Identification and quantitative content of chlorogenic and rosmarinic acids in Centaurea cyanus l. and Centaurea montana l. herbs

Abstract

Introduction. Plants of Centaurea L. genus are used in complex therapy of many diseases, in particular, as anti-inflammatory, antimicrobial, analgesic, reparative, diuretic, hypoglycemic agents. These activities are partially caused by availability of phenolic compounds, in particular, phenolic acids. For example, chlorogenic and rosmarinic acids are known to take part in antioxidant, antimicrobial, anti-inflammatory, hypolipidemic, hypotensive, hepatoprotective, hypoglycemic activities.
Purpose of the present work was identification and quantitative content determination of chlorogenic and rosmarinic acids in herb of Centaurea cyanus L. and Centaurea montana L., which are widely cultivated in Ukraine as decorative plants. Material & methods. The objects of the study were herb of Centaurea cyanus L. and Centaurea montana L. The raw materials were collected during blossoming in Kharkiv and Odessa regions of Ukraine in June-July 2020/2021. Identification and quantitative content of chlorogenic and rosmarinic acids were carried out using the HPLC method. Results & discussion. In Centaurea montana L. herb chlorogenic and rosmarinic acids were identified to the amount of 4.24 ± 0.08 mg/100 g and 7.47 ± 0.15 mg/100 g respectively, in Centaurea cyanus L. only chlorogenic acid was identified to the amount of 1.40 ± 0.02 mg/100 g. Conclusion. For the first time in Centaurea cyanus L. herb and Centaurea montana L. herb collected in Ukraine chlorogenic acid was identified by HPLC method, whereas in Centaurea montana L. herb additionally rosmarinic acid was found. Centaurea montana L. herb accumulated higher quantity of identified acids. The obtained results may be used in the development of medicines and standardization of tested herb.

Keywords: Centaurea cyanus L., Centaurea montana L., chlorogenic acid, rosmarinic acid, HPLC method.

DOI: 10.5281/zenodo.6350032