Syrup Amkesol reduces the level of primary and secondary products of lipid peroxidation and toxic metabolites of nitric oxide in blood serum of immature rats with bronchiolitis

Автор(и)

  • K Storozhenko KHNMU, Department of Pharmacology and Drug Prescription,

Ключові слова:

children's dosage forms, syrup Amkesol, bronhoalveolitis, prooxidant-antioxidant system, nitric oxide metabolites.

Анотація

Aim of this study was to determine the pharmacological effect of syrup Amkesol evaluating the blood serum levels of primary and secondary products of lipid peroxidation, activity of AO enzymes and final rates of NO metabolites in immature rats of different ages with experimental model of bronchoalveolitis. Materials and Methods: The study was carried out on 90 WAG immature  rats of ages 1, 2 and 3 menthes, that correlates by morpho-functional features to 4, 10 and 14 years of human age respectively, on a model of bronchoalveolitis. Experimental animals in each age series were randomly divided into 5 groups (n = 6): intact (healthy), 2 groups of control (untreated with bronchoalveolitis) 7 and 14 days, and two groups with bronchoalveolatis that received S-AKS daily during 7 and 14 days.  The pathological process implemented by inhalation of irritant (Sephadex A-25 Pharmacia, Sweden (5 mg/kg)). In the blood serum samples activity of CAT, SOD, content of DC, TBARS, total NO, nitrates and nitrites were determined. Probability of the results was evaluated by using GraphPad Prism Software. The critical level of significance was taken equal to 0.05. Results.  The use of S-AKS on the 7th day in the group of 1- and 3-month-old rats significantly exceeded activity of CAT compared to the control group of animals of corresponded age. The SOD activity in group of 2-month-old animals was restored to intact level, the activity of CAT exceeded the baseline level and amounted to 129% (P≤0,05). The concentration of DC in 1-month-old rats was lower by 45.3% in 2 monthly - by 12.5% than in the control group, in the group of 3 month-old animals - restored to normal (P≤0,05). The level of NO metabolites was significantly decreased compared to the corresponding control group in all age series (P≤0,05). After 14 days of treatment with S-AKS in all age group of animals observed restore the contents of primary and secondary products of lipid peroxidation compared to the intact level. Conclusion. Administration of S-AKS reduced the values of primary and secondary products of lipid peroxidation; restored the activity of antioxidant enzymes; reduced the level of NO and its stable metabolites to the physiological level in all studied age groups of animals with experimental bronchoalveolitis.

Посилання

Mizernickij, YU. L. Prospects for the development of specialized care for children with respiratory diseases // Childhood Pulmonary Medicine: Problems and solutions. 2009 (9), P. 8-17.

Samsygina, G. A. Sickly children: problems of pathogenesis, diagnosis and therapy // Pediatriya, 2005, №1, P. 66-73.

Botvin'eva, E. A. Bronchial obstruction of infectious origin in children. // Med. panorama 2007 №3, P. 49-51.

Lasica, O. I. Current approaches to the treatment of bronchial asthma in children // Ukr. med. chasopis, 1998 №1 (3), P. 14-17.

Novikova N. E., Kudryasheva I. A., Ahmineeva A. H. Oxidative stress in chronic obstructive pulmonary disease // Astrahanskij med. journ., 2012, Vol. 7, (3), P. 87-90.

Bolevich S. B. The value of free-radical processes in asthma (pathogenic, clinical and therapeutic aspects) // Moscow : Izdatelstvo Meditsina, 2006. — 256 s.

Zvyagina, T. V., Anikeeva, T. V., & Belokon', T. M. The clinical significance of changes in the metabolism of nitric oxide in pulmonology // Ukr. pul'monol. zhurnal, 2002 №1, P. 66-68.

Titov, V. YU., Krejnina, M. V., Petrov, V. A. et al. High sensitivity and high specificity inflammation detector. Bulletin of RSMU,2015, №4, P. 51-57.

Macievich, M. V. Endothelial dysfunction, prooxidant and antioxidant system in patients with chronic obstructive pulmonary disease in combination with hypertension in the course of antihypertensive therapy // Russian State Medical University . — Moscow, 2008, 5 p.

