The study of the biological properties of probiotic lactobacillus spp. strains under aerobic and microaerophilic cultivation conditions

Ye Babych; S Kalinichenko; O Korotkykh; T Ryzhkova; N Sklyar; I Maslii; A Balak; N Shkodovska; M Bagacha

Автор(и)

Ye Babych Mechnikov Institute of Microbiology and Immunology,
S Kalinichenko Mechnikov Institute of Microbiology and Immunology,
O Korotkykh Mechnikov Institute of Microbiology and Immunology,
T Ryzhkova Mechnikov Institute of Microbiology and Immunology,
N Sklyar Mechnikov Institute of Microbiology and Immunology,
I Maslii Institute of Experimental and Clinical veterinary medicine,
A Balak Kharkov National Medical university,
N Shkodovska Mechnikov Institute of Microbiology and Immunology,
M Bagacha Mechnikov Institute of Microbiology and Immunology,

Ключові слова:

probiotic strains, lactobacteria, adhesive properties, growth characteristics, susceptibility to antibiotics

Анотація

The biological properties (growth characteristics, adhesive activity and sensitivity to antimicrobial) of probiotic Lactobacillus strains were studied under different gas composition of incubation atmosphere. It was found that the number of viable lactobacilli cells in the one dose of investigated probiotic preparations was lower than it was claimed by the manufacturer. Gas composition of incubation atmosphere affects cell viability of probiotic strains. The number of colony forming units of lactobacilli under microaerophilic conditions increased in 1,19-1,33 times as compared with aerobic conditions. It was proved that adhesive activity of probiotic Lactobacillus strains and sensitivity to 2th, 3th, 4th generations of cephalosporins (cefuroxime, cefotaxime, cefepime) and tetracyclines (doxycycline) also increased under microaerophilic conditions. The changes of the biological properties of lactobacilli under different cultivation conditions require further study for optimization of correction of dysbiotic disorders.

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