Quantitative Determination of Phenol Compounds in Sansevieria Hyacinthoides Leaves

Abstract

Introduction. Sansevieria hyacinthoides (L.) Druce is a member of the family Asparagaceae. The plant is widespread in the countries of subtropical and tropical Africa. In Ukraine, it is grown as an ornamental houseplant. Despite the wide prevalence of Sansevieria hyacinthoides, its chemical composition has hardly been studied. Therefore, one of the stages of the comprehensive study of the plant was the quantitative determination of some phenolic compounds in the Sansevieria hyacinthoides leaves. The purpose of the study was to determine the quantitative content of hydroxycinnamic acids, flavonoids and tannins in the Sansevieria hyacinthoides leaves. Five series of raw materials were studied to get statistically significant results. Materials and Methods. Raw materials for research were harvested in 2020 in the Kharkiv region in the botanical garden of the National University of Pharmacy, then were chopped, and dried at the temperature of 40 °C. The quantitative content of hydroxycinnamic acids was determined by the method of absorption spectrophotometry at 525 nm wavelength in terms of chlorogenic acid. The study was carried out according to the State Pharmacopoeia of Ukraine (monograph "Nettle leaves"). The quantitative content of flavonoids was determined by the method of absorption spectrophotometry at 425 nm wavelength in terms of rutin. The study was carried out according to the State Pharmacopoeia of Ukraine (monograph "Sophora buds"). The quantitative content of tannins was determined by the method of absorption spectrophotometry at 760 nm wavelength in terms of pyrogallol. The study was carried out according to the State Pharmacopoeia of Ukraine (monograph "Determination of tannins in medicinal plant raw materials"). Results & Discussion. The quantitative content of hydroxycinnamic acids in terms of chlorogenic acid (1,06 ± 0,02 %), flavonoids in terms of rutin (1,71 ± 0,03 %) and tannins in terms of pyrogallol (1,47 ± 0,02 %) in the Sansevieria hyacinthoides leaves was determined as a result of the conducted researches. Conclusion. The quantitative content of hydroxycinnamic acids, flavonoids and tannins was determined for complete phytochemical study of Sansevieria hyacinthoides leaves. The obtained results can be used to standardize and develop quality control methods for the studied raw materials. In addition, the research results can help to understand the development of a potential pharmacological activity, and they will be taken into account in the elaboration of new medicinal substances based on the studied raw material – the Sansevieria hyacinthoides leaves. 

Keywords: Sansevieria hyacinthoides, hydroxycinnamic acids, flavonoids, tannins.

DOI: 10.5281/zenodo.5499562