Sensitivity to antibacterial drugs in agents of community-acquired infections

Автор(и)

  • T Osolodchenko Mechnikov Institute of Microbiology and Immunology,
  • I Andreieva Mechnikov Institute of Microbiology and Immunology,
  • N Zavada Mechnikov Institute of Microbiology and Immunology,
  • T Lukyanenko Mechnikov Institute of Microbiology and Immunology,
  • O Batrak Mechnikov Institute of Microbiology and Immunology,
  • I Ryabova Mechnikov Institute of Microbiology and Immunology,

Ключові слова:

microorganisms, antibacterial drugs, antibiotic resistance.

Анотація

Introduction The widespread and uncontrolled use of antibiotics leads to selection of resistant strains and rise to atypical forms of most infectious agents. Constantly progressive resistance of microorganisms is currently the most important negative phenomenon of antibiotic therapy. The aim of the work was to optimize antibiotic therapy in community-acquired infections. The objective of study was to determine the range and degree of resistance in clinical isolates of microorganisms of different taxonomic groups, obtained from patients in outpatient care.
Material and methods 213 clinical isolates of microorganisms obtained from patients in outpatient care were studied: 34,7 % strains were obtained from patients with inflammatory processes in upper respiratory tract, 11,7 % – with ear inflammation, 36,6 % – with inflammatory diseases of urinary and genital tracts, 11,3 % – with of skin and soft tissues inflammation, 4,2 % – eye inflammation and 1,4 % – with postoperative infectious complications. The collection of clinical material was performed accordingly before the start of antibacterial therapy. Microorganisms’ isolation and identification were carried out with the help of microbiological methods according to the regulatory documents. The study of resistance of bacterial strains to the antibacterial drugs was performed with the help of disc diffusion method on the Muller-Hinton nutritional medium and of fungal strains – on Saburo medium with the use of standard commercial discs.
Results and discussion The array and level of resistance to antibacterial drugs in clinical isolates of microorganisms of different taxonomic groups obtained from patients in outpatient care was established. The research has established that 43,3 % Staphylococcus spp. isolates possessed polyantibiotic resistance and 3,8 % – extensive resistance, only sensitivity to aminoglycosides and glycopeptides was preserved. The majority of beta-haemolytic streptococci was moderately resistant to macrolides (85,7 %), levofloxacine (52,4 %) and chloramphenicole (66,7 %) and resistant to clindamycine (90,5 %). All isolates of E. faecalis demonstrated moderate resistance to II and III generation nitrofurane and chinolone derivatives and a quarter of isolates – to ampycilline. More than a half of clinical isolates of P. aeruginosa (58,3 %) demonstrated polyantibiotic resistance. A quarter of all obtained isolates of P. aeruginosa possessed extensive resistance, preserving sensitivity to only monobactames and carbapenemes. Among Enterobacteriaceae spp. 44,4 % of strains were polyresistant. One strain of E. сoli isolated from genital tract had extensive resistance. The majority of studied С. albicans fungal strains were sensitive to all studied antifungal agents. The percent of strains resistant to antifungal agents was between 8,6 and 17,1 %.  Conclusion In 30,5 % of all studies clinical isolates multiple drug resistance was observed. Polyantibiotic resistance was show in 58,3 % P. aeruginosa isolates, 44,4 % Enterobacteriaceae spp. strains and in 43,3 % studied Staphylococcus spp. isolates. According to the study results, the significant portion of antibiotic resistant and polyresistant strains of Staphylococcus spp., P. aeruginosa and microorganisms of Enterobacteriaceae family circulating in outpatient conditions are probable producers of beta-lactamases.

Посилання

The world health report 2014 – World health statistics 2014 [Electronic resource] / World Health Organization. – Mode of access : http://apps.who.int/iris/bitstream/10665/112738/1/9789240692671_eng.pdf.

The scourge of antibiotic resistance: the important role of the environment [Text] / R. L. Finley, P. Collignon, D. G. Larsson [et al.] // Clin. Infect. Dis. – 2013. – Vol. 57. – № 5. – P. 704–710.

Tolstanov, O. K. The priority tasks of pediatrics education and science in the health care reform [Теxt] / O. K. Tolstanov // Сhild’s Health. – 2013. – № 5. – С. 11–17.

Feshchenko, Yu. I. Antibiotic resistance of microorganisms. State of problem and way of decision [Text] / Yu.I. Feshchenko, M.I. Gumenuk, O.S. Denisov // Ukrainian chemotherapeutic journal. – 2010. – № 1–2 (23). – P. 4–10.

Svizhak, V. K. Antibiotic resistance: many-sided nature of problem [Text] / V. K. Svizhak, S. E. Deyneka // Clinical & Experimental Pathology. – 2014. – Vol. XIII. – № 2 (48). – С. 222–224.

