Devising an express method for estimating the quality of colostrum and its components based on electrical conductivity

Authors

DOI:

https://doi.org/10.15587/1729-4061.2021.225007

Keywords:

electrical conductivity, colostrum, biologically active compounds, low-molecular proteins, lipids, temperature, storage

Abstract

This paper reports the development of an express method for assessing the quality of biologically active substances derived from colostrum. We tested the hypothesis that there may be a dependence between the amount of protein that is part of the colostrum and its characteristic (a difference in molecular masses) and electrical conductivity.

It has been shown that the colostrum contains several hundred proteins: it depends on the individual characteristics of cattle. The removal of lipids was accompanied by an increase in electrical conductivity from 5 % to 18 % compared to the whole colostrum while the subsequent removal of high-molecular proteins increased the electrical conductivity by 50‒100 % compared to skimmed colostrum: this depends on the individual characteristics of cattle. Such an individual feature of the colostrum composition reflects the uniqueness of the individual animal's metabolism. A mathematical model has been built for the dependence of the content of charged molecules in the solution of proteins on the molecular mass of proteins, which explains the relationship between electrical conductivity and the molecular mass of proteins.

It was shown that there is a direct correlation between the colostrum electroconductivity and the temperature in a measuring cell in the range of temperatures from 14 °C to 19 °C. The electrical conductivity of colostrum components increased by no more than 20 % during storage (at a temperature of 3‒4 °C) up to 18 days, which is associated with protein degradation The electrical conduction method could be used to assess the colostrum composition during storage.

Technology for obtaining different colostrum components (skimmed fraction and a fraction of low-molecular components) has been devised, as well as a method for assessing the quality of products based on the characteristics of electrical conductivity.

Electrical conductivity is a promising method for assessing the quality of products that are derived from colostrum, at different shelf life at different stages of production: raw materials, fat removal, obtaining a fraction with a predefined composition of proteins

Author Biographies

Valentyn Kozheshkurt, V. N. Karazin Kharkiv National University

Researcher

Department of Physical and Biomedical Electronics and Complex Information Technologies

Ievgen Ivanov , «Alpha» Farm Enterprise

PhD, Commercial Director

Yevhenii Antonenko, V. N. Karazin Kharkiv National University

Senior Lecturer, Researcher

Department of Physical and Biomedical Electronics and Complex Information Technologies

Victor Katrich, V. N. Karazin Kharkiv National University

Doctor of Physical and Mathematical Sciences, Professor

Department of Physical and Biomedical Electronics and Complex Information Technologies

