Syrup Amkesol reduces the level of primary and secondary products of lipid peroxidation and toxic metabolites of nitric oxide in blood serum of immature rats with bronchiolitis
Keywords:
children's dosage forms, syrup Amkesol, bronhoalveolitis, prooxidant-antioxidant system, nitric oxide metabolites.Abstract
Aim of this study was to determine the pharmacological effect of syrup Amkesol evaluating the blood serum levels of primary and secondary products of lipid peroxidation, activity of AO enzymes and final rates of NO metabolites in immature rats of different ages with experimental model of bronchoalveolitis. Materials and Methods: The study was carried out on 90 WAG immature rats of ages 1, 2 and 3 menthes, that correlates by morpho-functional features to 4, 10 and 14 years of human age respectively, on a model of bronchoalveolitis. Experimental animals in each age series were randomly divided into 5 groups (n = 6): intact (healthy), 2 groups of control (untreated with bronchoalveolitis) 7 and 14 days, and two groups with bronchoalveolatis that received S-AKS daily during 7 and 14 days. The pathological process implemented by inhalation of irritant (Sephadex A-25 Pharmacia, Sweden (5 mg/kg)). In the blood serum samples activity of CAT, SOD, content of DC, TBARS, total NO, nitrates and nitrites were determined. Probability of the results was evaluated by using GraphPad Prism Software. The critical level of significance was taken equal to 0.05. Results. The use of S-AKS on the 7th day in the group of 1- and 3-month-old rats significantly exceeded activity of CAT compared to the control group of animals of corresponded age. The SOD activity in group of 2-month-old animals was restored to intact level, the activity of CAT exceeded the baseline level and amounted to 129% (P≤0,05). The concentration of DC in 1-month-old rats was lower by 45.3% in 2 monthly - by 12.5% than in the control group, in the group of 3 month-old animals - restored to normal (P≤0,05). The level of NO metabolites was significantly decreased compared to the corresponding control group in all age series (P≤0,05). After 14 days of treatment with S-AKS in all age group of animals observed restore the contents of primary and secondary products of lipid peroxidation compared to the intact level. Conclusion. Administration of S-AKS reduced the values of primary and secondary products of lipid peroxidation; restored the activity of antioxidant enzymes; reduced the level of NO and its stable metabolites to the physiological level in all studied age groups of animals with experimental bronchoalveolitis.
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