Vatanskya, І. Yu. Antibiotic resistance (Review) [Text] / І. Yu. Vatanskya // Ukrainian journal «Surgery of Donbass». – 2012. – Vol. 1. – № 2. – P. 73–81.

Kozlov, R. S. Selection of resistance associated with the use of antimicrobial agents: collateral damage concept [Text] / R. S. Kozlov // Clin. Microbiol., Antimicrob. Chemother. – 2010. – Vol. 12. – № 4. – P. 284–292.

Angebault, C. Antimicrobial agent exposure and the emergence and spread of resistant microorganisms: issues associated with study design [Text] / C. Angebault, А. Andremont // Eur. J. Clin. Microbiol. Infect. Dis. – 2013. – Vol. 32. – № 5. – P. 581–595.

Effect of antibiotic prescribing in primary care on antimicrobial resistance in individual patients: systematic review and meta-analysis [Text] / C. Costelloe, С. Metcalfe, А. Lovering [et al.] // BMJ. – 2010. – Vol. 340. – P. 20–96.

Carbapenem-resistant Enterobacteriaceae: a review of tratment and outcomes [Text] / D. Van Duin, K. S. Kaye, E. A. Neuner, R. A. Bonomo // Diagn. Microbiol. Infect. Dis. – 2013. – Vol. 75. – № 2. – Р. 115–120.

Impact of previous antibiotic therapy on outcome of Gramnegative severe sepsis [Text] / M. T. Johnson, R. Reichley, J. Hoppe-Bauer [et al.] // Crit. Care Med. – 2011. – Vol. 39. – № 8. – P. 1859–1865.

Rivera, A. M. Current concepts in antimicrobial therapy against select gram-positive organisms: methicillin-resistant Staphylococcus aureus, penicillin-resistant Pneumococci, and vancomycin-resistant Enterococci [Text] / A. M. Rivera, H. W. Boucher // Mayo Clin. Proc. – 2011. – Vol. 86. – № 12. – P. 1230–1243.

Extensively drug-resistant Pseudomonas aeruginosa: risk of bloodstream infection in hospitalized patients [Text] / C. Peña, S. Gómez-Zorrilla, С. Suarez [et al.] // Eur. J. Clin. Microbiol. Infect. Dis. – 2012. – Vol. 31. – № 10. – P. 2791–2797.

Hlumcher, F. S. Multiresistant infections: relevance, definition, mechanisms, prevailing pathologens, treatment, prevention [Тext] / F. S. Hlumcher, S. O. Dubrov, Y. L. Kuchyn // Interdepartmental Medical Journal «Science & Practice». – 2014. – № 1 (2). – P. 129–149.

Prevalence, resistance mechanisms, and susceptibility of multidrug-resistant bloodstream isolates of Pseudomonas aeruginosa / V. H. Tam, K. T. Chang, К. Abdelraouf [et al.] // Antimicrob. Agents Chemother. – 2010. – Vol. 54. – № 3. –` P. 1160–1164.

Multidrug-resistant pathogens in hospitalised patients coming from the community with pneumonia: a European perspective [Text] / S. Aliberti, C. Cilloniz, J. D. Chalmers [et al.] // Thorax. – 2013. – Vol. 68. – № 11. – P. 997–999.

Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance [Text] / A. P. Magiorakos, А. Srinivasan, R. B. Carey [et al.] // Clin. Microbiol. Infect. – 2012. – Vol. 18. – P . 268–281.

About claim of the methodical pointing in relation to determination of sensitiveness of microorganisms to antibacterial preparations [Text] : order № 167. – [operating from 05.04.2007] – Kyiv : Ministry of Health Care of the Ukraine, 2007. – 63 p.

About standartization of microbiological (bacteriological) methods of research, medical prophylactic establishments applied in clinical diagnostic laboratories [Text] : order № 535. – [operating from 22.04.1985] – Moscow : Ministry of Health Care of the USSR, 1985. – 65 p.

Standartization of the preparation of microbial suspensions / Y. L. Volyanskiy, L. G. Mironenko, S. V. Kalinichenko [et al.] // Newwsletter of innovations in health care № 163–2006. Ministry of Health Care of the Ukraine ; Ukranian Centre for Scientific Health Information and Patent licensing work (Ukrmedpatentinform), Kyiv, 2006.

Sutton, D. Determinant of pathogenic and opportunistic fungi [Теxt] / D. Sutton, D. Fothergilla.– М. : Мir, 2001. – 486 p.

##submission.downloads##

Як цитувати

Osolodchenko, T., Andreieva, I., Zavada, N., Lukyanenko, T., Batrak, O., & Ryabova, I. (2020). Sensitivity to antibacterial drugs in agents of community-acquired infections. Анали Мечниковського Інституту, (1), 29–38. вилучено із https://journals.uran.ua/ami/article/view/192139

Номер

Розділ

Дослідні статті