Anatoly Bozhkov, V. N. Karazin Kharkiv National University

Doctor of Biological Sciences, Professor

Department of Molecular Biology and Biotechnology

Taras Gromovoy , Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine

PhD, Senior Researcher

Laboratory of Mass Spectrometry of Surface of Nanosystems

References

  1. Li, M., Li, Q., Kang, S., Cao, X., Zheng, Y., Wu, J. et. al. (2020). Characterization and comparison of lipids in bovine colostrum and mature milk based on UHPLC-QTOF-MS lipidomics. Food Research International, 136, 109490. doi: https://doi.org/10.1016/j.foodres.2020.109490
  2. Vetvicka, V., Vetvickova, J. (2019). Effects of Transfer Factor Supplementation on Immune Reactions in Mice. Journal of Nutrition and Health Sciences, 6 (3), 301.
  3. Borad, S. G., Singh, A. K. (2018). Colostrum immunoglobulins: Processing, preservation and application aspects. International Dairy Journal, 85, 201–210. doi: https://doi.org/10.1016/j.idairyj.2018.05.016
  4. Bozhkov, A. I., Nikitchenko, Y. V., Lebid, K. M., Ivanov, E. G., Kurguzova, N. I., Gayevoy, S. S., Al Begai M. A. Y. (2017). Low molecular weight components from various sources eliminate oxidative stress and restore physiological characteristic of animals at early stages of Cu- induced liver fibrosis development. Translational Biomedicine, 8 (2). doi: https://doi.org/10.21767/2172-0479.1000107
  5. Bozhkov, A. I., Ivanov, E. G., Begai, M., Alsardia, M., Kurguzova, N. I. (2017). Low-Molecular Weight Cow Colostrum Components in Functional Nutrition. Journal of Nutritional Therapeutics, 6 (1), 11–17. doi: https://doi.org/10.6000/1929-5634.2017.06.01.2
  6. Ascher, M. S., Gottlieb, A. A., Kirkpatrick, C. H. (Eds.) (1976). Transfer factor: Basic properties and clinical applications. Academic Press, 780. doi: https://doi.org/10.1016/c2013-0-07152-6
  7. Berkowitz, M., Wan, W. (1987). The limiting ionic conductivity of Na+and Cl−ions in aqueous solutions: Molecular dynamics simulation. The Journal of Chemical Physics, 86 (1), 376–382. doi: https://doi.org/10.1063/1.452574
  8. Becker, F. F., Wang, X. B., Huang, Y., Pethig, R., Vykoukal, J., Gascoyne, P. R. (1995). Separation of human breast cancer cells from blood by differential dielectric affinity. Proceedings of the National Academy of Sciences, 92 (3), 860–864. doi: https://doi.org/10.1073/pnas.92.3.860
  9. Rosenberg, B., Jendrasiak, G. L. (1968). Semiconductive properties of lipids and their possible relationship to lipid bilayer conductivity. Chemistry and Physics of Lipids, 2 (1), 47–54. doi: https://doi.org/10.1016/0009-3084(68)90034-0
  10. Hagiwara, K., Kataoka, S., Yamanaka, H., Kirisawa, R., Iwai, H. (2000). Detection of cytokines in bovine colostrum. Veterinary Immunology and Immunopathology, 76 (3-4), 183–190. doi: https://doi.org/10.1016/s0165-2427(00)00213-0
  11. Sinanoglou, V. J., Cavouras, D., Boutsikou, T., Briana, D. D., Lantzouraki, D. Z., Paliatsiou, S. et. al. (2017). Factors affecting human colostrum fatty acid profile: A case study. PLOS ONE, 12 (4), e0175817. doi: https://doi.org/10.1371/journal.pone.0175817
  12. Puppel, K., Gołębiewski, M., Grodkowski, G., Slósarz, J., Kunowska-Slósarz, M., Solarczyk, P. et. al. (2019). Composition and Factors Affecting Quality of Bovine Colostrum: A Review. Animals, 9 (12), 1070. doi: https://doi.org/10.3390/ani9121070
  13. Elfstrand, L., Lindmark-Månsson, H., Paulsson, M., Nyberg, L., Åkesson, B. (2002). Immunoglobulins, growth factors and growth hormone in bovine colostrum and the effects of processing. International Dairy Journal, 12 (11), 879–887. doi: https://doi.org/10.1016/s0958-6946(02)00089-4
  14. Sánchez-González, D. J., Sosa-Luna, C. A., Vásquez-Moctezuma, I. (2011). Factores de transferencia en la terapéutica médica. Medicina Clínica, 137 (6), 273–277. doi: https://doi.org/10.1016/j.medcli.2010.05.002
  15. Mesmin, C., Fenaille, F., Becher, F., Tabet, J.-C., Ezan, E. (2011). Identification and Characterization of Apelin Peptides in Bovine Colostrum and Milk by Liquid Chromatography–Mass Spectrometry. Journal of Proteome Research, 10 (11), 5222–5231. doi: https://doi.org/10.1021/pr200725x
  16. Kozheshkurt, V., Antonenko, Y., Shtoda, D., Slipchenko, O., Katrych, V. (2018). Possibilities of Impedance Spectroscopy for the Study of Bioliquids. 2018 9th International Conference on Ultrawideband and Ultrashort Impulse Signals (UWBUSIS). doi: https://doi.org/10.1109/uwbusis.2018.8520236
  17. Qian, X., Gu, N., Cheng, Z., Yang, X., Wang, E., Dong, S. (2001). Methods to study the ionic conductivity of polymeric electrolytes using a.c. impedance spectroscopy. Journal of Solid State Electrochemistry, 6 (1), 8–15. doi: https://doi.org/10.1007/s100080000190
  18. Maskow, T., Röllich, A., Fetzer, I., Ackermann, J.-U., Harms, H. (2008). On-line monitoring of lipid storage in yeasts using impedance spectroscopy. Journal of Biotechnology, 135 (1), 64–70. doi: https://doi.org/10.1016/j.jbiotec.2008.02.014
  19. Lowry, O., Rosebrough, N., Farr, A. L., Randall, R. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193 (1), 265–275. doi: https://doi.org/10.1016/s0021-9258(19)52451-6
  20. Gramse, G., Dols-Perez, A., Edwards, M. A., Fumagalli, L., Gomila, G. (2013). Nanoscale Measurement of the Dielectric Constant of Supported Lipid Bilayers in Aqueous Solutions with Electrostatic Force Microscopy. Biophysical Journal, 104 (6), 1257–1262. doi: https://doi.org/10.1016/j.bpj.2013.02.011
  21. Gómez Vera, J., Chávez Sánchez, R., Flores Sandoval, G., Orea Solano, M., López Tiro, J. J., Santiago Santos, A. D. et. al. (2010). Transfer factor and allergy. Revista alergia Mexico, 57 (6), 208–214. Available at: https://www.scopus.com/record/display.uri?eid=2-s2.0-84979819260&origin=inward&txGid=8315279df09e01107c79deb948eab9cb#
  22. Rozzo, S. J., Kirkpatrick, C. H. (1992). Purification of transfer factors. Molecular Immunology, 29 (2), 167–182. doi: https://doi.org/10.1016/0161-5890(92)90098-i
  23. D’Amici, G. M., Rinalducci, S., Zolla, L. (2007). Proteomic Analysis of RBC Membrane Protein Degradation during Blood Storage. Journal of Proteome Research, 6 (8), 3242–3255. doi: https://doi.org/10.1021/pr070179d

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Published

2021-02-27

How to Cite

Kozheshkurt, V., Ivanov , I., Antonenko, Y. ., Katrich, V. ., Bozhkov, A. ., & Gromovoy , T. (2021). Devising an express method for estimating the quality of colostrum and its components based on electrical conductivity . Eastern-European Journal of Enterprise Technologies, 1(11 (109), 69–77. https://doi.org/10.15587/1729-4061.2021.225007

Issue

Vol. 1 No. 11 (109) (2021): Technology and Equipment of Food Production

Section

Technology and Equipment of Food Production

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Copyright (c) 2021 Валентин Александрович Кожешкурт , Евгений Геннадьевич Иванов , Евгений Александрович Антоненко , Виктор Александрович Катрич , Анатолий Иванович Божков , Тарас Юрьевич Громовой

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