Zajceva, O. V. The syndrome of bronchial obstruction in children // Pediatriya, 2005, №4, 94-104.

Zajceva, O. V. Rational choice of mucolytic therapy in the treatment of respiratory diseases in children // Russian med. journal, 2009 Vol.17, №19, P. 1217–1222.

Romancov, M. G., Ershov, F. I. (2006). RRI in children: a modern pharmacotherapy //2006, Moscow: Gehotar-media, 191p.

Baleva, L. S., Korovina, N. A., Tatochenko, V. K. Current approaches to the treatment and rehabilitation of RRI children // 2006, Moscow: Agency of med. marketing, 56 p.

Baranova, A. A. ed. Rational pharmacotherapy of childhood diseases. Guidance for practicing physicians // 2007,Moscow: Litterra, 1146p.

On-line medicines Compendium in Ukrane. URL: http://compendium.com.ua/info/220123/amkesol ( request date 17.07.2016).

Denisova, M. F. , Nіkіtіna N. S., Dzyuba І. P. at al. Pre-clinical study of the safety of medicines intended for use in pediatric patients // 2002, Methodic recommendations, Kiїv, 27sp.

Rybolovlev YU. R., Rybolovlev R.S. Dosing of substances for mammalian by constants of biological activity // ZH. AMN SSSR, 1979 Vol. 247, №6, P. 1513-1516.

Makarova, O. V., Kovaleva, V. L., Sladkopevcev, A. S. et al. The experimental model of non-infectious pulmonary granulomatosis // Pulmonology, 1996, №1, P. 76-79.

Ultrasound inhaler of dry powder medications: pat. 87091 Ukraine. Number 201307740, appl. 18.06.2013; publ. 27.01.2014, Bull. Number 2.

L'vovskaya, E. I., Volchegorskij, I. A., Shemyakov et al. Spectrophotometric determination of the end products of lipid peroxidation // Problems of Medical Chemistry, 1991, Vol.37 №4, P. 92-93.

Fedorova T. K., Korshunova E. T., Larskaya T. S. Reaction with TBA to determine MDA in blood by fluorometry // Laboratory Science, 1983, №3, P. 25-28.

Korolyuk, M. A., Ivanova, L. I., Majorova et al. The method for determining the activity of catalase // Laboratory Science, 1988, №1, P. 16-19.

Kostyuk, V. A., Potapovich, A. I., & Kovaleva, ZH. V A simple and sensitive method of determination of superoxide dismutase activity based on the reaction of quercetin oxidation // Problems of Medical Chemistry, 1990, Vol.36, №2, P. 88-91.

Ovsyannіkova L. M., Chumak A. A., Nosach O. V at al. Methods for evaluation of free radical oxidation and antioxidant system of the organism in clinical practice (Guidelines)]. Kiїv , 2006, 25p.

Golikov, P. P., Nikolaeva, N. YU. The method for determination of nitrite/ nitrates(NOx) in blood serum. Biomeditsinskaya Khimiya, 2004, Vol. 50, №1, P. 79-85.

Motulsky H. Analyzing data with GraphPad Prism: a companion to GraphPad Prism version 3. GraphPad Software //Inc., San Diego. 379p. – 1999.

Joshi, M. S., Ponthier, J. L., & Lancaster, J. R. Cellular antioxidant and pro-oxidant actions of nitric oxide. // Free Radical Biology and Medicine, 1999, Vol.27, №11, P. 1357-1366.

Johnson T. N., Rostami-Hodjegan A., Tucker G. T. Prediction of the clearance of eleven drugs and associated variability in neonates, infants and children //Clinical pharmacokinetics. – 2006. – Т. 45. – №. 9. – С. 931-956.

##submission.downloads##

Як цитувати

Storozhenko, K. (2019). Syrup Amkesol reduces the level of primary and secondary products of lipid peroxidation and toxic metabolites of nitric oxide in blood serum of immature rats with bronchiolitis. Анали Мечниковського Інституту, (1), 54–61. вилучено із https://journals.uran.ua/ami/article/view/190062

Номер

Розділ

Дослідні статті