Changes cytokines polymorphisms in the genes IL-2, IL -4 and IL -10 patients with pulmonary tuberculosis with chemotherapy

Authors

  • D Butov Kharkiv National Medical University,

Keywords:

tuberculosis, polymorphism of the gene, immunity, cytokines, interleukins.

Abstract

It examined 150 patients with newly diagnosed pulmonary tuberculosis (NDTL) and 30 relatively healthy donors. Areas studied genes interleukin (IL)-2 polymorphism T-330G, IL-4 – C-589T та IL-10 – G-1082A and the level of cytokines (IL- 2, IL-4 and IL-10) in venous blood were measured by ELISA method. The survey for the treatment of patients with NDTL , there was a significant increase in IL-2, and decreased IL-4, IL-10 performance, when compared with relatively healthy donors. After the two-month standard therapy showed a significant reduction in IL-2 and IL-4 content and IL-10 significantly increased. The low level of secretion of IL-4, IL-10 and high IL-2 changes significantly associated with mutant homozygotes C-589T polymorphism of the gene IL- 4, G-1082A – IL-10 and T-330G – IL-2 in patients with infiltrative tuberculosis lungs. IL-4, IL-2 and IL-10 are immune markers of treatment outcomes and can help bring out the best strategy for treating patients with NDTL.  Immunogenetic factors have a protective effect in patients with NDTL is normal homozygous variant promoter regions of C-589T the gene IL-4, G-1082A – IL-10 and T-330 – GIL-2.

References

Cytokine genes are associated with tuberculin skin test response in a native Brazilian population [Text] / V.M. Zembrzuski, P.C. Basta, S.M. Callegari-Jacques [et al.] // Tuberculosis (Edinb). – 2010. – №90. – P. 44-49.

Innate immune recognition of Mycobacterium tuberculosis [Text] / J. Kleinnijenhuis, M. Oosting, L.A. Joosten [et al.] // Clin. Dev. Immunol. – 2011. – 405310.

Hill AV The genomics and genetics of human infectious disease susceptibility [Text] / A.V. Hill // Annu Rev Genomics Hum Genet. – 2001. – №2. – P.373-400.

Moller, M. Current findings, challenges and novel approaches in human genetic susceptibility to tuberculosis [Text] / M. Moller, E.G. Hoal // Tuberculosis (Edinb). – 2010. – №90. – P.71–83.

Vannberg, F.O. Human genetic susceptibility to intracellular pathogens [Text] / F.O. Vannberg, S.J. Chapman, A.V. Hill // Immunol. Rev. – 2011. – №240 – P.105-116.

Knight, J. Polymorphisms in Tumor Necrosis Factor and Other Cytokines As Risks for Infectious Diseases and the Septic Syndrome [Text] / J. Knight // Curr. Infect. Dis. – 2001. – №3. – P. 427-439.

Features of specific immune response in the patients with fibrous/cavernous tuberculosis of lungs [Text] / T.E.Kissina, I.S.Freidlin, B.E. Knoring [et al.] // Med. Immunol. – 2006. – Vol.8, №4. – P. 501-510.

Randomized trial of adjunctive interleukin-2 in adults with pulmonary tuberculosis [Text] / J.L. Johnson, E. Ssekasanvu , A. Okwera [et al.]//Am. J. Respir. Crit. Care Med. – 2003. – Vol.168, №2. – P. 185-191.

Kadagidze, Z.G. Cytokines [Text] / Z.G. Kadagidze // Practical Oncology. – 2003. – Vol.4, № 3. – P. 131-139.

Berezhnaya, N.M., Chekhun V.F. Immunology of malignant growth [Text] // К.: Naukova Dumka. – 2005. – 791 p.

Rook, G.A. Th2 cytokines in susceptibility to tuberculosis [Text] / G.A. Rook // Curr. Mol. Med. – 2007. – Vol. 7, №3. – P.327 - 337.

Regulation and functions of the IL-10 family of cytokines in inflammation and disease [Text] / W. Ouyang, S. Rutz, N.K. Crellin, P.A. Valdez, S.G. Hymowitz // Annu Rev. Immunol. – 2011. – №29. – P.71-109.

Attenuation of cytokine responsiveness during T cell development and differentiation [Text] / J.H. Marino, C.J. Wiele, I. M. Everhart [et al.] // J. Interferon Cytokine Res. – 2006. – V. 26, № 10. – P.748-759.

Lapach, S.N. Statistical methods in biomedical studies using Excel [Text] / Lapach S.N., Chubenko A.V., Babich P.N. – Kyiv: Morion, 2000. – 320 p.

Probability theory and mathematical statistics: Textbook for universities [Text] / Gmurman V.E. – Moscow: Vush. school. 2003. – 479p.

Imangulova, M.M. Molecular genetic aspects of pulmonary tuberculosis [Text] / M.M. Imangulova, A.S. Karunas, E.K. Khusnutdinova / / Medical Genetics.– 2005. – №11. – Р. 505-510.

Allelic polymorphism of cytokine genes during pulmonary tuberculosis [Text] / I.O. Naslednikova, O.I. Urazova , O.V.Voronkova [et al.]// Bull. Exp. Biol. Med. – 2009. – №2 – Р.175-180.

rs2243268 and rs2243274 of Interleukin-4 (IL-4) gene are associated with reduced risk for extrapulmonary and severe tuberculosis in Chinese Han children [Text] / H. Qi, L. Sun , Y.Q. Jin [et al.]// Infect. Genet. Evol. – 2014. – Р. 121-128.

Human cytokine gene nucleotide sequence alignments [Text] / J.L. Bidwell, N.A.P. Wood, H.R. Morse [et al.] // Eur. J. Immunogenet. – 1998. – Vol.25. – P. 83-266.

Impact of IFN-γ and IL10 gene polymorphisms on the synthesis of cytokines in children and adolescents with pulmonary tuberculosis [Text] / A.L. Pospelov, M.M. Averbakh, M.F. Gubkina [et al.] // Tuberculosis and Lung Disease. – 2011. – №3. – Р.52-56.

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How to Cite

Butov, D. (2020). Changes cytokines polymorphisms in the genes IL-2, IL -4 and IL -10 patients with pulmonary tuberculosis with chemotherapy. Annals of Mechnikov’s Institute, (2), 74–78. Retrieved from https://journals.uran.ua/ami/article/view/208014

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Research Articles