko, Kharkiv National Medical University of the Ministry of Health of Ukraine

Department of Infectious Diseases of Kharkiv National Medical University, Kharkiv, Ukraine.

V Lisovyi , Kharkiv National Medical University

The Head of Academic Council of Kharkiv National Medical University, Corresponding member of the National Academy of Medical Sciences of Ukraine, Doctor of Medical Science, winner of State Prize of Ukraine, Professor, Kharkiv, Ukraine.

V Kapustnyk , Kharkiv National Medical University of the Ministry of Health of Ukraine

rector of Kharkiv National Medical University, Doctor of Medical Science, Professor, Honored Educator of Ukraine, Kharkiv

A Bondarenko , Kharkiv National Medical University of the Ministry of Health of Ukraine

Doctor of Medical Science, Professor of the Department of Infectious Diseases of Kharkiv National Medical University, Kharkiv, Ukraine.

М Cherniak , Kharkiv National Medical University

Doctor of Philosophy, Assistant of the Department of Community Health and Health Protection Management of Kharkiv National Medical University, Kharkiv, Ukraine.

V Yakushchenko , National Pharmaceutical University

Candidate of Pharmaceutical Sciences, Associate Professor of the Department of Foreign Pharmacy and Security of IPC (Institute for the Development of Qualifications of Specialties) of the National Pharmaceutical University, Kharkiv, Ukraine.

V Maslova , Medical Work of Municipal Nonprofit Enterprise of Kharkiv Regional Council “Regional Clinical Infectious Hospital”, Kharkiv, Ukraine.

Deputy Chief Physician for Medical Work of Municipal Nonprofit Enterprise of Kharkiv Regional Council “Regional Clinical Infectious Hospital”, Kharkiv, Ukraine.

O Bondarenko , Municipal Nonprofit Enterprise of Kharkiv Regional Council “Regional Clinical Infectious Hospital”, Kharkiv, Ukraine.

supervisor of clinical and diagnostic laboratory Municipal Nonprofit Enterprise of Kharkiv Regional Council “Regional Clinical Infectious Hospital”, Kharkiv, Ukraine.

Published

2022-03-15

How to Cite

Myasoedov , V., Nartov , P., Yurko, K., Lisovyi , V., Kapustnyk , V., Bondarenko , A., Cherniak М., Yakushchenko , V., Maslova , V., & Bondarenko , O. (2022). Genetic variability of SARS-CoV-2 virus in the inter-peak period. Annals of Mechnikov’s Institute, (1), 42–45. Retrieved from http://journals.uran.ua/ami/article/view/246394

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Section

Articles