Immunobiological properties of medicinal product obtained on the basis of strains of Bifidobacteria and Lactobacilli

O Khizhnyak

Автор(и)

O Khizhnyak National Technical University "Kharkov Polytechnic Institute",

Ключові слова:

prebiotic component, microflora, probiotics, sterile filtrate of culture fluid, immunopotentiating activity, antibacterial activity, safety.

Анотація

The article treats issues of examination of safety of combined bacterial medicinal product on the basis of probiotic strains of bacteria belonging to two genera obtained by submerged mutual cultivation. During conducting experiments, it was found that the medicinal product causes no changes in blood hematological parameters of experimental animals, does not lead to their death, has no immunotoxic action, histological examination shows no deviations from the norm. In comparison with standard medicinal products, the proposed consortium of the said strains of bifidobacteria and lactobacilli does not differ by its safety from commercially available medicinal products “Bifidumbacterin” and “Lactobacterin” produced by PrJSC “Biofarma” but provides higher parameters of biological activity.
There also was conducted examination of culture fluid activity after removal of proposed bacterial consortium. A high antibacterial activity of the sterile filtrate of culture fluid was found.

Посилання

The modern state of development and application of probiotics, prebiotics and synbiotics [Text] / S.V. Kalinichenko [et al.] // Annals of Mechnikov Institute. – 2013. - № 3. – P. 5-12.

The normal microbiota of the human gastrointestinal tract: history of analysis, succession, and dietary influences [Text] / A.L. McCartney, G.R. Gibson // Gastrointestinal Microbiology. – 2006. - №2. – Р. 51–73.

Probiotics and gastrointestinal disease: Clinical evidence and basic science [Text] / E.O. Petrof // Antiinflammatory, antiallergy agents medical chemistry. – 2009. - №3. – P.260 – 269.

Probitics in gastrointestinal and liver diseases [Text] / D. Jonkers // Aliment. Pharmacol. Ther. – 2007. - №26. – P. 133 – 148.

Khizhnyak, O.S. Prebiotics – required component of combined probiotic medicinal product [Text] / O.S. Khizhnyak// Problems and Prospects of Science Development: Materials of VIII International Research-to-Practice Conference. – Chernivtsi, 2013. – P. 180 – 181.

Lactitol prebiotic – an effective product for maintenance of health of modern man [Text] / S.I. Artyukhova, Yu.A. Gavrilova // International Magazine of Applied and Fundamental Research. – 2013. – № 4. - P. 95-96.

Artyukhova, S.I. Use of probiotics and prebiotics in biotechnology designed for production of bioproducts [Text] / S.I. Artyukhova, Yu. A. Gavrilova. – O. : PHofOSTU, 2010. – 112 p.

"Eksportal" (Lactitol) - safe and effective osmotic laxative [Text] / O.P. Minushkin [et.al] // RJGGK. - 2010. - № 5. - P.118.

Effects of lactitol on intestinal microflora and plasma endotoxin in patients with chronic viral hepatitis [Text] / C.Chen [et al] // Infection. - 2007. - № 54(1). – P. 98-102.

Metabolic effects and their role in functioning of gastrointestinal tract of patients suffering from celiacia [Text] / L.S. Oreshko [et al] // Gastroenterology. – 2012. – № 1. - P. 27-28.

New opportunities for prevention and treatment of chronic constipation complex prebiotic agents: choice of optimal combination [Text] / L.I.Butorova // Gastroenterology. - 2012. - № 4. - P. 2-10.

Prebiotic properties of lactitol and lactulose for creating symbiotic bacteria [Text] / M. A. Harhota [et al] // Mіkrobіol. Journal. – 2012. - №1. – P. 52 – 58.

Prebiotic lactitol as a component of a biological product of aerobic bacilli [Text] / L.A.Safronova [et al] // Mіkrobіol. Journal. - 2008. - № 6. - P. 34-41.

Lactitol or lactulose in the treatment of chronic constipation: result of a systematic review [Text] / A. Maydeo // Indian Med. Assoc. – 2010. - №11. – P. 789 – 792.

Bondarenko, V. M. Molecular and cellular mechanisms of therapeutic action of probiotics [Text] / V. M. Bondarenko // Farmateka. - 2010. -№ 2. – P. 26-32.

Khizhnyak, O.S. Joint cultivation of probiotic strains of lactobacilli and bifidobacteria [Text] / O.S Khiznyak // Scientific Discussion: Issues of Mathematics, Physics, Chemistry, Biology: Proceedings of II International Conference. – Moscow, 2013. – P. 87 – 91.

Commensal gut bacteria: mechanism of immune modulation [Text] / D.Kelly [et al] // Trends Immunology. - 2005. - №26. – P 326 - 333.

Experimental evaluation limfotsitotoksic action of bifidobacteria and lactobacillus [Text] / A.S. Erdyakova [et al] // Practical Medicine. - 2012. - №3. – P.194-196.

Examination of some immunological parameters of blood serum of experimental animals during their pregnancy period [Text] / L.I. Drozdova, N.V. Arkhipenko // Agrarian Messenger of the Ural. - 2011.- №9. - P. 8-12.

Immunomodulatory activity of lacto- and bifidobacteria probiotic strains in vitro and in vivo [Text] / L. M. Lazarenko [et al.] // Theses of ХІІІth congress of Vynograds'kyj Society of Microbiologists of Ukraine. – Yalta, 2013. – P. 278.

Chicherin, I.Yu. Investigation of the influence of large doses of probiotic microorganisms and Bifidumbacterin autoflora intestinal organism of white mice and indicators of cellular immunity [Electronic resource] / I. Yu. Chicherin [et al] // Intestinal microflora: Collection of scientific articles. – 2013. - №2. – P. 61 – 66. Access mode: http://gastroportal.ru/files/mikroflora.pdf

Comparative evaluation of microbial survival of probiotics in selling drugs in in vitro [Text] / I.V. Darmov // Experimental and clinical gastroenterology. - 2011. - № 9. - P. 96-101.